We narrowed to 10,185 results for: yeast

1. pcDNA3-Lifeact-Dronpa

   Plasmid

   #182330

   Purpose

   Actin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   Lifeact-Dronpa
   

   Tags

   N-terminal 17 amino acids from budding yeast Abp1…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pADH140

   Plasmid

   #90988

   Purpose

   NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Inserts

   C. maltosa LEU2 1 of 2
   pENO1
   Cas9
   NAT 1 of 2
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDN0604 (pDEST, HygR)

   Plasmid

   #210483

   Purpose

   pDEST vector with HygR marker for gene expression in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDN0603 (pDEST, NatR)

   Plasmid

   #210482

   Purpose

   pDEST vector with NatR marker for gene expression in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCu-MCP-2yeGFP(405)

   Plasmid

   #121419

   Purpose

   Expressing MCP-2yeGFP

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

   Insert

   CUP1p-MCP-2yeGFP
   

   Expression

   Yeast

   Promoter

   CUP1 promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-SKII-URA-GAL1-fCLIP-3xHA

   Plasmid

   #188936

   Purpose

   Integration cassette to create N-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   URA-GAL1-fCLIP-3xHA
   

   Expression

   Yeast

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWS926

   Plasmid

   #123052

   Purpose

   LexA bacterial repressor

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   LexA-NLS [3a]
   

   Expression

   Yeast

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6-mTFP1-KanMX6

   Plasmid

   #69851

   Purpose

   C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mTFP1
   

   Expression

   Yeast

   Available Since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEINO5

   Plasmid

   #179489

   Purpose

   Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

   Depositor

   Bogumil Karas

   Article

   Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

   Insert

   aacC1
   

   Use

   Synthetic Biology; Bacterial and yeast cloning

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMJ235: pRS426-His-MBP-GtFz1_Native

    Plasmid

    #205254

    Purpose

    S. cerevisiae expression vector for GtFz1 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    GtFz1
    

    Tags

    10xHis, MBP

    Expression

    Yeast

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBKWH

    Plasmid

    #133898

    Purpose

    Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLexA::Ras

    Plasmid

    #11340

    Depositor

    Michael Wigler

    Article

    Chang et al Cell. 1994 Oct 7. 79(1):131-41.

    Insert

    Ras1 (ras1 Fission Yeast)
    

    Tags

    LexA DNA binding domain

    Expression

    Yeast

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pDDGFP-Leu2d-ScVrg4

    Plasmid

    #102334

    Purpose

    Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

    Insert

    YGL225W (VRG4 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSDMA65

    Plasmid

    #128354

    Purpose

    Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    ACT1

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYC34

    Plasmid

    #63915

    Purpose

    Recipient overexpressing vector to construct mCherry tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    TEF1 (Saccharomyces cerevisiae)

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-URA3-3'SEC13-GFP

    Plasmid

    #25445

    Depositor

    Benjamin Glick

    Article

    Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

    Insert

    Sec13-Green Fluorescent Protein
    

    Expression

    Yeast

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKar2-msGFP-HDEL

    Plasmid

    #117668

    Purpose

    Localisation of Endoplasmic Reticulum

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pKar2-msGFP-HDEL
    

    Expression

    Yeast

    Mutation

    Contains the Kar2 promoter and signal sequence fr…

    Promoter

    pKar2

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPTK002-2-pGAP

    Plasmid

    #84961

    Purpose

    Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Glyceraldehyde-3-phosphate dehydrogenase promoter
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    BsaI site removed (1805 c to g)

    Promoter

    Non

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRG710_lexO-HO_LexA-TF_HIS3MX

    Plasmid

    #154819

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. POM42-mCherry

    Plasmid

    #69856

    Purpose

    N-terminal tagging with mCherry using URA3 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCherry minus start and stop codon
    

    Expression

    Yeast

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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