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Simultaneous live imaging of the transcription and nuclear position of specific genes.
Ochiai H, Sugawara T, Yamamoto T
Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
62345pBSKΔB-CAG-MCP-tdiRFP670-IRES-NeoExpresses MCP-tdiRFP670 in mammalian cells
62346pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-NeoExpresses rtTA2sM2 and tTSkid in mammalian cells
62347pBSKΔB-TRE-dCas9-EGFPPlasmid for tet-inducible expression of dCas9-EGFP in mammalian cells
62348pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFPAn empty optimized gRNA expression vector
62351pTV-Oct4-TK-HMSMouse Oct4 targeting vector