We narrowed to 15,992 results for: LEA

1. NR4A1

   Plasmid

   #25477

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NR4A1 (NR4A1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pET22b-EC12WTC9AAvi

   Plasmid

   #47925

   Purpose

   Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

   Depositor

   Tanja Kortemme

   Article

   Ritterson et al J Am Chem Soc. 2013 Aug 7.

   Insert

   E-Cadherin EC12
   

   Tags

   6xHis, Avi Tag, and TEV Cleavage

   Expression

   Bacterial

   Mutation

   changed Cysteine 9 to Alanine

   Promoter

   T7

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC-HIS6-THRX-TEV-GG

   Plasmid

   #215796

   Purpose

   Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. mWasabi-LaminB1-10

   Plasmid

   #56507

   Purpose

   Localization: Nuclear Envelope, Excitation: 493, Emission: 509

   Depositor

   Michael Davidson

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   mWasabi

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. RPRD1B

   Plasmid

   #40739

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RPRD1B (RPRD1B Human)
   

   Tags

   6xHis TEV

   Expression

   Bacterial

   Available Since

   Nov. 14, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pET24a MDHC1_ARATH

   Plasmid

   #204237

   Purpose

   Bacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHC1_ARATH (AT1G04410 Mustard Weed)
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PANK1

   Plasmid

   #32871

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PANK1 (PANK1 Human)
   

   Tags

   His tag (with Thrombin cleavage)

   Expression

   Bacterial

   Mutation

   amino acids 231-597 only

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pUPD2 PhyB (GB2721)

   Plasmid

   #160603

   Purpose

   chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.

   Depositor

   Diego Orzaez

   Insert

   PhyB
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGAT2

   Plasmid

   #112588

   Purpose

   Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-GST fusions

   Depositor

   Johan Peränen, Marko Hyvönen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His-GST

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. LeGO-Cer

    Plasmid

    #27351

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    U6 promoter, SFFV promoter, Cerulean
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAT3

    Plasmid

    #112589

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-GST fusions

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-GST

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAT4

    Plasmid

    #112585

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SMYD3

    Plasmid

    #32048

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SMYD3 (SMYD3 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. SPIN2B (SPIN2BA-c011)

    Plasmid

    #98238

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPIN2BA (SPIN2B Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. UBC64

    Plasmid

    #25143

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ubc64.248.414 (UBE2Q1 Human)
    

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    contains UBC domain; residues 248-414

    Available Since

    Oct. 31, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pET28a MDH_PFALCI

    Plasmid

    #204235

    Purpose

    Bacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTagRFP-T2-LaminB1-10

    Plasmid

    #58040

    Purpose

    Localization: Nuclear Envelope, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mTagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. 21 pCOLI_A_N-GST_C_STREP

    Plasmid

    #160258

    Purpose

    N-term GST 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII and GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSETA_ionRE

    Plasmid

    #172926

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRE
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid

    #50417

    Purpose

    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.

    Depositor

    Andrew Scharenberg

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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