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Inactivation of Ezh2 Upregulates Gfi1 and Drives Aggressive Myc-Driven Group 3 Medulloblastoma.
Vo BT, Li C, Morgan MA, Theurillat I, Finkelstein D, Wright S, Hyle J, Smith SM, Fan Y, Wang YD, Wu G, Orr BA, Northcott PA, Shilatifard A, Sherr CJ, Roussel MF
Cell Rep. 2017 Mar 21;18(12):2907-2917. doi: 10.1016/j.celrep.2017.02.073. PubMed Article

Plasmids from Article

ID Plasmid Purpose
91877AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFPExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1
91879AOI-WT-Cas9-sg-mouse Ezh2-E10-GFPExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2
91880AOI-WT-Cas9-sg-mouse Ezh2-E18-GFPExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2
91881AOI-WT-Cas9-sg-mouse Suz12-F1-GFPExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12
91882AOI-WT-Cas9-sg-mouse Suz12-F2-GFPExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12
91883AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFPExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1
91884AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFPExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1
91885MSCV-mouse Ezh2 delta SETdomain-IRES-GFPRetroviral expression of mouse Ezh2 with a SET domain deletion
91887MSCV-mouse Ezh2 Y637S-IRES-GFPRetroviral expression of mouse Ezh2 with Y637S
91888MSCV-human Erbb2-IRES-GFPRetroviral expression of human Erbb2
91889MSCV-human HoxB4-IRES-YFPRetroviral expression of human HoxB4
91890MSCV-human Igf2bp2-IRES-GFPRetroviral expression of human Igfbp2
91891MSCV-mouse Gfi1-IRES-GFPRetroviral expression of mouse Gfi1
91892pCDH-CMV-mouse Gfi1-GFP3rd generation lentiviral expression of mouse Gfi1
91893pT2K-CAGGS-mouse Gfi1-IRES-GFPMammalian expression of mouse Gfi1

Antibodies from Article