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Multiplex Genome Engineering Using CRISPR/Cas Systems.
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F
Science. 2013 Jan 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
41863pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherryThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.
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42229pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puroThis plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.
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42230pX330-U6-Chimeric_BB-CBh-hSpCas9A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.
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42333pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puroThis plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.
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42335pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
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42337pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.
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