Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support.
Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033. PubMed Article
Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85083 | pPdR | Expresses putidaredoxin reductase in E. coli |
| 85084 | pPdX | Expresses putidaredoxin in E. coli |
| 85085 | pP450cam | Expresses P450cam (CYP101A1) in E. coli |
| 85086 | pR1G108CP20X | Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli |
| 85087 | pC3 | Expresses PCNA3 protein fused to P450cam (CYP101A1) in E. coli |
| 85088 | pC3R112C/T180C | Expresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coli |
| 85089 | p1G108CFa | Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli |
| 85090 | p3R112C/T180CBM3 | Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli |
| 85091 | p3R112C/T180CHFm | Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli |
| 85092 | p1G108C-GG | Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C) |
| 85093 | p3R112C/T180C-GG | Host vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C) |
| 85094 | pGS-3 | Host vector to fuse a gene at the N-terminus of PCNA3 |
| 85095 | pGS-3R112C/T180C | Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C) |
| 85096 | p1G108CP20X | Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli |
| 85097 | pHPro51 | 1st Pro5 |
| 85098 | pHPro52 | 2nd Pro5 |
| 85099 | pHPro53 | 3rd Pro5 |
| 85100 | pBsaPro5X | Putidaredoxin fused to Pro5 |
| 85101 | pBsaP450camGG | P450cam (CYP101A1) |
| 85102 | pBsaBM3 | P450 BM3 variant (A74G/C773S/C810S) |
| 85103 | pBsaHFm | Heme-FMN domain of P450 BM3 variant (A74G) |
| 85104 | pBsaFa | FAD domain of P450 BM3 variant (C773S/C810S) |