We narrowed to 10,190 results for: yeast

1. pTSK561

   Plasmid

   #190816

   Purpose

   For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

   Depositor

   Tatiana Karpova, Gunjan Mehta

   Article

   Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

   Insert

   HaloTag
   

   Use

   Bacterial cloning vector

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDIV537

   Plasmid

   #177704

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Promoter

   RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNA0306

   Plasmid

   #169452

   Purpose

   pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid

   Depositor

   Jef Boeke

   Article

   Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

   Insert

   Cas9
   

   Expression

   Yeast

   Promoter

   TEF1p

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKMV-VioD

   Plasmid

   #65350

   Purpose

   systhesis of vioD gene in the violacein pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   vioD
   

   Use

   Synthetic Biology

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRoC30_Th3.14

   Plasmid

   #188068

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAWH-largeT

   Plasmid

   #133895

   Purpose

   Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   LargeT
   

   Expression

   Yeast

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKMV-VioB

   Plasmid

   #65348

   Purpose

   systhesis of vioB gene in the violacein pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   vioB
   

   Use

   Synthetic Biology

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRoC30_Th3

   Plasmid

   #188064

   Purpose

   PROSS-designed high-redox potential laccase from Trametes hirsuta

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   PROSS-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 mutations, described in publication

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRoC30_Tv9nL

   Plasmid

   #188069

   Purpose

   PROSS-designed high-redox potential laccase from Trametes versicolor

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   PROSS-designed high-redox potential laccase from Trametes versicolor
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   79 PROSS mutations, described in publication

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB3045(gRNA XI-3)

    Plasmid

    #73287

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS416 Gal M337V YFP

    Plasmid

    #27449

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pGAN201

    Plasmid

    #129204

    Purpose

    yTRAP sensor of [RNQ+], detects aggregation of Rnq1

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    RNQ1 (RNQ1 Synthetic, Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    SUP35

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPICZ-pre-pro-alphaf-E2-Crimson

    Plasmid

    #117658

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf-E2-Crimson
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. POT2-RFP

    Plasmid

    #65318

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGZ173 (pFA6a-SL-MNase-kanMX6)

    Plasmid

    #70234

    Purpose

    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

    Depositor

    Steven Henikoff

    Article

    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

    Insert

    Short linker and aa 83-231 of Mnase
    

    Use

    Yeast genomic targeting

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS416 Gal G294A YFP

    Plasmid

    #27448

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pDN0606 (pDEST-DHFR F[3] N-term,HygR)

    Plasmid

    #210488

    Purpose

    pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

    Plasmid

    #210485

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

    Plasmid

    #210487

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKMV-VioC

    Plasmid

    #65349

    Purpose

    systhesis of vioC gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioC
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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