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AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR
(Plasmid #154001)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 154001 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CasRx
  • Species
    Synthetic
  • Insert Size (bp)
    3849
  • Promoter GFAP, U6
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

INSERT: CasRx driven by GFAP promoter.
INSERT: DR-SgRNA_Ptbp1 driven by U6 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR was a gift from Hui Yang (Addgene plasmid # 154001 ; http://n2t.net/addgene:154001 ; RRID:Addgene_154001)
  • For your References section:

    Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. Zhou H, Su J, Hu X, Zhou C, Li H, Chen Z, Xiao Q, Wang B, Wu W, Sun Y, Zhou Y, Tang C, Liu F, Wang L, Feng C, Liu M, Li S, Zhang Y, Xu H, Yao H, Shi L, Yang H. Cell. 2020 Apr 30;181(3):590-603.e16. doi: 10.1016/j.cell.2020.03.024. Epub 2020 Apr 8. 10.1016/j.cell.2020.03.024 PubMed 32272060