We narrowed to 14,953 results for: pET

1. RhoC gRNA #1

   Plasmid

   #198724

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #1

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RhoC gRNA #2

   Plasmid

   #198725

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #2

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RhoC gRNA #3

   Plasmid

   #198726

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #3

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEMS2075

   Plasmid

   #158098

   Purpose

   High Copy number plasmid containing smCBA insert.

   Depositor

   Elizabeth Simpson

   Article

   de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

   Insert

   smCBA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   smCBA

   Available since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pQE30-DARPin-E11

   Plasmid

   #226784

   Purpose

   Expression of His-tagged DARPin E11 in E. coli

   Depositor

   Sebastian Mathea

   Article

   Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

   Insert

   DARPin E11
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJB50

   Plasmid

   #226311

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with V(T/S)G to AAA CsoS2 MR1-7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJB55

   Plasmid

   #226310

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with V(T/S)G to AAA CsoS2 MR1-5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221076

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221077

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221079

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

    Plasmid

    #221082

    Purpose

    Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    CMV, T7

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA

    Plasmid

    #220225

    Purpose

    AAV-STARR-seq validation vector containing VS33 enhancer

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Insert

    Enhancer (VS33)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

    Plasmid

    #212809

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Tags

    NUbo and mCherry-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

    Plasmid

    #212804

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-P2A-3xFLAG-Ubc7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG

    Plasmid

    #212803

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Tags

    NUbo and mCherry-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Halotag-6xHp

    Plasmid

    #214414

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to Halotag

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (SPAST Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mApple-1xHp

    Plasmid

    #214409

    Purpose

    Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    Spastin aa 43-92 (SPAST Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-Creb5(HA)

    Plasmid

    #195022

    Purpose

    Gateway vector containing HA-tagged Creb5

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
    

    Use

    Gateway cloning

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GL4.10 E2E1- Prg4 promoter-luciferase

    Plasmid

    #195035

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4.10 E3E2E1-Prg4 promoter-luciferase

    Plasmid

    #195036

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge