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pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3b

Plasmid

#59421

Purpose

Expresses lncRNA MEG3 isoform b in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3b (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3a

Plasmid

#59420

Purpose

Expresses lncRNA MEG3 isoform a in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3a (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3c

Plasmid

#59422

Purpose

Expresses lncRNA MEG3 isoform c in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3c (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3d

Plasmid

#59423

Purpose

Expresses lncRNA MEG3 isoform d in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3d (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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CX-nestinGFPas

Plasmid

#32401

Depositor

Patrick Salmon, Henryk Zulewski

Article

Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

Insert

nestin promoter/enhancer GFP

Use

Lentiviral

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-LacI

Plasmid

#183920

Purpose

Lac repressor labeled with GFP; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

GFP-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

SV2

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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CAP-AAV.FUS.3

Plasmid

#222333

Purpose

AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

Depositor

Mikhail Shapiro, Jerzy Szablowski

Article

Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

Insert

AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1

Use

AAV

Promoter

p41

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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MBD2a pcDNA3.1

Plasmid

#78141

Purpose

Mammalian expression of MBD2a

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD2a (MBD2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP207-AAV-CMV-MCS-pA

Plasmid

#78318

Purpose

AAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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ClCry4 PHR

Plasmid

#160552

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 HR

Tags

Hexa his

Expression

Insect

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

Plasmid

#126553

Purpose

Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

Depositor

Jonathan Karn

Article

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

Thy1.2 and GFP

Promoter

HIV LTR

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP3A4

Plasmid

#67104

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP3A4 (CYP3A4 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP2D6

Plasmid

#67090

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP2D6 (CYP2D6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAPM-D4- miR30-L1221

Plasmid

#115846

Purpose

Control shRNA KD vector

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

control shRNA

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MBD2b pcDNA3.1

Plasmid

#78142

Purpose

Mammalian expression of MBD2b

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD2b (MBD2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
tdTomato-LacI

Plasmid

#183921

Purpose

Lac repressor labeled with tdTomato; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdTomato-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

Plasmid

#191338

Purpose

Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX-TCRb-FKBP-Blasticidin

Plasmid

#120723

Purpose

Expresses ER-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

T cell receptor β chain

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-p18-CIBN

Plasmid

#188990

Purpose

A lentiviral plasmid encoding lysosome-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Tags

p18

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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