We narrowed to 1,069 results for: gibson assembly

1. pUC19-E6KTN8

   Plasmid

   #103076

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   E6KTN8(Cas9 coding gene from Actinomyces sp. oral taxon 180 str. F0310)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-A1IQ68

   Plasmid

   #103080

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   A1IQ68(Cas9 coding gene from Neisseria meningitidis serogroup A / serotype 4A (strain Z2491))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-A5EIM8

   Plasmid

   #103081

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   A5EIM8(Cas9 coding gene from Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-A7HP89

   Plasmid

   #103082

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   A7HP89(Cas9 coding gene from Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-B5JU35

   Plasmid

   #103087

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   B5JU35(Cas9 coding gene from gamma proteobacterium HTCC5015)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC19-B5XLC1

   Plasmid

   #103088

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   B5XLC1(Cas9 coding gene from Streptococcus pyogenes serotype M49 (strain NZ131))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19-C4ZA16

   Plasmid

   #103090

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   C4ZA16(Cas9 coding gene from Eubacterium rectale (strain ATCC 33656 / VPI 0990))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5/FRT/TO - RBM28 E8 G>T minigene

   Plasmid

   #169272

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 8 G>T minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 E8 G>T minigene (RBM28 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NM_018077.2:c.(946G>T)

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 WT minigene

   Plasmid

   #169273

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 1-6 WT minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 Exon1-6 WT minigene (RBM28 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

    Plasmid

    #169274

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 Exon1-6 GT>A minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NM_018077.2:c.(541+1_541+2delinsA)

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. plentiCRISPR-sgMDH1

    Plasmid

    #72875

    Purpose

    The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgMDH1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pOSV801

    Plasmid

    #126044

    Purpose

    modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

    Depositor

    Jean-Luc Pernodet

    Article

    Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid

    #132927

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Insert

    humanized SpCas9
    

    Use

    CRISPR and Synthetic Biology; Pmagic gateway entr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHRSV_CHARMS

    Plasmid

    #233920

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONOR 6colors

    Plasmid

    #229974

    Purpose

    bigMamAct assembled plasmid for 6colors cellular labelling

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid

    #206259

    Purpose

    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    GTS mTagBFP
    

    Use

    Multimate/gateway entr 4

    Tags

    BFGT1 (Golgi localisation peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMMACE DEST CMV H2B iRFP

    Plasmid

    #206253

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B iRFP713
    

    Use

    Recombinant baculovirus production, multimate/gat…

    Tags

    iRFP713

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMMACE DEST CMV SpCas9

    Plasmid

    #206268

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    SpCas9
    

    Use

    CRISPR; Recombinant baculovirus production

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a-SRRD-V5-APEX2 (in pLEX_307)

    Plasmid

    #214921

    Purpose

    constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMMACE DEST CMV PE2

    Plasmid

    #206276

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    PE2
    

    Use

    CRISPR; Recombinant baculovirus production, multi…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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