pET51b-Strep-hAGT-His
(Plasmid #167276)

Purpose

Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli
Depositing Lab

Kai Johnsson
Publication

Wilhelm et al bioRxiv April 13, 2021 ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	167276	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pET51b(+)
(Search Vector Database)
Backbone manufacturer
Novagen
Vector type
Bacterial Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
Low Copy

Gene/Insert

Gene/Insert name
hAGT
Species
H. sapiens (human)
Insert Size (bp)
744
Entrez Gene
AGT (a.k.a. ANHU, SERPINA8, hFLT1)
Promoter T7
Tags / Fusion Proteins
- Strep-tag (N terminal on insert)
- His x10 (C terminal on insert)

Cloning Information

Cloning method Gibson Cloning

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pET51b-Strep-hAGT-His was a gift from Kai Johnsson (Addgene plasmid # 167276 ; http://n2t.net/addgene:167276 ; RRID:Addgene_167276)
For your References section:

Kinetic and structural characterization of the self-labeling protein tags HaloTag7, SNAP-tag and CLIP-tag. Wilhelm J, Kuehn S, Tarnawski M, Gotthard G, Tuennermann J, Taenzer T, Karpenko J, Mertes N, Xue L, Uhrig U, Reinstein J, Hiblot J, Johnsson K. bioRxiv April 13, 2021 10.1101/2021.04.13.439540

pET51b-Strep-hAGT-His (Plasmid #167276)