Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,083 results for: sgRNA

Showing: 8981 - 9000 of 9083 results

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pBHM2329

Plasmid

#171686

Purpose

pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

C. neoformans U6

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_ABE_D3_empty

Plasmid

#203809

Purpose

Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

ABE_D3

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_PT1_empty

Plasmid

#203810

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6_PT1

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CreER-pA_FRT-acry-venus

Plasmid

#241166

Purpose

Middle entry vector for mini-Golden subcloning platform; creER; acry-venus

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER; acry-venus

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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P2A-CreER-pA-ORF1-FRT-acry mCherry

Plasmid

#241167

Purpose

Middle entry vector for mini-Golden subcloning platform; creER

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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P2A-CreER-pA-ORF2-FRT-acry mCherry

Plasmid

#241168

Purpose

Middle entry vector for mini-Golden subcloning platform; creER

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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P2A-CreER-pA-ORF3-FRT-acry mCherry

Plasmid

#241169

Purpose

Middle entry vector for mini-Golden subcloning platform; creER

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF3

Plasmid

#241182

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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nAPEX2-V5-zdCas9n-3x-FLAG-GSG-linker-P2A-mNeongreen

Plasmid

#241191

Purpose

Middle entry vector for mini-Golden subcloning platform; 3x-FLAG; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

3x-FLAG; mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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3x-FLAG-nzdCas9-V5-APEX2n-GSG-linker-P2A-mNeongreen

Plasmid

#241190

Purpose

Middle entry vector for mini-Golden subcloning platform; V5-APEX2n; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

V5-APEX2n; mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF3

Plasmid

#241194

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF2

Plasmid

#241193

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF1

Plasmid

#241192

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF1

Plasmid

#241180

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF2

Plasmid

#241181

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-1

Plasmid

#200539

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-2

Plasmid

#200540

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3

Plasmid

#200541

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_CreER-pA-FRT-acry:venus-pA-FRT

Plasmid

#200543

Purpose

mini circle middle entry plasmid for CreER-pA-FRT-acry:venus-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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