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AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR
(Plasmid #171594)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 171594 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Custom
  • Vector type
    Mammalian Expression, Synthetic Biology ; Promoterless Bxb1 DNA recombination
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ACE2
  • Species
    H. sapiens (human)
  • Entrez Gene
    ACE2 (a.k.a. ACEH)

Gene/Insert 2

  • Gene/Insert name
    mCherry

Gene/Insert 3

  • Gene/Insert name
    H2A

Gene/Insert 4

  • Gene/Insert name
    Pac

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR was a gift from Kenneth Matreyek (Addgene plasmid # 171594 ; http://n2t.net/addgene:171594 ; RRID:Addgene_171594)
  • For your References section:

    Mutants of human ACE2 differentially promote SARS-CoV and SARS-CoV-2 spike mediated infection. Shukla N, Roelle SM, Suzart VG, Bruchez AM, Matreyek KA. PLoS Pathog. 2021 Jul 16;17(7):e1009715. doi: 10.1371/journal.ppat.1009715. eCollection 2021 Jul. 10.1371/journal.ppat.1009715 PubMed 34270613