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psfGFP-LAMP1-mCherry (pHLARE)
(Plasmid #164477)


Item Catalog # Description Quantity Price (USD)
Plasmid 164477 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Entrez Gene
    Lamp1 (a.k.a. LGP120)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • superfolder GFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psfGFP-LAMP1-mCherry (pHLARE) was a gift from Diane Barber (Addgene plasmid # 164477 ; ; RRID:Addgene_164477)
  • For your References section:

    pHLARE: A New Biosensor Reveals Decreased Lysosome pH in Cancer Cells. Webb BA, Aloisio FM, Charafeddine RA, Cook J, Wittmann T, Barber DL. Mol Biol Cell. 2020 Nov 25:mbcE20060383. doi: 10.1091/mbc.E20-06-0383. 10.1091/mbc.E20-06-0383 PubMed 33237838