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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) Citations (121)

Plasmid Article: Multiplex Genome Engineering Using CRISPR/Cas Systems.
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F Science. 2013 Jan 3.
PubMed Journal

Articles Citing pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

Articles
Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. PubMed

Associated Plasmids

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Wu X, Scott DA, Kriz AJ, Chiu AC, Hsu PD, Dadon DB, Cheng AW, Trevino AE, Konermann S, Chen S, Jaenisch R, Zhang F, Sharp PA. Nat Biotechnol. 2014 Jul;32(7):670-6. doi: 10.1038/nbt.2889. Epub 2014 Apr 20. PubMed
An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells. Munoz MC, Yanez DA, Stark JM. Nucleic Acids Res. 2014 Jul;42(12):7720-33. doi: 10.1093/nar/gku421. Epub 2014 May 14. PubMed
Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks. Renouf B, Piganeau M, Ghezraoui H, Jasin M, Brunet E. Methods Enzymol. 2014;546:251-71. doi: 10.1016/B978-0-12-801185-0.00012-X. PubMed
Genome engineering using CRISPR-Cas9 system. Cong L, Zhang F. Methods Mol Biol. 2015;1239:197-217. doi: 10.1007/978-1-4939-1862-1_10. PubMed
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Frock RL, Hu J, Meyers RM, Ho YJ, Kii E, Alt FW. Nat Biotechnol. 2015 Feb;33(2):179-86. doi: 10.1038/nbt.3101. Epub 2014 Dec 15. PubMed
Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing. Parikh BA, Beckman DL, Patel SJ, White JM, Yokoyama WM. PLoS One. 2015 Jan 14;10(1):e0116484. doi: 10.1371/journal.pone.0116484. eCollection 2015. PubMed
Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus. Merkle FT, Neuhausser WM, Santos D, Valen E, Gagnon JA, Maas K, Sandoe J, Schier AF, Eggan K. Cell Rep. 2015 May 12;11(6):875-83. doi: 10.1016/j.celrep.2015.04.007. Epub 2015 Apr 30. PubMed
Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system. Li S, Xue H, Long B, Sun L, Truong T, Liu Y. J Vis Exp. 2015 May 28;(99):e52539. doi: 10.3791/52539. PubMed
Simultaneous live imaging of the transcription and nuclear position of specific genes. Ochiai H, Sugawara T, Yamamoto T. Nucleic Acids Res. 2015 Jun 19. pii: gkv624. PubMed

Associated Plasmids

CRISPR-mediated genotypic and phenotypic correction of a chronic granulomatous disease mutation in human iPS cells. Flynn R, Grundmann A, Renz P, Hanseler W, James WS, Cowley SA, Moore MD. Exp Hematol. 2015 Oct;43(10):838-848.e3. doi: 10.1016/j.exphem.2015.06.002. Epub 2015 Jun 19. PubMed
CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells. Haraguchi M, Sato M, Ozawa M. PLoS One. 2015 Jul 10;10(7):e0132260. doi: 10.1371/journal.pone.0132260. eCollection 2015. PubMed
Nuclear domain 'knock-in' screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing. Pinder J, Salsman J, Dellaire G. Nucleic Acids Res. 2015 Oct 30;43(19):9379-92. doi: 10.1093/nar/gkv993. Epub 2015 Oct 1. PubMed

