pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1
(Plasmid
#183951)
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PurposeInducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLIX_403 (Addgene 41395)
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Backbone manufacturerRoot Lab, Addgene
- Backbone size w/o insert (bp) 9396
- Total vector size (bp) 9152
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Modifications to backboneLR insertion of place-holder gene, followed by restriction cloning and Gibson assembly to isolate APOBEC1 and insert Capture1.
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAPOBEC1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)687
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Entrez GeneApobec1 (a.k.a. REPR, apobec-1)
- Promoter TRE promoter, Tet ON
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer LNCX
- 3′ sequencing primer O.PGK1b-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1 was a gift from Eugene Yeo (Addgene plasmid # 183951 ; http://n2t.net/addgene:183951 ; RRID:Addgene_183951) -
For your References section:
Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes. Brannan KW, Chaim IA, Marina RJ, Yee BA, Kofman ER, Lorenz DA, Jagannatha P, Dong KD, Madrigal AA, Underwood JG, Yeo GW. Nat Methods. 2021 May;18(5):507-519. doi: 10.1038/s41592-021-01128-0. Epub 2021 May 7. 10.1038/s41592-021-01128-0 PubMed 33963355
Map uploaded by the depositor.
