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Yannick Doyon Lab Plasmids


The Yannick Doyon Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009.
A marker-free co-selection strategy for high efficiency human genome engineering Daniel Agudelo, Lusine Bozoyan, Alexis Duringer, Caroline C. Huard, Sophie Carter, Jeremy Loehr, Dafni Synodinou, Mathieu Drouin, Jayme Salsman, Graham Dellaire, Josee Laganiere, Yannick Doyon bioRxiv. 15 Mar 2017.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
68375AAVS1_Puro_PGK1_3xFLAG_Twin_StrepMammalian Expression, CRISPR, TALEN ; ZFNA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging. pUC19
  • Tags / Fusion Proteins
    • 3xFLAG_Twin_Strep (N terminal on backbone)
    • 3xFLAG_Twin_Strep (C terminal on backbone)
  • Add to Cart
    79877eSpCas9(1.1)_No_FLAGMammalian Expression, CRISPRA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid. pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Tag / Fusion Protein
    • Untagged eSpCas9(1.1)
  • Add to Cart
    79888eSpCas9(1.1)_No_FLAG_AAVS1_T2Mammalian Expression, CRISPRA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus. pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Tag / Fusion Protein
    • Untagged eSpCas9(1.1)
  • Add to Cart
    79891FancF_N_FS_v2_DonorCRISPRA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep pUC19
  • Tag / Fusion Protein
    • 3xFLAG_Twin_Strep
  • Add to Cart
    79892SP_gRNA_pUC19_N_FancF_LeftFancF_Site_1_Left (Homo sapiens)CRISPRA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Human gRNA expression vector targeting FANCF (site 1 left) pUC19 Add to Cart
    79899EZH2-TALEN_Left_No_FLAGEZH2-TALEN_Left_No_FLAGMammalian Expression, TALENA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2 JDS78 Add to Cart
    79900EZH2-TALEN_Right_No_FLAGEZH2-TALEN_Right_No_FLAG (Homo sapiens)TALENA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2 EZH2-TALEN_Right_No_FLAG Add to Cart
    79901EZH2_N_FS_v2_DonorCRISPR, TALENA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep pUC19
  • Tag / Fusion Protein
    • 3xFLAG_Twin_Strep (N terminal on insert)
  • Add to Cart
    79902AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH23xFLAG_Twin_Strep_EZH2 (Homo sapiens)Mammalian Expression, CRISPR, TALENA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Plasmid #68375) Add to Cart
    86551ATP1A1_plasmid_donor_RDATP1A1 (Homo sapiens)Mammalian Expression, CRISPR, TALEN ; Co-selection via HDR using ouabainA marker-free co-selection strategy for high efficiency human genome engineering bioRxiv. 15 Mar 2017. Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain pUC19 Add to Cart
    86610eSpCas9(1.1)_No_FLAG_ATP1A1_G2ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1) (Other)Mammalian Expression, CRISPR ; Co-selection via NHEJ using ouabainA marker-free co-selection strategy for high efficiency human genome engineering bioRxiv. 15 Mar 2017. Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 Add to Cart
    86612eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNAATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1) (Other)Mammalian Expression, CRISPR ; Co-selection via NHEJ using ouabainA marker-free co-selection strategy for high efficiency human genome engineering bioRxiv. 15 Mar 2017. Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 Add to Cart
    86613eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNAATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1) (Other)Mammalian Expression, CRISPR ; Co-selection via HDR using ouabainA marker-free co-selection strategy for high efficiency human genome engineering bioRxiv. 15 Mar 2017. Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 Add to Cart
    86617pY036_ATP1A1_G3ATP1A1 G3 crRNA + AsCpf1-3xHA (Other)Mammalian Expression, CRISPR ; Co-selection via NHEJ using ouabainA marker-free co-selection strategy for high efficiency human genome engineering bioRxiv. 15 Mar 2017. Expresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1 pY036-U6-crRNA(BbsI)-CBh-AsCpf1 Add to Cart
    86618pY036_ATP1A1_G5ATP1A1 G5 crRNA + AsCpf1-3xHA (Other)Mammalian Expression, CRISPR ; Co-selection via HDR using ouabainA marker-free co-selection strategy for high efficiency human genome engineering bioRxiv. 15 Mar 2017. Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1 pY036-U6-crRNA(BbsI)-CBh-AsCpf1 Add to Cart
    86619pY036_ATP1A1_G3_ArrayATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA (Other)Mammalian Expression, CRISPR ; Co-selection via NHEJ using ouabainA marker-free co-selection strategy for high efficiency human genome engineering bioRxiv. 15 Mar 2017. Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid pY036-U6-crRNA(BbsI)-CBh-AsCpf1 Add to Cart
    86620pY036_ATP1A1_G5_ArrayATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA (Other)Mammalian Expression, CRISPR ; Co-selection via HDR using ouabainA marker-free co-selection strategy for high efficiency human genome engineering bioRxiv. 15 Mar 2017. Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid pY036-U6-crRNA(BbsI)-CBh-AsCpf1 Add to Cart