Associated Plasmids

Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1. Lai YC, Kondapalli C, Lehneck R, Procter JB, Dill BD, Woodroof HI, Gourlay R, Peggie M, Macartney TJ, Corti O, Corvol JC, Campbell DG, Itzen A, Trost M, Muqit MM. EMBO J. 2015 Nov 12;34(22):2840-61. doi: 10.15252/embj.201591593. Epub 2015 Oct 15. PubMed
Congenital Myasthenic Syndrome Type 19 Is Caused by Mutations in COL13A1, Encoding the Atypical Non-fibrillar Collagen Type XIII alpha1 Chain. Logan CV, Cossins J, Rodriguez Cruz PM, Parry DA, Maxwell S, Martinez-Martinez P, Riepsaame J, Abdelhamed ZA, Lake AV, Moran M, Robb S, Chow G, Sewry C, Hopkins PM, Sheridan E, Jayawant S, Palace J, Johnson CA, Beeson D. Am J Hum Genet. 2015 Dec 3;97(6):878-85. doi: 10.1016/j.ajhg.2015.10.017. Epub 2015 Nov 25. PubMed
Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. Wu J, Hunt SD, Xue H, Liu Y, Darabi R. Sci Rep. 2016 Jan 5;6:18759. doi: 10.1038/srep18759. PubMed
Generation and validation of PAX7 reporter lines from human iPS cells using CRISPR/Cas9 technology. Wu J, Hunt SD, Xue H, Liu Y, Darabi R. Stem Cell Res. 2016 Jan 13;16(2):220-228. doi: 10.1016/j.scr.2016.01.003. PubMed
Correction of the auditory phenotype in C57BL/6N mice via CRISPR/Cas9-mediated homology directed repair. Mianne J, Chessum L, Kumar S, Aguilar C, Codner G, Hutchison M, Parker A, Mallon AM, Wells S, Simon MM, Teboul L, Brown SD, Bowl MR. Genome Med. 2016 Feb 15;8(1):16. doi: 10.1186/s13073-016-0273-4. PubMed
Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases. Yang D, Scavuzzo MA, Chmielowiec J, Sharp R, Bajic A, Borowiak M. Sci Rep. 2016 Feb 18;6:21264. doi: 10.1038/srep21264. PubMed
Inactivation of prosurvival Bcl-2 proteins activates Bax/Bak through the outer mitochondrial membrane. O'Neill KL, Huang K, Zhang J, Chen Y, Luo X. Genes Dev. 2016 Apr 15;30(8):973-88. doi: 10.1101/gad.276725.115. Epub 2016 Apr 7. PubMed
Nipah Virus C Protein Recruits Tsg101 to Promote the Efficient Release of Virus in an ESCRT-Dependent Pathway. Park A, Yun T, Vigant F, Pernet O, Won ST, Dawes BE, Bartkowski W, Freiberg AN, Lee B. PLoS Pathog. 2016 May 20;12(5):e1005659. doi: 10.1371/journal.ppat.1005659. eCollection 2016 May. PubMed
Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Zhang J, Huang K, O'Neill KL, Pang X, Luo X. Cell Death Dis. 2016 Jun 16;7:e2266. doi: 10.1038/cddis.2016.167. PubMed
Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy. Heintze J, Costa JR, Weber M, Ketteler R. Cell Signal. 2016 Jun 18;28(9):1380-1388. doi: 10.1016/j.cellsig.2016.06.015. PubMed
Synthetic viability by BRCA2 and PARP1/ARTD1 deficiencies. Ding X, Ray Chaudhuri A, Callen E, Pang Y, Biswas K, Klarmann KD, Martin BK, Burkett S, Cleveland L, Stauffer S, Sullivan T, Dewan A, Marks H, Tubbs AT, Wong N, Buehler E, Akagi K, Martin SE, Keller JR, Nussenzweig A, Sharan SK. Nat Commun. 2016 Aug 8;7:12425. doi: 10.1038/ncomms12425. PubMed
Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours. Golias T, Papandreou I, Sun R, Kumar B, Brown NV, Swanson BJ, Pai R, Jaitin D, Le QT, Teknos TN, Denko NC. Sci Rep. 2016 Aug 8;6:31146. doi: 10.1038/srep31146. PubMed
Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Santos DP, Kiskinis E, Eggan K, Merkle FT. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15. PubMed
Enhancing a Wnt-Telomere Feedback Loop Restores Intestinal Stem Cell Function in a Human Organotypic Model of Dyskeratosis Congenita. Woo DH, Chen Q, Yang TL, Glineburg MR, Hoge C, Leu NA, Johnson FB, Lengner CJ. Cell Stem Cell. 2016 Sep 1;19(3):397-405. doi: 10.1016/j.stem.2016.05.024. Epub 2016 Aug 18. PubMed
The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells. Roncagalli R, Cucchetti M, Jarmuzynski N, Gregoire C, Bergot E, Audebert S, Baudelet E, Menoita MG, Joachim A, Durand S, Suchanek M, Fiore F, Zhang L, Liang Y, Camoin L, Malissen M, Malissen B. J Exp Med. 2016 Oct 17;213(11):2437-2457. Epub 2016 Sep 19. PubMed
An affinity-directed protein missile system for targeted proteolysis. Fulcher LJ, Macartney T, Bozatzi P, Hornberger A, Rojas-Fernandez A, Sapkota GP. Open Biol. 2016 Oct;6(10). pii: 160255. PubMed
Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression. Boehm E, Zornoza M, Jourdain AA, Delmiro Magdalena A, Garcia-Consuegra I, Torres Merino R, Orduna A, Martin MA, Martinou JC, De la Fuente MA, Simarro M. J Biol Chem. 2016 Dec 9;291(50):25877-25887. Epub 2016 Oct 27. PubMed
Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection. Perelman SS, Abrams ME, Eitson JL, Chen D, Jimenez A, Mettlen M, Schoggins JW, Alto NM. PLoS Pathog. 2016 Dec 21;12(12):e1006102. doi: 10.1371/journal.ppat.1006102. eCollection 2016 Dec. PubMed
CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Van Nostrand EL, Gelboin-Burkhart C, Wang R, Pratt GA, Blue SM, Yeo GW. Methods. 2016 Dec 18. pii: S1046-2023(16)30481-9. doi: 10.1016/j.ymeth.2016.12.007. PubMed
The Convenience of Single Homology Arm Donor DNA and CRISPR/Cas9-Nickase for Targeted Insertion of Long DNA Fragment. Basiri M, Behmanesh M, Tahamtani Y, Khalooghi K, Moradmand A, Baharvand H. Cell J. 2017 Winter;18(4):532-539. Epub 2016 Sep 26. PubMed
Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells. Sontag S, Forster M, Qin J, Wanek P, Mitzka S, Schuler HM, Koschmieder S, Rose-John S, Sere K, Zenke M. Stem Cells. 2017 Jan 16. doi: 10.1002/stem.2565. PubMed

