A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid.
Talas A, Kulcsar PI, Weinhardt N, Borsy A, Toth E, Szebenyi K, Krausz SL, Huszar K, Vida I, Sturm A, Gordos B, Hoffmann OI, Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E
DNA Res. 2017 Jun 30. doi: 10.1093/dnares/dsx029.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80436 | pSc1 | Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell |
| 80437 | pSc2 | Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell |
| 80438 | pSc1-puro | Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin |
| 80439 | pSc1-DD | Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell |