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Mutants of human ACE2 differentially promote SARS-CoV and SARS-CoV-2 spike mediated infection.
Shukla N, Roelle SM, Suzart VG, Bruchez AM, Matreyek KA
PLoS Pathog. 2021 Jul 16;17(7):e1009715. doi: 10.1371/journal.ppat.1009715. eCollection 2021 Jul.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
171588pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter
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171594AttB_ACE2_IRES-mCherry-H2A-P2A-PuroRStrong human ACE2 abundance from Bxb1 landing pad
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171595AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroRWeak human ACE2 abundance from Bxb1 landing pad
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171596AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroREctodomain deleted negative control ACE2 from Bxb1 landing pad
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171597AttB _EGFPStrong EGFP fluorescence from Bxb1 landing pad
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171598AttB_mCherryStrong mCherry fluorescence from Bxb1 landing pad
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171599pLenti_CMV-EGFP-2A-mNeonGreenGreen lentiviral vector plasmid for pseudovirus experiments
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Antibodies from Article