Associated Plasmids

Genome Editing Reveals Glioblastoma Addiction to MicroRNA-10b. El Fatimy R, Subramanian S, Uhlmann EJ, Krichevsky AM. Mol Ther. 2017 Feb 1;25(2):368-378. doi: 10.1016/j.ymthe.2016.11.004. PubMed
Crosstalk between CLCb/Dyn1-Mediated Adaptive Clathrin-Mediated Endocytosis and Epidermal Growth Factor Receptor Signaling Increases Metastasis. Chen PH, Bendris N, Hsiao YJ, Reis CR, Mettlen M, Chen HY, Yu SL, Schmid SL. Dev Cell. 2017 Feb 6;40(3):278-288.e5. doi: 10.1016/j.devcel.2017.01.007. PubMed
Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9. Lv JN, Zhou GH, Chen X, Chen H, Wu KC, Xiang L, Lei XL, Zhang X, Wu RH, Jin ZB. Sci Rep. 2017 Feb 20;7:43062. doi: 10.1038/srep43062. PubMed
A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB. J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 10.1074/jbc.M117.777722. Epub 2017 Feb 22. PubMed

Associated Plasmids

CRISPR-Cas9 system-driven site-specific selection pressure on Herpes simplex virus genomes. Li Z, Bi Y, Xiao H, Sun L, Ren Y, Li Y, Chen C, Cun W. Virus Res. 2017 Mar 6. pii: S0168-1702(16)30694-3. doi: 10.1016/j.virusres.2017.03.010. PubMed
Regulation of membrane ruffling by polarized STIM1 and ORAI1 in cortactin-rich domains. Lopez-Guerrero AM, Tomas-Martin P, Pascual-Caro C, Macartney T, Rojas-Fernandez A, Ball G, Alessi DR, Pozo-Guisado E, Martin-Romero FJ. Sci Rep. 2017 Mar 24;7(1):383. doi: 10.1038/s41598-017-00331-4. PubMed
Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, Dekker J, Mirny LA, Bruneau BG. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. PubMed

Associated Plasmids

LC3-association with the parasitophorous vacuole membrane of Plasmodium berghei liver stages follows a noncanonical autophagy pathway. Wacker R, Eickel N, Schmuckli-Maurer J, Annoura T, Niklaus L, Khan SM, Guan JL, Heussler VT. Cell Microbiol. 2017 Jun 1. doi: 10.1111/cmi.12754. PubMed
Statins do not inhibit the FGFR signaling in chondrocytes. Fafilek B, Hampl M, Ricankova N, Vesela I, Balek L, Kunova Bosakova M, Gudernova I, Varecha M, Buchtova M, Krejci P. Osteoarthritis Cartilage. 2017 Jun 3. pii: S1063-4584(17)31024-5. doi: 10.1016/j.joca.2017.05.014. PubMed
Infection exposure promotes ETV6-RUNX1 precursor B cell leukemia via impaired H3K4 demethylases. Rodriguez-Hernandez G, Hauer J, Martin-Lorenzo A, Schafer D, Bartenhagen C, Garcia-Ramirez I, Auer F, Gonzalez-Herrero I, Ruiz-Roca L, Gombert M, Okpanyi V, Fischer U, Chen C, Dugas M, Bhatia S, Linka RM, Garcia-Suquia M, Rascon-Trincado MV, Garcia-Sanchez A, Blanco O, Garcia-Cenador MB, Garcia-Criado FJ, Cobaleda C, Alonso-Lopez D, De Las Rivas J, Muschen M, Vicente-Duenas C, Sanchez-Garcia I, Borkhardt A. Cancer Res. 2017 Jun 19. pii: canres.0701.2017. doi: 10.1158/0008-5472.CAN-17-0701. PubMed
Generation and cardiac subtype-specific differentiation of PITX2-deficient human iPS cell lines for exploring familial atrial fibrillation. Marczenke M, Fell J, Piccini I, Ropke A, Seebohm G, Greber B. Stem Cell Res. 2017 May;21:26-28. doi: 10.1016/j.scr.2017.03.015. Epub 2017 Mar 23. PubMed
A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid. Talas A, Kulcsar PI, Weinhardt N, Borsy A, Toth E, Szebenyi K, Krausz SL, Huszar K, Vida I, Sturm A, Gordos B, Hoffmann OI, Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E. DNA Res. 2017 Jun 30. doi: 10.1093/dnares/dsx029. PubMed

Associated Plasmids

Cardiac Subtype-Specific Modeling of Kv1.5 Ion Channel Deficiency Using Human Pluripotent Stem Cells. Marczenke M, Piccini I, Mengarelli I, Fell J, Ropke A, Seebohm G, Verkerk AO, Greber B. Front Physiol. 2017 Jul 6;8:469. doi: 10.3389/fphys.2017.00469. eCollection 2017. PubMed
Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo. Hanssen LLP, Kassouf MT, Oudelaar AM, Biggs D, Preece C, Downes DJ, Gosden M, Sharpe JA, Sloane-Stanley JA, Hughes JR, Davies B, Higgs DR. Nat Cell Biol. 2017 Aug;19(8):952-961. doi: 10.1038/ncb3573. Epub 2017 Jul 24. PubMed
Differential regulation of pro-inflammatory cytokine signalling by protein tyrosine phosphatases in pancreatic beta-cells. Stanley WJ, Trivedi PM, Sutherland AP, Thomas HE, Gurzov EN. J Mol Endocrinol. 2017 Nov;59(4):325-337. doi: 10.1530/JME-17-0089. Epub 2017 Aug 21. PubMed
Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane. Schmuckli-Maurer J, Reber V, Wacker R, Bindschedler A, Zakher A, Heussler VT. PLoS One. 2017 Aug 25;12(8):e0183797. doi: 10.1371/journal.pone.0183797. eCollection 2017. PubMed
Store-operated calcium entry is dispensable for the activation of ERK1/2 pathway in prostate cancer cells. Lopez-Guerrero AM, Pascual-Caro C, Martin-Romero FJ, Pozo-Guisado E. Cell Signal. 2017 Dec;40:44-52. doi: 10.1016/j.cellsig.2017.08.010. Epub 2017 Aug 31. PubMed
Loss of PRP4K drives anoikis resistance in part by dysregulation of epidermal growth factor receptor endosomal trafficking. Corkery DP, Clarke LE, Gebremeskel S, Salsman J, Pinder J, Le Page C, Meunier L, Xu Z, Mes-Masson AM, Berman JN, Johnston B, Dellaire G. Oncogene. 2018 Jan 11;37(2):174-184. doi: 10.1038/onc.2017.318. Epub 2017 Sep 11. PubMed
The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence. Mages CF, Wintsche A, Bernhart SH, Muller GA. Elife. 2017 Sep 18;6. doi: 10.7554/eLife.26876. PubMed
Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice. Miyauchi H, Ohta H, Nagaoka S, Nakaki F, Sasaki K, Hayashi K, Yabuta Y, Nakamura T, Yamamoto T, Saitou M. EMBO J. 2017 Nov 2;36(21):3100-3119. doi: 10.15252/embj.201796875. Epub 2017 Sep 19. PubMed
Adrenergic Stress Protection of Human iPS Cell-Derived Cardiomyocytes by Fast Kv7.1 Recycling. Piccini I, Fehrmann E, Frank S, Muller FU, Greber B, Seebohm G. Front Physiol. 2017 Sep 14;8:705. doi: 10.3389/fphys.2017.00705. eCollection 2017. PubMed
Evolutionarily Distinctive Transcriptional and Signaling Programs Drive Human Germ Cell Lineage Specification from Pluripotent Stem Cells. Kojima Y, Sasaki K, Yokobayashi S, Sakai Y, Nakamura T, Yabuta Y, Nakaki F, Nagaoka S, Woltjen K, Hotta A, Yamamoto T, Saitou M. Cell Stem Cell. 2017 Oct 5;21(4):517-532.e5. doi: 10.1016/j.stem.2017.09.005. PubMed
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. PubMed

Associated Plasmids

Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells. Schimmel J, Kool H, van Schendel R, Tijsterman M. EMBO J. 2017 Dec 15;36(24):3634-3649. doi: 10.15252/embj.201796948. Epub 2017 Oct 27. PubMed
Mechanism of activation of SGK3 by growth factors via the Class 1 and Class 3 PI3Ks. Malik N, Macartney T, Hornberger A, Anderson KE, Tovell H, Prescott AR, Alessi DR. Biochem J. 2018 Jan 2;475(1):117-135. doi: 10.1042/BCJ20170650. PubMed
Deletion of GIRK2 subunit containing GIRK channels of neurons expressing dopamine transporter decrease immobility time on forced swimming in mice. Honda I, Araki K, Honda S, Soeda F, Shin MC, Misumi S, Yamamura KI, Takahama K. Neurosci Lett. 2018 Feb 5;665:140-146. doi: 10.1016/j.neulet.2017.11.028. Epub 2017 Nov 24. PubMed
CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology. Wu WH, Tsai YT, Justus S, Cho GY, Sengillo JD, Xu Y, Cabral T, Lin CS, Bassuk AG, Mahajan VB, Tsang SH. Methods Mol Biol. 2018;1715:191-205. doi: 10.1007/978-1-4939-7522-8_13. PubMed
CRISPR/Cas9-mediated genome editing in naive human embryonic stem cells. Jacobs EZ, Warrier S, Volders PJ, D'haene E, Van Lombergen E, Vantomme L, Van der Jeught M, Heindryckx B, Menten B, Vergult S. Sci Rep. 2017 Nov 30;7(1):16650. doi: 10.1038/s41598-017-16932-y. PubMed
IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. Taulet N, Vitre B, Anguille C, Douanier A, Rocancourt M, Taschner M, Lorentzen E, Echard A, Delaval B. Nat Commun. 2017 Dec 4;8(1):1928. doi: 10.1038/s41467-017-01479-3. PubMed
Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. Adikusuma F, Pfitzner C, Thomas PQ. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. PubMed

