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pcDNA-p160MBP
(Plasmid #41)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 myc-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p160 myb binding protein
  • Alt name
    Mybbp1a
  • Alt name
    p160MBP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4000
  • GenBank ID
    BC052889
  • Entrez Gene
    Mybbp1a (a.k.a. AL024407, AU019902, P160, p160MBP, p67MBP)
  • Tag / Fusion Protein
    • myc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tom Gonda
  • Terms and Licenses

Depositor Comments

There is a K935R mutation in this insert. The mutation is not known to affect p160MBP function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-p160MBP was a gift from Bruce Spiegelman (Addgene plasmid # 41)
  • For your References section:

    Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK. Fan M, Rhee J, St-Pierre J, Handschin C, Puigserver P, Lin J, Jaeger S, Erdjument-Bromage H, Tempst P, Spiegelman BM. Genes Dev 2004 Feb 1;18(3):278-89. Epub 2004 Jan 26. 10.1101/gad.1152204 PubMed 14744933