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Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
PubMed

Plasmids from Article

ID Plasmid  
92102pX330-Flag-SpCas9-HF1 (without sgRNA)
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92110HeFm1SpCas9
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92111HeFm2SpCas9
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92112pX330-HA-dSpCas9
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92113pX330-Flag-dSpCas9
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92114pX330-Flag-deSpCas9
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92115pX330-Flag-dSpCas9-HF1
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92116pX330-Flag-dHeFSpCas9
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92117pET-deSpCas9-VP64-6xHis
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92118pET-dSpCas9-HF1-VP64-6xHis
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92119pET-dHeFSpCas9-VP64-6xHis
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92120U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1
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92353pX330-Flag-wtSpCas9 (without sgRNA)
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92354pX330-Flag-eSpCas9 (without sgRNA)
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92355HeFSpCas9
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