pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
(Plasmid #62348)

• Purpose: An empty optimized gRNA expression vector
• Depositing Lab: Hiroshi Ochiai
• Publication: Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv624.

Backbone

• Vector backbone: pBluescript
• Total vector size (bp): 8122
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: none
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (not destroyed)
• 3′ cloning site: BbsI (not destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Supplemental Documents:
  • GenBank file for pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
• A portion of this plasmid was derived from a plasmid made by: To construct this vector, pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was modified. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was obtained from Addgene which distributes it on behalf of Wellcome Trust Sanger Institute.
• Articles Citing this Plasmid:
  • 18 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry