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Yannick Doyon Lab Plasmids


The Yannick Doyon Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, Doyon Y Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
68375AAVS1_Puro_PGK1_3xFLAG_Twin_StrepMammalian Expression, CRISPR, TALEN ; ZFNA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging. pUC19
  • Tags / Fusion Proteins
    • 3xFLAG_Twin_Strep (N terminal on backbone)
    • 3xFLAG_Twin_Strep (C terminal on backbone)
  • Add to Cart
    79877eSpCas9(1.1)_No_FLAGMammalian Expression, CRISPRA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid. pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Tag / Fusion Protein
    • Untagged eSpCas9(1.1)
  • Add to Cart
    79888eSpCas9(1.1)_No_FLAG_AAVS1_T2Mammalian Expression, CRISPRA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus. pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Tag / Fusion Protein
    • Untagged eSpCas9(1.1)
  • Add to Cart
    79891FancF_N_FS_v2_DonorCRISPRA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep pUC19
  • Tag / Fusion Protein
    • 3xFLAG_Twin_Strep
  • Add to Cart
    79892SP_gRNA_pUC19_N_FancF_LeftFancF_Site_1_Left (Homo sapiens)CRISPRA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Human gRNA expression vector targeting FANCF (site 1 left) pUC19 Add to Cart
    79899EZH2-TALEN_Left_No_FLAGEZH2-TALEN_Left_No_FLAGMammalian Expression, TALENA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2 JDS78 Add to Cart
    79900EZH2-TALEN_Right_No_FLAGEZH2-TALEN_Right_No_FLAG (Homo sapiens)TALENA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2 EZH2-TALEN_Right_No_FLAG Add to Cart
    79901EZH2_N_FS_v2_DonorCRISPR, TALENA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep pUC19
  • Tag / Fusion Protein
    • 3xFLAG_Twin_Strep (N terminal on insert)
  • Add to Cart
    79902AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH23xFLAG_Twin_Strep_EZH2 (Homo sapiens)Mammalian Expression, CRISPR, TALENA Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Plasmid #68375) Add to Cart