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Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans.
Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, Frokjaer-Jensen C
Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
159798pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]Expression construct with ubiquitous expression to test fluorescence level on microscope
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159799pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]Expression construct with ubiquitous expression to test fluorescence level on microscope
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159800pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]Expression construct with ubiquitous expression to test fluorescence level on microscope
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159801pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]Expression construct with ubiquitous expression to test fluorescence level on microscope
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159802pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]Expression construct with ubiquitous expression to test fluorescence level on microscope
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159803pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]Expression construct with ubiquitous expression to test fluorescence level on microscope
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159804pCFJ1358 - Intron GG1 - smu-1Golden Gate compatible intron with PATCs for reduced germline silencing
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159805pCFJ1359 - Intron GG2 - smu-1Golden Gate compatible intron with PATCs for reduced germline silencing
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159806pCFJ1360 - Intron GG3 - smu-1Golden Gate compatible intron with PATCs for reduced germline silencing
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159807pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]Optimized enzyme for genome editing in C. elegans
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159808pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]Optimized enzyme for genome editing in C. elegans
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159809pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]Optimized enzyme for genome editing in C. elegans
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159810pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]Optimized enzyme for genome editing in C. elegans
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159811pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]Optimized enzyme for genome editing in C. elegans
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159813pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]Optimized enzyme for genome editing in C. elegans
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159814pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]Optimized enzyme for genome editing in C. elegans
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159815pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]Optimized enzyme for genome editing in C. elegans
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159816pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]Optimized enzyme for genome editing in C. elegans
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159817pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]Positive control for germline GFP expression from simple array in C. elegans
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159838pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays
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159843pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159844pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159845pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159846pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159847pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159848pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159849pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159850pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159851pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159852pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159853pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159854pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159855pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159856pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159857pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159858pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159859pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159860pCFJ2241 - [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159861pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159862pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159863pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159864pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159865pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159866pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159867pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159869pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159870pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)Three-fragment Gateway compatible flourophore for expression in C. elegans
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159872pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)Three-fragment Gateway compatible protein tag for expression in C. elegans
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159873pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)Three-fragment Gateway compatible protein tag for expression in C. elegans
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159874pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)Three-fragment Gateway compatible protein tag for expression in C. elegans
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159877pCFJ2369 - intron GG1 - 250bpGolden Gate compatible intron with PATCs for reduced germline silencing
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159878pCFJ2359 - intron GG2 - 250bpGolden Gate compatible intron with PATCs for reduced germline silencing
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159879pCFJ2370 - intron GG3 - 250bpGolden Gate compatible intron with PATCs for reduced germline silencing
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159880pCFJ2214 - Intron GG1 - 900 bpGolden Gate compatible intron with PATCs for reduced germline silencing
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159881pCFJ2259 - Intron GG2 - 900 bpGolden Gate compatible intron with PATCs for reduced germline silencing
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159882pCFJ2215 - Intron GG3 - 900 bpGolden Gate compatible intron with PATCs for reduced germline silencing
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159883pCFJ2365 - Intron GG1 - 250bp no PATCGolden Gate compatible intron with no PATCs for negative controls
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159884pCFJ2345 - Intron GG2 - 250bp no PATCGolden Gate compatible intron with no PATCs for negative controls
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159885pCFJ2366 - intron GG3 - 250bp no PATCGolden Gate compatible intron with no PATCs for negative controls
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159886pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays
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159887pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays
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161516pCFJ1149 - intron 3 smu-2 PATCsPATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans
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Antibodies from Article