PurposeLentiviral expression of YFP-tagged LC3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||99571||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
SpeciesH. sapiens (human)
Entrez GeneMAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
/ Fusion Protein
- YFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byOpen reading frames were obtained from other labs.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLVX-LC3-YFP was a gift from Edward Campbell (Addgene plasmid # 99571 ; http://n2t.net/addgene:99571 ; RRID:Addgene_99571)
For your References section:Endocytic vesicle rupture is a conserved mechanism of cellular invasion by amyloid proteins. Flavin WP, Bousset L, Green ZC, Chu Y, Skarpathiotis S, Chaney MJ, Kordower JH, Melki R, Campbell EM. Acta Neuropathol. 2017 May 19. doi: 10.1007/s00401-017-1722-x. 10.1007/s00401-017-1722-x [pii] PubMed 28527044