202,311 results

1. pCMV-dR8.2 dvpr

   Plasmid

   #8455

   Purpose

   2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-dR8.2 dvpr

   Plasmid

   #8455

   Purpose

   2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRE112

   Plasmid

   #43828

   Depositor

   Dieter Schifferli

   Article

   Edwards et al Gene. 1998 Jan 30;207(2):149-57.

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb1

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRE112

   Plasmid

   #43828

   Depositor

   Dieter Schifferli

   Article

   Edwards et al Gene. 1998 Jan 30;207(2):149-57.

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb1

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3_mScarlet-LAMP1

   Plasmid

   #185138

   Purpose

   Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

   Depositor

   Andrew York

   Article

   Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

   Insert

   Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-21 of LAMP1

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Use

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2546

   Plasmid

   #144209

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CEBPA (CEBPA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-Mff

   Plasmid

   #49153

   Purpose

   mammalian expression of Mff fused to GFP

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   Mff (MFF Human)
   

   Use

   Tags

   acGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Mff

   Plasmid

   #49153

   Purpose

   mammalian expression of Mff fused to GFP

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   Mff (MFF Human)
   

   Use

   Tags

   acGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJQ200SK

    Plasmid

    #78497

    Purpose

    Suicide vector for gene replacement

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Plasmid

    #163076

    Purpose

    Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Plasmid

    #163075

    Purpose

    Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-NT*-HRV3CP

    Plasmid

    #162795

    Purpose

    Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

    Depositor

    Gottfried Otting

    Article

    Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

    Insert

    Human rhinovirus 14 3C protease
    

    Use

    Tags

    NT* solubility tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-loxp-DsRed-loxp-eGFP

    Plasmid

    #141148

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    loxp-DsRed-loxp-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330 p53

    Plasmid

    #59910

    Purpose

    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting p53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330 p53

    Plasmid

    #59910

    Purpose

    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting p53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSH424-ssr

    Plasmid

    #198026

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Sm resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SmR promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUW-tetO-hOKMS

    Plasmid

    #51543

    Purpose

    Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

    Depositor

    Tarjei Mikkelsen

    Article

    Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

    Insert

    hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUW-tetO-hOKMS

    Plasmid

    #51543

    Purpose

    Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

    Depositor

    Tarjei Mikkelsen

    Article

    Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

    Insert

    hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge