240,169 results

1. pUC57-mitoTPNOX

   Plasmid

   #87854

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   mitoTPNOX
   

   Tags

   C-terminal FLAG tag with a linker and mitochondri…

   Expression

   Bacterial

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-mitoTPNOX

   Plasmid

   #87854

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   mitoTPNOX
   

   Tags

   C-terminal FLAG tag with a linker and mitochondri…

   Expression

   Bacterial

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-Cre-P2A-dTomato

   Plasmid

   #107738

   Purpose

   Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.

   Depositor

   Rylan Larsen

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre, dTomato
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-Cre-P2A-dTomato

   Plasmid

   #107738

   Purpose

   Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.

   Depositor

   Rylan Larsen

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre, dTomato
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

   Plasmid

   #214885

   Purpose

   Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1-GfaABC1D-lck-jGCaMP8m (AAV1)

   Viral Prep

   #176760-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8m (#176760). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8m plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   GfaABC1D

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

7. pAdDeltaF6

   Plasmid

   #112867

   Purpose

   AAV helper plasmid

   Depositor

   James M. Wilson

   Insert

   E4, E2a and VA
   

   Use

   AAV

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAdDeltaF6

   Plasmid

   #112867

   Purpose

   AAV helper plasmid

   Depositor

   James M. Wilson

   Insert

   E4, E2a and VA
   

   Use

   AAV

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-mCherry (AAV Retrograde)

   Viral Prep

   #114472-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   Syn

   Tags

   mCherry

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pEVOL-pAzF

    Plasmid

    #31186

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

    Depositor

    Peter Schultz

    Article

    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

    Insert

    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    

    Expression

    Bacterial

    Mutation

    Y32T, E107N, D158P, I159L, L162Q, D286R

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEVOL-pAzF

    Plasmid

    #31186

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

    Depositor

    Peter Schultz

    Article

    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

    Insert

    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    

    Expression

    Bacterial

    Mutation

    Y32T, E107N, D158P, I159L, L162Q, D286R

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1

    Plasmid

    #41393

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCW57.1

    Plasmid

    #41393

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSV-Rev

    Plasmid

    #12253

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    Rev (rev )
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSV-Rev

    Plasmid

    #12253

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    Rev (rev )
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-tevopreq1-GG-acceptor

    Plasmid

    #174038

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2/5

    Plasmid

    #232922

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Rep2/Cap5
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Sept. 17, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV-hSyn-DIO-mCherry (AAV5)

    Viral Prep

    #50459-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

19. CRISPRoff-v2.1

    Plasmid

    #167981

    Purpose

    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

    Depositor

    Luke Gilbert

    Article

    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

    Insert

    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    

    Tags

    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV9)

    Viral Prep

    #44361-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only