pLKO.3G
(Plasmid #14748)

• Purpose: (Empty Backbone)
• Depositing Labs: Christophe Benoist, Diane Mathis
• Publication: pLKO variants (unpublished)
• Citations: 46 Articles

Backbone

• Vector backbone: pLKO.3G
• Backbone size (bp): 8174
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: eGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)
• Reference:
  • Addgene's pLKO.1 protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A variant of pLKO.1 containing an eGFP marker rather than puromycin. Please note that the AgeI site is NOT unique for this vector, so shRNAs should be designed for cloning with EcoRI and PacI instead. Forward oligo: 5'-AATT—21bp sense—CTCGAG—21bp antisense—TTTTTTTAT-3'. Reverse oligo: 5'-AAAAAAA—21bp sense—CTCGAG—21bp antisense-3'.

pLKO.3G was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with eGFP from another vector using the enzymes BamHI and BsRGI.