Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag.
Wilhelm J, Kuhn S, Tarnawski M, Gotthard G, Tunnermann J, Tanzer T, Karpenko J, Mertes N, Xue L, Uhrig U, Reinstein J, Hiblot J, Johnsson K
Biochemistry. 2021 Aug 24;60(33):2560-2575. doi: 10.1021/acs.biochem.1c00258. Epub 2021 Aug 2. PubMed Article
Wilhelm J, Kuhn S, Tarnawski M, Gotthard G, Tunnermann J, Tanzer T, Karpenko J, Mertes N, Xue L, Uhrig U, Reinstein J, Hiblot J, Johnsson K
Biochemistry. 2021 Aug 24;60(33):2560-2575. doi: 10.1021/acs.biochem.1c00258. Epub 2021 Aug 2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167266 | pET51b-His-TEV-HaloTag7 | Overexpression of HaloTag7 in E. coli |
| 167267 | pET51b-His-TEV-HaloTag7_dead_mt | Overexpression of the dead variant (D106A) of HaloTag7 in E. coli |
| 167268 | pET51b-His-TEV-HOB_(HaloTag7_oligo_binder) | Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli |
| 167269 | pET51b-His-TEV-SNAP-tag | Overexpression of SNAP-tag in E. coli |
| 167270 | pET51b-His-TEV-SNAP-tag_dead_mt | Overexpression of the dead variant (C145A) of SNAP-tag in E. coli |
| 167271 | pET51b-His-TEV-SNAP-tag_fast | Overexpression of the fast variant (E30R) of SNAP-tag in E. coli |
| 167272 | pET51b-His-TEV-SNAP-tag_fast_dead_mt | Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli |
| 167273 | pET51b-His-TEV-SNAP-tag_cx | Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli |
| 167274 | pET51b-His-TEV-CLIP-tag | Overexpression of CLIP-tag in E. coli |
| 167275 | pET51b-His-TEV-CLIP-tag_fast | Overexpression of the fast variant (E30R) of CLIP-tag in E. coli |
| 167276 | pET51b-Strep-hAGT-His | Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli |
| 167277 | pET51b-Strep-SsOGT-H5-His | Overexpression of a sulfolobal equivalent of SNAP-tag in E. coli |