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pFlag-CMV4-PHF13

Plasmid

#246094

Purpose

Expression of human PHF13 with Flag-tag in mammalian cells

Depositor

Sarah Kinkley

Publication

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (PHF13 Human)

Tags

Flag

Expression

Mammalian

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-d7F

Plasmid

#245784

Purpose

Expression of mutated bovine arrestin-3 (deleted residues 175, 177, 178, 182-185) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Publication

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

deleted residues 175, 177, 178, 182-185

Promoter

CMV

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583)

Plasmid

#239260

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3584)

Plasmid

#239261

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RPPH1 (pAVA3585)

Plasmid

#239262

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPPH1

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPPH1

Use

CRISPR and Lentiviral

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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POC1553

Plasmid

#238470

Purpose

pMOBC360_VL: Vector Left (VL) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-21_MBP-VidN

Plasmid

#241013

Purpose

VidN construct that can be bacterially expressed, used to demonstrate intein splicing reaction.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

MBP-VidN

Tags

MBP-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb847

Plasmid

#217502

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb867

Plasmid

#217503

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb897

Plasmid

#217505

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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POC1554

Plasmid

#238471

Purpose

pMOBC360_VR: Vector Right (VR) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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H1x

Plasmid

#218111

Purpose

Encodes mus musculus histone 1 in Gateway entry vector

Depositor

Peter Hamilton

Publication

Kim et al Transl Psychiatry. 2024 Jun 5;14(1):239.

Insert

Histone H1x (H1f10 Mouse)

Use

Gateway cloning entry vector

Expression

Bacterial

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–2×GFP NB(WT)

Plasmid

#232304

Purpose

KR GFP adaptor for C. elegans

Depositor

Masato Kanemaki

Publication

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

KR GFP adaptor
OsTIR1(F74G)

Expression

Worm

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(WT)–GFP NB(WT)

Plasmid

#232297

Purpose

WT GFP nanbody adaptor

Depositor

Masato Kanemaki

Publication

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

WT GFP adaptor
OsTIR1(F74G)

Expression

Mammalian

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–GFP NB(KR)

Plasmid

#232298

Purpose

KR GFP nanobody adaptor

Depositor

Masato Kanemaki

Publication

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Type

Empty backbone

Expression

Mammalian

Available since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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POC1525

Plasmid

#226496

Purpose

pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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POC1520

Plasmid

#221581

Purpose

pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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POC1355

Plasmid

#221572

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1343, POC1344, POC1345 and POC1346; respectively

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1430

Plasmid

#221577

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1426, POC1427, POC1428 and POC1429; respectively

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1518

Plasmid

#221579

Purpose

pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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