167,948 results

1. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. evoFERNY-Cas9NG

   Plasmid

   #203329

   Purpose

   Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   evoFERNY-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCsA

   Plasmid

   #136067

   Purpose

   Acceptor plasmid for even level position 1, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pHLUM

   Plasmid

   #40276

   Depositor

   Markus Ralser

   Article

   Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

   Inserts

   LEU2 (LEU2 Budding Yeast)
   URA3 (URA3 Budding Yeast)
   MET15/17 (MET17 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   endogenous LEU2, endogenous MET15/17, and endogen…

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFPC1-EPSIN 2

   Plasmid

   #22242

   Depositor

   Pietro De Camilli

   Article

   Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

   Insert

   Epsin 2 (EPN2 Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

6. CD44P pGL3

   Plasmid

   #19122

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   CD44 promoter (CD44 Human)
   

   Use

   Non viral cloning vector

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-NtLNtC60αC20C60β

   Plasmid

   #160874

   Purpose

   Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, cpn20
   

   Expression

   Bacterial

   Promoter

   rbcL: pBAD, chaperones: T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRT/TO/HIS/FLAG/HA-PGK1

   Plasmid

   #38071

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   PGK1 phosphoglycerate kinase 1 (PGK1 Human)
   

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-CaNAR2

   Plasmid

   #64728

   Purpose

   FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNAR2
   

   Tags

   Cerulean3 and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRN114

    Plasmid

    #127221

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, Wus, AtSTM
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LcV2-Hygro

    Plasmid

    #91977

    Purpose

    LentiCRISPR v2 with Hygro cloned in place of Puro

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Integrin-Alpha2-N-18

    Plasmid

    #58192

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: N/A, Emission: N/A

    Depositor

    Michael Davidson

    Insert

    Integrin-Alpha2 (ITGA2 Human)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. NRCoC

    Plasmid

    #74300

    Purpose

    Mammalian expression of full-length treacle fused to GFP

    Depositor

    Rita Shiang

    Article

    Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

    Insert

    treacle ribosome biogenesis factor 1 (Tcof1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. XLone-SOX18

    Plasmid

    #163822

    Purpose

    expresses human SOX18 upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    SOX18 (SOX18 Human)
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLB1080

    Plasmid

    #37826

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    ATP7B (ATP7B Human)
    

    Tags

    mEGFP

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMV_AA088

    Plasmid

    #216104

    Purpose

    Knockout, EF1a-driven Cas9 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-MITF-A

    Plasmid

    #38132

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-A (MITF Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-mBaoJin-P2A-FusionRed

    Plasmid

    #229572

    Purpose

    Expresses mBaoJin and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-AtC60αβC20NtL

    Plasmid

    #160876

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Expression

    Bacterial

    Promoter

    rbcL: T7, chaperones: T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJJS002

    Plasmid

    #188325

    Purpose

    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::GFP::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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