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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,107 results for: gibson assembly

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Showing: 881 - 900 of 1107 results

  1. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

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  2. T14m

    Plasmid
    #121033
    Purpose
    MoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S1P13 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121033-plasmid-map-sequence-id-236356

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  3. C113m

    Plasmid
    #121012
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Cas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121012-plasmid-map-sequence-id-236320

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  4. U2m

    Plasmid
    #121015
    Purpose
    MoClo golden gate assembly BC part for BCD11 (Medium-high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD11
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121015-plasmid-map-sequence-id-236174

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  5. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

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  6. C38m

    Plasmid
    #120971
    Purpose
    MoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase active at transition from exponential phase to stationary phase). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 38 (RpoS)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120971-plasmid-map-sequence-id-236023

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  7. P32m

    Plasmid
    #120943
    Purpose
    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    PlacO (Tim Lu, Matt Bennett, etc)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120943-plasmid-map-sequence-id-236887

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  8. P33m

    Plasmid
    #120944
    Purpose
    MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P11 from Mutalik library (medium)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120944-plasmid-map-sequence-id-245421

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  9. P34m

    Plasmid
    #120945
    Purpose
    MoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P13 from Mutalik library (weak)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120945-plasmid-map-sequence-id-238039

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  10. P58m

    Plasmid
    #120950
    Purpose
    MoClo golden gate assembly AB for pLas-A-10b (Adam Meyer's improved pLas promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pLas Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120950-plasmid-map-sequence-id-235962

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  11. P59m

    Plasmid
    #120951
    Purpose
    MoClo golden gate assembly AB part for pLux76B (Adam Meyer's improved pLux promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pLux Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120951-plasmid-map-sequence-id-236155

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  12. pFP.R375

    Plasmid
    #138245
    Purpose
    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138245-plasmid-map-sequence-id-273498

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  17. pUC19-B2KB46

    Plasmid
    #103086
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B2KB46(Cas9 coding gene from Elusimicrobium minutum (strain Pei191))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    May 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103086-plasmid-map-sequence-id-209052

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  18. pUC19-J5TNY4

    Plasmid
    #103116
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    J5TNY4(Cas9 coding gene from Porphyromonas sp. oral taxon 279 str. F0450)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103116-plasmid-map-sequence-id-201878

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  19. pUC19-K0XCK7

    Plasmid
    #103118
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    K0XCK7(Cas9 coding gene from Barnesiella intestinihominis YIT 11860)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103118-plasmid-map-sequence-id-201880

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  20. pUC19-Q1QGC9

    Plasmid
    #103121
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    • Q1QGC9(Cas9 coding gene from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103121-plasmid-map-sequence-id-201883

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Showing: 881 - 900 of 1107 results