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Ribozyme-mediated, multiplex CRISPR gene editing and CRISPR interference (CRISPRi) in rodent-infectious Plasmodium yoelii.
Walker MP, Lindner SE
J Biol Chem. 2019 Jun 14;294(24):9555-9566. doi: 10.1074/jbc.RA118.007121. Epub 2019 May 1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
129520pSL1166pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation
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129521pSL1211pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation
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129522pSL1394pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation
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129523pSL1433pDEF-SpCas9::GFP, Empty Vector for RGR and HDR Template, PyBiP-minimal promoter, 3rd Generation
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129525pSL1045pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation
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129526pSL1103pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation
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129527pSL1315pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation
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