Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice.
Ling S, Yang S, Hu X, Yin D, Dai Y, Qian X, Wang D, Pan X, Hong J, Sun X, Yang H, Paludan SR, Cai Y
Nat Biomed Eng. 2021 Jan 4. pii: 10.1038/s41551-020-00656-y. doi: 10.1038/s41551-020-00656-y. PubMed Article
Ling S, Yang S, Hu X, Yin D, Dai Y, Qian X, Wang D, Pan X, Hong J, Sun X, Yang H, Paludan SR, Cai Y
Nat Biomed Eng. 2021 Jan 4. pii: 10.1038/s41551-020-00656-y. doi: 10.1038/s41551-020-00656-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166031 | pMS2M-PH-gagpol-D64V | Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol. |
| 166032 | pMDlg/pRRE-int-MS2M | Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol. |
| 166033 | pCMV-2NLS-Cas9-6XMS2 | Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles. |
| 166034 | pLV-egfp-U3-Osp.gRNA | Empty backbone for expression of gRNA-expressing integration deficient lentiviral particles. |