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A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid.
Talas A, Kulcsar PI, Weinhardt N, Borsy A, Toth E, Szebenyi K, Krausz SL, Huszar K, Vida I, Sturm A, Gordos B, Hoffmann OI, Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E
DNA Res. 2017 Jun 30. doi: 10.1093/dnares/dsx029.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
80436pSc1Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
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80437pSc2Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
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80438pSc1-puroExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin
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80439pSc1-DDExpresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
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