Associated Plasmids

Reversible Conformational Conversion of alpha-Synuclein into Toxic Assemblies by Glucosylceramide. Zunke F, Moise AC, Belur NR, Gelyana E, Stojkovska I, Dzaferbegovic H, Toker NJ, Jeon S, Fredriksen K, Mazzulli JR. Neuron. 2017 Dec 20. pii: S0896-6273(17)31135-2. doi: 10.1016/j.neuron.2017.12.012. PubMed
Revised roles of ISL1 in a hES cell-based model of human heart chamber specification. Quaranta R, Fell J, Ruhle F, Rao J, Piccini I, Arauzo-Bravo MJ, Verkerk AO, Stoll M, Greber B. Elife. 2018 Jan 16;7. doi: 10.7554/eLife.31706. PubMed
Necessity of p53-binding to the CDH1 locus for its expression defines two epithelial cell types differing in their integrity. Oikawa T, Otsuka Y, Onodera Y, Horikawa M, Handa H, Hashimoto S, Suzuki Y, Sabe H. Sci Rep. 2018 Jan 25;8(1):1595. doi: 10.1038/s41598-018-20043-7. PubMed
Chromosomal instability during neurogenesis in Huntington's disease. Ruzo A, Croft GF, Metzger JJ, Galgoczi S, Gerber LJ, Pellegrini C, Wang H Jr, Fenner M, Tse S, Marks A, Nchako C, Brivanlou AH. Development. 2018 Jan 29;145(2). pii: 145/2/dev156844. doi: 10.1242/dev.156844. PubMed
Cardiogenic programming of human pluripotent stem cells by dose-controlled activation of EOMES. Pfeiffer MJ, Quaranta R, Piccini I, Fell J, Rao J, Ropke A, Seebohm G, Greber B. Nat Commun. 2018 Jan 30;9(1):440. doi: 10.1038/s41467-017-02812-6. PubMed
Insights into the ubiquitin-proteasome system of human embryonic stem cells. Saez I, Koyuncu S, Gutierrez-Garcia R, Dieterich C, Vilchez D. Sci Rep. 2018 Mar 6;8(1):4092. doi: 10.1038/s41598-018-22384-9. PubMed
Paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption. Gopalappa R, Suresh B, Ramakrishna S, Kim HH. Nucleic Acids Res. 2018 Jul 6;46(12):e71. doi: 10.1093/nar/gky222. PubMed
A Transcriptional Circuit Filters Oscillating Circadian Hormonal Inputs to Regulate Fat Cell Differentiation. Bahrami-Nejad Z, Zhao ML, Tholen S, Hunerdosse D, Tkach KE, van Schie S, Chung M, Teruel MN. Cell Metab. 2018 Apr 3;27(4):854-868.e8. doi: 10.1016/j.cmet.2018.03.012. PubMed
IKK1/2 protect human cells from TNF-mediated RIPK1-dependent apoptosis in an NF-kappaB-independent manner. Slotta C, Storm J, Pfisterer N, Henkel E, Kleinwachter S, Pieper M, Ruiz-Perera LM, Greiner JFW, Kaltschmidt B, Kaltschmidt C. Biochim Biophys Acta Mol Cell Res. 2018 Aug;1865(8):1025-1033. doi: 10.1016/j.bbamcr.2018.04.003. Epub 2018 Apr 7. PubMed
Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription. Butepage M, Preisinger C, von Kriegsheim A, Scheufen A, Lausberg E, Li J, Kappes F, Feederle R, Ernst S, Eckei L, Krieg S, Muller-Newen G, Rossetti G, Feijs KLH, Verheugd P, Luscher B. Sci Rep. 2018 Apr 30;8(1):6748. doi: 10.1038/s41598-018-25137-w. PubMed
The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human. Mir R, Tonelli F, Lis P, Macartney T, Polinski NK, Martinez TN, Chou MY, Howden AJM, Konig T, Hotzy C, Milenkovic I, Brucke T, Zimprich A, Sammler E, Alessi DR. Biochem J. 2018 Jun 6;475(11):1861-1883. doi: 10.1042/BCJ20180248. PubMed
Self-organization of a human organizer by combined Wnt and Nodal signalling. Martyn I, Kanno TY, Ruzo A, Siggia ED, Brivanlou AH. Nature. 2018 Jun;558(7708):132-135. doi: 10.1038/s41586-018-0150-y. Epub 2018 May 23. PubMed
Generation and validation of homozygous fluorescent knock-in cells using CRISPR-Cas9 genome editing. Koch B, Nijmeijer B, Kueblbeck M, Cai Y, Walther N, Ellenberg J. Nat Protoc. 2018 Jun;13(6):1465-1487. doi: 10.1038/nprot.2018.042. Epub 2018 May 24. PubMed
Efficient genome editing by FACS enrichment of paired D10A Cas9 nickases coupled with fluorescent proteins. Gopalappa R, Song M, Chandrasekaran AP, Das S, Haq S, Koh HC, Ramakrishna S. Arch Pharm Res. 2018 Sep;41(9):911-920. doi: 10.1007/s12272-018-1042-2. Epub 2018 May 31. PubMed
