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Showing: 61 - 80 of 164890 results

  1. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV8)

    Viral Prep
    #44362-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. Cre Reporter

    Plasmid
    #62732
    Purpose
    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).
    Depositor
    Niels Geijsen
    Article
    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1
    Insert
    LoxP-DsRED-STOP-LoxP-eGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    LoxP sites
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-Alpha
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    62732-plasmid-map-sequence-id-204219

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  4. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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  10. Joint Modular Cloning (JMC) Toolkit

    Plasmid Kit
    #1000000233
    Purpose
    The Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional parts for assembling plant transformation vectors.
    Depositor
    Daniel Voytas
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only

  11. pFastBac HT JS-Munc18c

    Plasmid
    #135555
    Purpose
    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18c
    Use
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135555-plasmid-map-sequence-id-272498

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  13. INCTbiosyn-p35S(rc)2_4_5-GFP

    Plasmid
    #127520
    Purpose
    Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127520-plasmid-map-sequence-id-250826

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  14. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV5)

    Viral Prep
    #44362-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. AAV pCAG-FLEX-EGFP-WPRE (AAV9)

    Viral Prep
    #51502-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do
    Promoter
    CAG
    Tags
    EGFP (Cre-dependent)
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pCMV-PE6c

    Plasmid
    #207853
    Purpose
    Mammalian expression of PE6c prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6c
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207853-plasmid-map-sequence-id-423164

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Showing: 61 - 80 of 164890 results