215,948 results

1. pcDNA3.4_CEACAM6-His

   Plasmid

   #149337

   Purpose

   for eukaryotic expression and purification of CEACAM6-His6

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Tags

   IL-2 signal peptide and polyhistidine

   Expression

   Mammalian

   Mutation

   point mutation (GGA to GCA) to replace 'G-g…

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJeM1

   Plasmid

   #135088

   Purpose

   rhamnose-inducible expression vector for replication in E. coli and P. putida

   Depositor

   Josef Altenbuchner

   Article

   Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

   Insert

   rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-MHL

   Plasmid

   #26096

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET28-MHL

   Plasmid

   #26096

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA_RFP-TEVprotease

   Plasmid

   #124947

   Purpose

   Expression of RFP-TEVprotease

   Depositor

   Daniel Hammer, Benjamin Schuster, Matthew Good

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RFP-TEVprotease
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1α-mNeonGreen-Z1

   Plasmid

   #135613

   Purpose

   mNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.

   Depositor

   Jordan Green

   Insert

   mNeonGreen
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a-HTLV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP

   Plasmid

   #179636

   Purpose

   TEVP-inducible single TM SEAP RELEASE construct

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   SEAP RELEASE
   

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOlyA(E69A)

   Plasmid

   #132392

   Purpose

   Mutant version of OlyA that senses sphingomyelin without or with cholesterol

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A point mutant (E69A)
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, E69A, C94S, S151C

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3XFlagNICD2

   Plasmid

   #20184

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 2 intracellular domain (Notch2 Mouse)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains murine Notch 2 Intracellular Domain (Met…

   Promoter

   CMV

   Available Since

   Jan. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3XFlagNICD2

    Plasmid

    #20184

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 2 intracellular domain (Notch2 Mouse)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains murine Notch 2 Intracellular Domain (Met…

    Promoter

    CMV

    Available Since

    Jan. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Talin1-mCherry

    Plasmid

    #212004

    Purpose

    Expression of mouse full-length (1-2541) talin1-mCherry in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

    Insert

    Mouse talin1 (Tln1 Mouse)
    

    Tags

    mCherry fluorescent protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF3358

    Plasmid

    #144834

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)

    Viral Prep

    #104492-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pTwist GFP-Linker-VDAC1

    Plasmid

    #211735

    Purpose

    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-SMARCA4

    Plasmid

    #65391

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-SMARCA4

    Plasmid

    #65391

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-2xFLAG-Atoh1

    Plasmid

    #101734

    Purpose

    Expresses mouse Atoh1 with FLAG tag

    Depositor

    Albert Edge

    Article

    Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Tags

    2x FLAG

    Expression

    Mammalian

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. ND4.2-Left TALE-G1397-N-DddA11-mCherry

    Plasmid

    #179682

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    S1330I A1341V N1342S E1370K T1380I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK344 (Hygro-P2A-mAID)

    Plasmid

    #121178

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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