Optimized Plasmid Construction Strategy for Cas9. Xu J, Li W, Hossen MM, Jia Y, Li L, Huang Z. Cell Physiol Biochem. 2018;48(1):131-137. doi: 10.1159/000491669. Epub 2018 Jul 12. PubMed
Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2. Sevim H, Kocaefe YC, Onur MA, Uckan-Cetinkaya D, Gurpinar OA. Stem Cell Res Ther. 2018 Jul 18;9(1):198. doi: 10.1186/s13287-018-0941-y. PubMed
Heterotetrameric annexin A2/S100A10 (A2t) is essential for oncogenic human papillomavirus trafficking and capsid disassembly, and protects virions from lysosomal degradation. Taylor JR, Fernandez DJ, Thornton SM, Skeate JG, Luhen KP, Da Silva DM, Langen R, Kast WM. Sci Rep. 2018 Aug 3;8(1):11642. doi: 10.1038/s41598-018-30051-2. PubMed
A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3. Tajan M, Hock AK, Blagih J, Robertson NA, Labuschagne CF, Kruiswijk F, Humpton TJ, Adams PD, Vousden KH. Cell Metab. 2018 Nov 6;28(5):721-736.e6. doi: 10.1016/j.cmet.2018.07.005. Epub 2018 Aug 16. PubMed
AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A. Davies AK, Itzhak DN, Edgar JR, Archuleta TL, Hirst J, Jackson LP, Robinson MS, Borner GHH. Nat Commun. 2018 Sep 27;9(1):3958. doi: 10.1038/s41467-018-06172-7. PubMed
The ectodomains determine ligand function in vivo and selectivity of DLL1 and DLL4 toward NOTCH1 and NOTCH2 in vitro. Tveriakhina L, Schuster-Gossler K, Jarrett SM, Andrawes MB, Rohrbach M, Blacklow SC, Gossler A. Elife. 2018 Oct 5;7. pii: 40045. doi: 10.7554/eLife.40045. PubMed
Paired CRISPR/Cas9 Nickases Mediate Efficient Site-Specific Integration of F9 into rDNA Locus of Mouse ESCs. Wang Y, Zhao J, Duan N, Liu W, Zhang Y, Zhou M, Hu Z, Feng M, Liu X, Wu L, Li Z, Liang D. Int J Mol Sci. 2018 Oct 5;19(10). pii: ijms19103035. doi: 10.3390/ijms19103035. PubMed
Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method. Sato T, Ogawa T. Methods Mol Biol. 2019;1874:87-98. doi: 10.1007/978-1-4939-8831-0_5. PubMed
Generation and characterization of pathogenic Mab21l2(R51C) mouse model. Tsang SW, Guo Y, Chan LH, Huang Y, Chow KL. Genesis. 2018 Dec;56(11-12):e23261. doi: 10.1002/dvg.23261. Epub 2018 Nov 29. PubMed
Generation of Endogenous BMP Transcriptional Reporter Cells Through CRISPR/Cas9 Genome Editing. Hutchinson LD, Bozatzi P, Macartney T, Sapkota GP. Methods Mol Biol. 2019;1891:29-35. doi: 10.1007/978-1-4939-8904-1_4. PubMed
A B-Cell-Specific Enhancer Orchestrates Nuclear Architecture to Generate a Diverse Antigen Receptor Repertoire. Barajas-Mora EM, Kleiman E, Xu J, Carrico NC, Lu H, Oltz EM, Murre C, Feeney AJ. Mol Cell. 2019 Jan 3;73(1):48-60.e5. doi: 10.1016/j.molcel.2018.10.013. Epub 2018 Nov 15. PubMed
Neural circuitry of a polycystin-mediated hydrodynamic startle response for predator avoidance. Bezares-Calderon LA, Berger J, Jasek S, Veraszto C, Mendes S, Guhmann M, Almeda R, Shahidi R, Jekely G. Elife. 2018 Dec 14;7. pii: 36262. doi: 10.7554/eLife.36262. PubMed
Optimizing genome editing strategy by primer-extension-mediated sequencing. Yin J, Liu M, Liu Y, Wu J, Gan T, Zhang W, Li Y, Zhou Y, Hu J. Cell Discov. 2019 Mar 26;5:18. doi: 10.1038/s41421-019-0088-8. eCollection 2019. PubMed
DSB structure impacts DNA recombination leading to class switching and chromosomal translocations in human B cells. So CC, Martin A. PLoS Genet. 2019 Apr 4;15(4):e1008101. doi: 10.1371/journal.pgen.1008101. eCollection 2019 Apr. PubMed
Rapid and Reversible Knockdown of Endogenously Tagged Endosomal Proteins via an Optimized HaloPROTAC Degrader. Tovell H, Testa A, Maniaci C, Zhou H, Prescott AR, Macartney T, Ciulli A, Alessi DR. ACS Chem Biol. 2019 May 17;14(5):882-892. doi: 10.1021/acschembio.8b01016. Epub 2019 Apr 22. PubMed
Ribozyme-mediated, multiplex CRISPR gene editing and CRISPR interference (CRISPRi) in rodent-infectious Plasmodium yoelii. Walker MP, Lindner SE. J Biol Chem. 2019 Jun 14;294(24):9555-9566. doi: 10.1074/jbc.RA118.007121. Epub 2019 May 1. PubMed

