PurposeVector expressed CasRx and sgRNA_LACZ for AAV package
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||154003||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)3848
- Promoter EFS
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
INSERT: CasRx driven by EFS promoter.
INSERT: DR-SgRNA_LacZ driven by U6 promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR was a gift from Hui Yang (Addgene plasmid # 154003 ; http://n2t.net/addgene:154003 ; RRID:Addgene_154003)
For your References section:Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. He B, Peng W, Huang J, Zhang H, Zhou Y, Yang X, Liu J, Li Z, Xu C, Xue M, Yang H, Huang P. Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2. 10.1007/s13238-020-00700-2 PubMed 32185621