pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
(Plasmid #41863)

• Purpose: This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.
• Depositing Lab: Feng Zhang
• Publication: Cong et al Science. 2013 Jan 3.

Backbone

• Vector backbone: pLenti
• Backbone size w/o insert (bp): 7732
• Total vector size (bp): 13793
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: U6-forward
• 3′ sequencing primer: WPRE-Reverse

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function.

If you ordered or downloaded sequence of this vector prior to June 17, 2013, please note that while the plasmid you received is correct, the earlier version of vector sequence provided for this construct misses a small portion (~2kb) outside the insert, and we have now updated it with a complete version.

For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/