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Plasmid#14437PurposeMammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.DepositorInsertRab 5 (RAB5A Human)
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10844
Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro
Plasmid#1764PurposeRetroviral vector for cloning and expressing your gene of interest. Puromycin selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-EGFP
Plasmid#122848PurposeN-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5)
Viral Prep#52925-AAV5PurposeReady-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) cyto-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA
Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertSPgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
mScarletI-Rab7
Plasmid#112955PurposeIn vivo visualization of Rab7 (can be used for colocalization studies)DepositorArticleInsertRab7
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-somBiPOLES-mCerulean (AAV9)
Viral Prep#154951-AAV9PurposeReady-to-use AAV9 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCerulean (Cre-dependent)Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseTagsExpressionBacterialMutationPromoternative promoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8m-WPRE
Plasmid#162375PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-dGBP1-TagBFP
Plasmid#80086PurposeGFP-dependent dGBP1-TagBFP, for expression in mammalian cellsDepositorInsertdGBP1-TagBFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSOUP
Plasmid#165419PurposeThe pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in AgrobacteriumInsertRep A
UseHelper plasmid required for replication for all p…TagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-RdLight1 (AAV9)
Viral Prep#125708-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin1-RdLight1 (#125708). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-RdLight1 plasmid DNA. Synapsin-driven expression of red genetically encoded dopamine sensor RdLight1. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cre
Plasmid#123133PurposeMammalian expression vector for wild-type Cre.DepositorInsertCre recombinase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-NanoLuc
Plasmid#87696PurposeGal4VP16 driven expression of NanoLucDepositorInsertNanoLuc
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV9-X1.1
Plasmid#196836Purposenon-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV9-X1.1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA CO Tol2 TPase
Plasmid#133032PurposeExpression vector for production of codon optimized Tol2 transposaseDepositorInsertCodon optimized Tol2 Transposase
UseIn vitro expressionTagsExpressionMutationCodon OptimizedPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-alphaTubulin_C1
Plasmid#189768PurposeConstruct for labelling microtubules in mammalian cells with mScarlet3-alphatubulinDepositorInsertmScarlet3-alphaTubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1_IVT
Plasmid#222342PurposePlasmid for evolved and engineered Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTemplate for in vitro transcriptionTagsExpressionMutationPromoterT7 (inactivated)Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mCherry (AAV8)
Viral Prep#114469-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only