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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,121 results for: gibson assembly

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Showing: 881 - 900 of 1121 results

  1. C84m

    Plasmid
    #120995
    Purpose
    MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 28
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120995-plasmid-map-sequence-id-236105

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  2. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

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  3. C63m

    Plasmid
    #120979
    Purpose
    MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lacI
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120979-plasmid-map-sequence-id-236033

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  4. P60m

    Plasmid
    #120952
    Purpose
    MoClo golden gate assembly AB for pSalTTTC (Adam Meyer's improved pSal promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pSal Adam Meyer
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120952-plasmid-map-sequence-id-235966

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  5. C3m

    Plasmid
    #120956
    Purpose
    MoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)
    Depositor
    Richard Murray
    Insert
    sfGFP
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120956-plasmid-map-sequence-id-235970

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  6. P30m

    Plasmid
    #120942
    Purpose
    MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pL-lacO1
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120942-plasmid-map-sequence-id-238038

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  7. pEND-350_Cacnes_ori

    Plasmid
    #225605
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Publication
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225605-plasmid-map-sequence-id-451372

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  8. U9m

    Plasmid
    #121022
    Purpose
    MoClo golden gate assembly BC part for BCD22 (very low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD22
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121022-plasmid-map-sequence-id-236185

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  9. U17m

    Plasmid
    #121023
    Purpose
    MoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bujard RBS
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121023-plasmid-map-sequence-id-245898

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  10. T2m

    Plasmid
    #121029
    Purpose
    MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    ECK120033736 (164.6x Voigt terminator), short attachment
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121029-plasmid-map-sequence-id-238076

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  11. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

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  12. T14m

    Plasmid
    #121033
    Purpose
    MoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S1P13 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121033-plasmid-map-sequence-id-236356

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  13. C113m

    Plasmid
    #121012
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Cas9
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121012-plasmid-map-sequence-id-236320

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  14. U2m

    Plasmid
    #121015
    Purpose
    MoClo golden gate assembly BC part for BCD11 (Medium-high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD11
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121015-plasmid-map-sequence-id-236174

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  15. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

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  16. C38m

    Plasmid
    #120971
    Purpose
    MoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase active at transition from exponential phase to stationary phase). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 38 (RpoS)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120971-plasmid-map-sequence-id-236023

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  17. P32m

    Plasmid
    #120943
    Purpose
    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    PlacO (Tim Lu, Matt Bennett, etc)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120943-plasmid-map-sequence-id-236887

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  18. P33m

    Plasmid
    #120944
    Purpose
    MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P11 from Mutalik library (medium)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120944-plasmid-map-sequence-id-245421

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  19. P34m

    Plasmid
    #120945
    Purpose
    MoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P13 from Mutalik library (weak)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120945-plasmid-map-sequence-id-238039

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  20. P58m

    Plasmid
    #120950
    Purpose
    MoClo golden gate assembly AB for pLas-A-10b (Adam Meyer's improved pLas promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pLas Adam Meyer
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120950-plasmid-map-sequence-id-235962

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Showing: 881 - 900 of 1121 results