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(Plasmid #92117)


Item Catalog # Description Quantity Price (USD)
Plasmid 92117 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    dead/inactive eSpCas9-NLS-3xFLAG-VP64
  • Alt name
    dead/inactive eSpCas9 (1.1)-NLS-3xFLAG-VP64
  • Species
    S. pyogenes
  • Insert Size (bp)
  • Mutation
    D10A, H840A, K848A, K1003A, R1060A
  • Promoter T7
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3xFLAG (C terminal on insert)
    • VP64 (C terminal on insert)
    • 6xHis (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Alternate plasmid name: pT7-dead eSpCas9 (1.1)-NLS-3xFLAG-VP64-6xHis (without U6-sgRNA coding sequence).

For detailed information and plasmid usage, please see the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-deSpCas9-VP64-6xHis was a gift from Ervin Welker (Addgene plasmid # 92117 ; ; RRID:Addgene_92117)
  • For your References section:

    Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. 10.1186/s13059-017-1318-8 [pii] PubMed 28985763