Associated Plasmids

Developing a CRISPR/Cas9 System for Genome Editing in the Basidiomycetous Yeast Rhodosporidium toruloides. Jiao X, Zhang Y, Liu X, Zhang Q, Zhang S, Zhao ZK. Biotechnol J. 2019 Jul;14(7):e1900036. doi: 10.1002/biot.201900036. Epub 2019 Jun 3. PubMed
Comprehensive substrate specificity profiling of the human Nek kinome reveals unexpected signaling outputs. van de Kooij B, Creixell P, van Vlimmeren A, Joughin BA, Miller CJ, Haider N, Simpson CD, Linding R, Stambolic V, Turk BE, Yaffe MB. Elife. 2019 May 24;8. pii: 44635. doi: 10.7554/eLife.44635. PubMed
The bipartite TAD organization of the X-inactivation center ensures opposing developmental regulation of Tsix and Xist. van Bemmel JG, Galupa R, Gard C, Servant N, Picard C, Davies J, Szempruch AJ, Zhan Y, Zylicz JJ, Nora EP, Lameiras S, de Wit E, Gentien D, Baulande S, Giorgetti L, Guttman M, Hughes JR, Higgs DR, Gribnau J, Heard E. Nat Genet. 2019 Jun;51(6):1024-1034. doi: 10.1038/s41588-019-0412-0. Epub 2019 May 27. PubMed
The inositol hexakisphosphate kinases IP6K1 and -2 regulate human cellular phosphate homeostasis, including XPR1-mediated phosphate export. Wilson MS, Jessen HJ, Saiardi A. J Biol Chem. 2019 Jul 26;294(30):11597-11608. doi: 10.1074/jbc.RA119.007848. Epub 2019 Jun 11. PubMed
Absolute quantification of cohesin, CTCF and their regulators in human cells. Holzmann J, Politi AZ, Nagasaka K, Hantsche-Grininger M, Walther N, Koch B, Fuchs J, Durnberger G, Tang W, Ladurner R, Stocsits RR, Busslinger GA, Novak B, Mechtler K, Davidson IF, Ellenberg J, Peters JM. Elife. 2019 Jun 17;8. pii: 46269. doi: 10.7554/eLife.46269. PubMed
Disease modeling of core pre-mRNA splicing factor haploinsufficiency. Wood KA, Rowlands CF, Qureshi WMS, Thomas HB, Buczek WA, Briggs TA, Hubbard SJ, Hentges KE, Newman WG, O'Keefe RT. Hum Mol Genet. 2019 Nov 15;28(22):3704-3723. doi: 10.1093/hmg/ddz169. PubMed
Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice. Kaczmarczyk L, Bansal V, Rajput A, Rahman RU, Krzyzak W, Degen J, Poll S, Fuhrmann M, Bonn S, Jackson WS. PLoS Biol. 2019 Aug 8;17(8):e3000374. doi: 10.1371/journal.pbio.3000374. eCollection 2019 Aug. PubMed
CRISPR/Cas9-mediated Knockout of the Neuropsychiatric Risk Gene KCTD13 Causes Developmental Deficits in Human Cortical Neurons Derived from Induced Pluripotent Stem Cells. Kizner V, Naujock M, Fischer S, Jager S, Reich S, Schlotthauer I, Zuckschwerdt K, Geiger T, Hildebrandt T, Lawless N, Macartney T, Dorner-Ciossek C, Gillardon F. Mol Neurobiol. 2020 Feb;57(2):616-634. doi: 10.1007/s12035-019-01727-1. Epub 2019 Aug 11. PubMed
The lncRNA Locus Handsdown Regulates Cardiac Gene Programs and Is Essential for Early Mouse Development. Ritter N, Ali T, Kopitchinski N, Schuster P, Beisaw A, Hendrix DA, Schulz MH, Muller-McNicoll M, Dimmeler S, Grote P. Dev Cell. 2019 Sep 9;50(5):644-657.e8. doi: 10.1016/j.devcel.2019.07.013. Epub 2019 Aug 15. PubMed
SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Panier S, Maric M, Hewitt G, Mason-Osann E, Gali H, Dai A, Labadorf A, Guervilly JH, Ruis P, Segura-Bayona S, Belan O, Marzec P, Gaillard PHL, Flynn RL, Boulton SJ. Mol Cell. 2019 Oct 3;76(1):27-43.e11. doi: 10.1016/j.molcel.2019.07.010. Epub 2019 Aug 22. PubMed
Endogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions. Strebinger D, Deluz C, Friman ET, Govindan S, Alber AB, Suter DM. Mol Syst Biol. 2019 Sep;15(9):e9002. doi: 10.15252/msb.20199002. PubMed
Stabilization of chromatin topology safeguards genome integrity. Ochs F, Karemore G, Miron E, Brown J, Sedlackova H, Rask MB, Lampe M, Buckle V, Schermelleh L, Lukas J, Lukas C. Nature. 2019 Oct;574(7779):571-574. doi: 10.1038/s41586-019-1659-4. Epub 2019 Oct 23. PubMed
MicroRNA-421 confers paclitaxel resistance by binding to the KEAP1 3'UTR and predicts poor survival in non-small cell lung cancer. Duan FG, Wang MF, Cao YB, Dan Li, Li RZ, Fan XX, Khan I, Lai HL, Zhang YZ, Hsiao WW, Yao XJ, Wu QB, Liu L, Tang YJ, Leung EL. Cell Death Dis. 2019 Oct 28;10(11):821. doi: 10.1038/s41419-019-2031-1. PubMed
