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Blair Madison Lab: A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair.
Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB
J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 10.1074/jbc.M117.777722. Epub 2017 Feb 22.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
133385BII-CPspaH2BPiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)
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133386BII-C3HPiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)
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133387BII-C3H2BPiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)
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133388BII-C3HTKPiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)
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133389BII-B3HTKPiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)
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133390pBS-PtH2BPiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)
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133391pBS-PtHPiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)
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133392pBS-BtHPiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)
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