PARP1 FL
(Plasmid #169815)

• Purpose: Expresses codon optimised full length His tagged PARP1 in bacterial cells
• Depositing Lab: David Neuhaus
• Publication: Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):2266-2288. doi: 10.1093/nar/gkab020.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 8314
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Poly[ADP-ribose] polymerase 1
• Alt name: PARP1
• Alt name: ADPRT
• Alt name: PPOL
• Species: H. sapiens (human), Synthetic; Codon optimised
• Insert Size (bp): 3042
• Mutation: Valine 762 mutated to an alanine
• GenBank ID: NP_001609
• Entrez Gene: PARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • MKHHHHHHMKQ (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original codon optimised PARP-1 gene was purchased from Qiagen, this is identical to the submitted plasmid with the exception of the V762A mutation

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry