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173,134 results

Showing: 8421 - 8440 of 173134 results

  5. pLenti-CMV-Pink Flamindo-p2a-bPAC

    Plasmid
    #202716
    Purpose
    Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Inserts
    Pink Flamindo
    bPAC
    Use
    Lentiviral
    Tags
    10xHis and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    202716-plasmid-map-sequence-id-402843

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  8. pLyGo-Ec-1

    Plasmid
    #163127
    Purpose
    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Ubiquitin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163127-plasmid-map-sequence-id-331491

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  9. R777-E283 Hs.ROCK1

    Plasmid
    #70567
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70567-plasmid-map-sequence-id-126016

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  12. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  13. pCDNA3.3_CoV2_B.1.1.7

    Plasmid
    #170451
    Purpose
    Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Yuan et al Science. 2021 Aug 13;373(6556):818-823.
    Insert
    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
    Tags
    NA
    Expression
    Mammalian
    Mutation
    HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …
    Promoter
    CMV
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170451-plasmid-map-sequence-id-333038

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  14. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161928
    Purpose
    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    Surface marker and Citrine activation reporter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pEF
    Available Since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161928-plasmid-map-sequence-id-325287

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  17. G3BP1-Grx1-roGFP2

    Plasmid
    #236548
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in stress granules.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    G3BP1-Grx1-roGFP2 (G3BP1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236548-plasmid-map-sequence-id-470046

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Showing: 8421 - 8440 of 173134 results