PPM1H phosphatase counteracts LRRK2 signaling by selectively dephosphorylating Rab proteins. Berndsen K, Lis P, Yeshaw WM, Wawro PS, Nirujogi RS, Wightman M, Macartney T, Dorward M, Knebel A, Tonelli F, Pfeffer SR, Alessi DR. Elife. 2019 Oct 30;8. pii: 50416. doi: 10.7554/eLife.50416. PubMed
Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. Malik N, Nirujogi RS, Peltier J, Macartney T, Wightman M, Prescott AR, Gourlay R, Trost M, Alessi DR, Karapetsas A. Biochem J. 2019 Oct 30;476(20):3081-3107. doi: 10.1042/BCJ20190608. PubMed
PML isoform expression and DNA break location relative to PML nuclear bodies impacts the efficiency of homologous recombination. Attwood KM, Salsman J, Chung D, Mathavarajah S, Van Iderstine C, Dellaire G. Biochem Cell Biol. 2019 Oct 31. doi: 10.1139/bcb-2019-0115. PubMed
Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4. Liang J, Wang N, He J, Du J, Guo Y, Li L, Wu W, Yao C, Li Z, Kee K. Elife. 2019 Nov 11;8. pii: 48767. doi: 10.7554/eLife.48767. PubMed
Induction of the Germ-Cell Fate from Pluripotent Stem Cells in Cynomolgus Monkeys. Sakai Y, Nakamura T, Okamoto I, Gyobu-Motani S, Ohta H, Yabuta Y, Tsukiyama T, Iwatani C, Tsuchiya H, Ema M, Morizane A, Takahashi J, Yamamoto T, Saitou M. Biol Reprod. 2019 Nov 13. pii: 5625171. doi: 10.1093/biolre/ioz205. PubMed
Congenital myasthenic syndrome due to mutations in MUSK suggests that the level of MuSK phosphorylation is crucial for governing synaptic structure. Rodriguez Cruz PM, Cossins J, Cheung J, Maxwell S, Jayawant S, Herbst R, Waithe D, Kornev AP, Palace J, Beeson D. Hum Mutat. 2020 Mar;41(3):619-631. doi: 10.1002/humu.23949. Epub 2019 Nov 25. PubMed
The Oncogenic Kaposi's Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA Family. Morrison K, Manzano M, Chung K, Schipma MJ, Bartom ET, Gottwein E. Cell Rep. 2019 Dec 3;29(10):2961-2969.e6. doi: 10.1016/j.celrep.2019.11.005. PubMed
miR20b promotes growth of nonsmall cell lung cancer through a positive feedback loop of the Wnt/betacatenin signaling pathway. Ren T, Fan XX, Wang MF, Duan FG, Wei CL, Li RZ, Jiang ZB, Wang YW, Yao XJ, Chen MW, Tang YJ, Leung EL. Int J Oncol. 2020 Feb;56(2):470-479. doi: 10.3892/ijo.2019.4940. Epub 2019 Dec 13. PubMed
Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9. Huo Z, Tu J, Lee DF, Zhao R. Methods Mol Biol. 2020;2108:355-369. doi: 10.1007/978-1-0716-0247-8_29. PubMed
ELL-associated factors EAF1/2 negatively regulate HIV-1 transcription through inhibition of Super Elongation Complex formation. Liu R, Chen C, Li Y, Huang Q, Xue Y. Biochim Biophys Acta Gene Regul Mech. 2020 May;1863(5):194508. doi: 10.1016/j.bbagrm.2020.194508. Epub 2020 Feb 19. PubMed
FAM111A protects replication forks from protein obstacles via its trypsin-like domain. Kojima Y, Machida Y, Palani S, Caulfield TR, Radisky ES, Kaufmann SH, Machida YJ. Nat Commun. 2020 Mar 12;11(1):1318. doi: 10.1038/s41467-020-15170-7. PubMed
RAC1-Dependent ORAI1 Translocation to the Leading Edge Supports Lamellipodia Formation and Directional Persistence. Lopez-Guerrero AM, Espinosa-Bermejo N, Sanchez-Lopez I, Macartney T, Pascual-Caro C, Orantos-Aguilera Y, Rodriguez-Ruiz L, Perez-Oliva AB, Mulero V, Pozo-Guisado E, Martin-Romero FJ. Sci Rep. 2020 Apr 20;10(1):6580. doi: 10.1038/s41598-020-63353-5. PubMed
EZH2-Deficient T-cell Acute Lymphoblastic Leukemia Is Sensitized to CHK1 Inhibition through Enhanced Replication Stress. Leon TE, Rapoz-D'Silva T, Bertoli C, Rahman S, Magnussen M, Philip B, Farah N, Richardson SE, Ahrabi S, Guerra-Assuncao JA, Gupta R, Nacheva EP, Henderson S, Herrero J, Linch DC, de Bruin RAM, Mansour MR. Cancer Discov. 2020 Jul;10(7):998-1017. doi: 10.1158/2159-8290.CD-19-0789. Epub 2020 Apr 29. PubMed
Design and Derivation of Multi-Reporter Pluripotent Stem Cell Lines via CRISPR/Cas9n-Mediated Homology-Directed Repair. Dettmer R, Naujok O. Curr Protoc Stem Cell Biol. 2020 Sep;54(1):e116. doi: 10.1002/cpsc.116. PubMed

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