PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41863||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7732
- Total vector size (bp) 13793
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site None (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer U6-forward
- 3′ sequencing primer WPRE-Reverse (Common Sequencing Primers)
Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function.
If you ordered or downloaded sequence of this vector prior to June 17, 2013, please note that while the plasmid you received is correct, the earlier version of vector sequence provided for this construct misses a small portion (~2kb) outside the insert, and we have now updated it with a complete version.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry was a gift from Feng Zhang (Addgene plasmid # 41863)
For your References section:Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan 3. 10.1126/science.1231143 PubMed 23287718