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pGBKT7-PRP11

Plasmid

#87594

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp11

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP11 (PRP11 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-MUD2

Plasmid

#87588

Purpose

Encodes Y2H Gal4 DNA binding domain for Mud2

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

MUD2 (MUD2 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-HSH49

Plasmid

#87573

Purpose

Encodes Y2H Gal4 DNA binding domain for Hsh49

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH49 (HSH49 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-CUS2

Plasmid

#87558

Purpose

Encodes Y2H Gal4 DNA binding domain for Cus2

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

CUS2 (CUS2 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-CUS1

Plasmid

#87557

Purpose

Encodes Y2H Gal4 DNA binding domain for Cus1

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

CUS1 (CUS1 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 G409E

Plasmid

#87279

Purpose

Encodes Hsh155 G409E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

G409E

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS415 Prp2 Q548N

Plasmid

#87604

Purpose

Encodes Prp2 Q548N for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP2 (PRP2 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Q548N

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 D450G

Plasmid

#87281

Purpose

Encodes Hsh155 D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

D450G

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 P369K

Plasmid

#87278

Purpose

Encodes Hsh155 P369K for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

P369K

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 K335T

Plasmid

#87276

Purpose

Encodes Hsh155 K335T for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

K335T

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414 Hsh155 K335R

Plasmid

#87275

Purpose

Encodes Hsh155 K335R for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

K335R

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414 Hsh155 K335N

Plasmid

#87274

Purpose

Encodes Hsh155 K335N for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

K335N

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414 Hsh155 H331Q

Plasmid

#87272

Purpose

Encodes Hsh155 H331Q for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

H331Q

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 H331D

Plasmid

#87271

Purpose

Encodes Hsh155 H331D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

H331D

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414 Hsh155 N295D

Plasmid

#87270

Purpose

Encodes Hsh155 N295D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N295D

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414 Hsh155 E291D

Plasmid

#87266

Purpose

Encodes Hsh155 E291D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

E291D

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJK040 (pET-PurQ-A128T)

Plasmid

#73840

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Mutation

A128T mutant

Promoter

T7

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK044 (pET-PurQ-wt)

Plasmid

#73839

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 RRN3

Plasmid

#67112

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RRN3 (RRN3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFAP4

Plasmid

#67073

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFAP4 (TFAP4 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

P338A

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only