Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

CUP Ent2 YFP p316
(Plasmid #15593)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15593 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p316 CUP
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ENT2
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ENT2 (a.k.a. YLR206W)
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Figure 5 in article.

Lindquist lab number: 8028

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CUP Ent2 YFP p316 was a gift from Susan Lindquist (Addgene plasmid # 15593 ; http://n2t.net/addgene:15593 ; RRID:Addgene_15593)
  • For your References section:

    A network of protein interactions determines polyglutamine toxicity. Duennwald ML, Jagadish S, Giorgini F, Muchowski PJ, Lindquist S. Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11051-6. 10.1073/pnas.0604548103 PubMed 16832049