172,536 results
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Plasmid#194703PurposeInducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)DepositorInsertRPS2 (RPS2 Human)
UseLentiviralTagsAPOBEC1-HA-P2A-mRubyExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tdTomato (codon diversified) (AAV Retrograde)
Viral Prep#59462-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. tdTomato expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (codon diversified)Available SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-eYFP (AAV Retrograde)
Viral Prep#117382-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNIL
Plasmid#172113PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expressionTagsT2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and TRE3GAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29a-human histone 4-in-One
Plasmid#223237PurposePolycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4DepositorInsertsHis6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII
TagsDual StrepII, His6, TEV protease site, and VSVGExpressionBacterialMutationA103GPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV/SB11
Plasmid#26552DepositorInsertSleeping Beauty
ExpressionBacterialMutationMethionine 243 to Glutamine Threonine 136 to Arg…Available SinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
3X FLAG-FUS-WT
Plasmid#44985DepositorAvailable SinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-C1
Plasmid#54653PurposeLocalization: C1 Cloning Vector, Excitation: 554, Emission: 581DepositorTypeEmpty backboneTagstdTomatoExpressionMammalianAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChrimsonR-tdT (AAV1)
Viral Prep#59171-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagstdTomatoAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn
Plasmid#174824PurposeMammalian expression of human MLH1dn (codon optimized)DepositorInserthuman MLH1dn (codon optimized)
ExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8s-WPRE (AAV1)
Viral Prep#162374-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacExpressionMammalianAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1
Plasmid#222339PurposePlasmid for expressing evolved and engineered Bxb1 in the mammalian cellsDepositorInsertCodon-optimized evolved and engineered Bxb1
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-mCherry
Plasmid#99154PurposeLentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.DepositorInsertsCas9
mCherry
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NSAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-His
Plasmid#218190PurposeBacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)DepositorArticleInsertnucA
TagsHis and NSP4 signal secretion peptideExpressionBacterialMutationcodon optimized ecoli residues - 22-266PromoterT7Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV Retrograde)
Viral Prep#37825-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-3xFLAG
Plasmid#123223PurposeN-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTags3xFLAGExpressionMammalianPromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV(CAT)T7-SB100
Plasmid#34879DepositorInsertSB100X transposase
ExpressionMammalianPromoterCMVAvailable SinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP
Plasmid#11680DepositorAvailable SinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only