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Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Levskaya A, Weiner OD, Lim WA, Voigt CA
Nature. 2009 Oct 15;461(7266):997-1001.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
22275pAL149Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
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22276pAL175Expresses YFP-PIF for red-light-induced binding to Phy
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22277pAL188Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment
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22278pAL189Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment
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22279pAL190Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment
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22280pAL197
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22281pAL198
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22282pAL204Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
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22471pAL113Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.
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22474pAL101Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy
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22475pAL130Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy
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Antibodies from Article