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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 643 results for: pFAST

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Showing: 81 - 100 of 643 results

  1. pFastBacMam5_N_Flag_His8_eGFP_3C

    Plasmid
    #178816
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Tags
    Flag, His8, eGFP, 3C
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178816-plasmid-map-sequence-id-351927

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  2. pFastBacMam3_N_His8_eGFP_3C

    Plasmid
    #178807
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Tags
    His8, eGFP, 3C
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178807-plasmid-map-sequence-id-351924

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  3. pFastBacMam6_C_3C_His8_Stop

    Plasmid
    #178810
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Tags
    3C, His8, Stop codon
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178810-plasmid-map-sequence-id-351923

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  4. pFastBacMam7_N_Flag_TEV

    Plasmid
    #178817
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Tags
    Flag, TEV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178817-plasmid-map-sequence-id-351922

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  7. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    p10 and polyhedrin
    Available Since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65214-plasmid-map-sequence-id-116341

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  10. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  14. pFastBacMam8_N_Flag_3C

    Plasmid
    #178818
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Tags
    Flag, 3C
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178818-plasmid-map-sequence-id-351926

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  17. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Article
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + T…
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186626-plasmid-map-sequence-id-366906

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  18. pFastBac1-Nucleocapsid

    Plasmid
    #179696
    Purpose
    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells
    Depositor
    Mark Arbing
    Insert
    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    His6-TEV protease site
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179696-plasmid-map-sequence-id-352199

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  19. pFastBac HT JS-Rab10wt

    Plasmid
    #135557
    Purpose
    Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.
    Depositor
    Jingshi Shen
    Article
    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11
    Insert
    Rab10 (Rab10 Mouse)
    Tags
    6xHis tag, TEV site
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135557-plasmid-map-sequence-id-272501

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Showing: 81 - 100 of 643 results