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pSpCas9(BB)-2A-Puro (PX459) Citations (407)

Plasmid Article: Genome engineering using the CRISPR-Cas9 system.
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
PubMed Journal

Articles Citing pSpCas9(BB)-2A-Puro (PX459)

Articles
Intracellular alpha-ketoglutarate maintains the pluripotency of embryonic stem cells. Carey BW, Finley LW, Cross JR, Allis CD, Thompson CB. Nature. 2015 Feb 19;518(7539):413-6. doi: 10.1038/nature13981. Epub 2014 Dec 10. PubMed
Generation of genomic deletions in mammalian cell lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J Vis Exp. 2014 Jan 3;(95). doi: 10.3791/52118. PubMed
Fatty acid remodeling by LPCAT3 enriches arachidonate in phospholipid membranes and regulates triglyceride transport. Hashidate-Yoshida T, Harayama T, Hishikawa D, Morimoto R, Hamano F, Tokuoka SM, Eto M, Tamura-Nakano M, Yanobu-Takanashi R, Mukumoto Y, Kiyonari H, Okamura T, Kita Y, Shindou H, Shimizu T. Elife. 2015 Apr 21;4. doi: 10.7554/eLife.06328. PubMed
A YAP/TAZ-induced feedback mechanism regulates Hippo pathway homeostasis. Moroishi T, Park HW, Qin B, Chen Q, Meng Z, Plouffe SW, Taniguchi K, Yu FX, Karin M, Pan D, Guan KL. Genes Dev. 2015 Jun 15;29(12):1271-84. doi: 10.1101/gad.262816.115. PubMed
Xist Exon 7 Contributes to the Stable Localization of Xist RNA on the Inactive X-Chromosome. Yamada N, Hasegawa Y, Yue M, Hamada T, Nakagawa S, Ogawa Y. PLoS Genet. 2015 Aug 5;11(8):e1005430. doi: 10.1371/journal.pgen.1005430. eCollection 2015 Aug. PubMed
Alternative Wnt Signaling Activates YAP/TAZ. Park HW, Kim YC, Yu B, Moroishi T, Mo JS, Plouffe SW, Meng Z, Lin KC, Yu FX, Alexander CM, Wang CY, Guan KL. Cell. 2015 Aug 13;162(4):780-94. doi: 10.1016/j.cell.2015.07.013. PubMed
CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting. Courtney DG, Moore JE, Atkinson SD, Maurizi E, Allen EH, Pedrioli DM, McLean WH, Nesbit MA, Moore CB. Gene Ther. 2016 Jan;23(1):108-12. doi: 10.1038/gt.2015.82. Epub 2015 Aug 20. PubMed
Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca(2+) influx. Jing J, He L, Sun A, Quintana A, Ding Y, Ma G, Tan P, Liang X, Zheng X, Chen L, Shi X, Zhang SL, Zhong L, Huang Y, Dong MQ, Walker CL, Hogan PG, Wang Y, Zhou Y. Nat Cell Biol. 2015 Oct;17(10):1339-47. doi: 10.1038/ncb3234. Epub 2015 Aug 31. PubMed

Associated Plasmids

Nanopatterned Human iPSC-based Model of a Dystrophin-Null Cardiomyopathic Phenotype. Macadangdang J, Guan X, Smith AS, Lucero R, Czerniecki S, Childers MK, Mack DL, Kim DH. Cell Mol Bioeng. 2015 Sep;8(3):320-332. PubMed
Feedback Mechanisms Regulate Ets Variant 2 (Etv2) Gene Expression and Hematoendothelial Lineages. Koyano-Nakagawa N, Shi X, Rasmussen TL, Das S, Walter CA, Garry DJ. J Biol Chem. 2015 Nov 20;290(47):28107-19. doi: 10.1074/jbc.M115.662197. Epub 2015 Sep 22. PubMed
Inhibition of beta-catenin-TCF1 interaction delays differentiation of mouse embryonic stem cells. Chatterjee SS, Saj A, Gocha T, Murphy M, Gonsalves FC, Zhang X, Hayward P, Akgol Oksuz B, Shen SS, Madar A, Martinez Arias A, DasGupta R. J Cell Biol. 2015 Oct 12;211(1):39-51. doi: 10.1083/jcb.201503017. PubMed
CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in neurodevelopment. Wang P, Lin M, Pedrosa E, Hrabovsky A, Zhang Z, Guo W, Lachman HM, Zheng D. Mol Autism. 2015 Oct 19;6:55. doi: 10.1186/s13229-015-0048-6. eCollection 2015. PubMed
CTCF Binding Polarity Determines Chromatin Looping. de Wit E, Vos ES, Holwerda SJ, Valdes-Quezada C, Verstegen MJ, Teunissen H, Splinter E, Wijchers PJ, Krijger PH, de Laat W. Mol Cell. 2015 Nov 19;60(4):676-84. doi: 10.1016/j.molcel.2015.09.023. Epub 2015 Oct 29. PubMed
Blocking the association of HDAC4 with MAP1S accelerates autophagy clearance of mutant Huntingtin. Yue F, Li W, Zou J, Chen Q, Xu G, Huang H, Xu Z, Zhang S, Gallinari P, Wang F, McKeehan WL, Liu L. Aging (Albany NY). 2015 Oct;7(10):839-53. PubMed
The primate-specific noncoding RNA HPAT5 regulates pluripotency during human preimplantation development and nuclear reprogramming. Durruthy-Durruthy J, Sebastiano V, Wossidlo M, Cepeda D, Cui J, Grow EJ, Davila J, Mall M, Wong WH, Wysocka J, Au KF, Reijo Pera RA. Nat Genet. 2016 Jan;48(1):44-52. doi: 10.1038/ng.3449. Epub 2015 Nov 23. PubMed
The Hippo pathway effectors YAP and TAZ promote cell growth by modulating amino acid signaling to mTORC1. Hansen CG, Ng YL, Lam WL, Plouffe SW, Guan KL. Cell Res. 2015 Dec;25(12):1299-313. doi: 10.1038/cr.2015.140. Epub 2015 Nov 27. PubMed
FGF2 mediates hepatic progenitor cell formation during human pluripotent stem cell differentiation by inducing the WNT antagonist NKD1. Twaroski K, Mallanna SK, Jing R, DiFurio F, Urick A, Duncan SA. Genes Dev. 2015 Dec 1;29(23):2463-74. doi: 10.1101/gad.268961.115. PubMed
Reduced cholesterol levels impair Smoothened activation in Smith-Lemli-Opitz syndrome. Blassberg R, Macrae JI, Briscoe J, Jacob J. Hum Mol Genet. 2015 Dec 18. pii: ddv507. PubMed
Aurora A phosphorylation of WD40-repeat protein 62 in mitotic spindle regulation. Lim NR, Yeap YY, Ang CS, Williamson NA, Bogoyevitch MA, Quinn LM, Ng DC. Cell Cycle. 2016;15(3):413-24. doi: 10.1080/15384101.2015.1127472. PubMed
D-repeat in the XIST gene is required for X chromosome inactivation. Lv Q, Yuan L, Song Y, Sui T, Li Z, Lai L. RNA Biol. 2016 Jan 19:0. PubMed
Lack of MG53 in human heart precludes utility as a biomarker of myocardial injury or endogenous cardioprotective factor. Lemckert FA, Bournazos A, Eckert DM, Kenzler M, Hawkes JM, Butler TL, Ceely B, North KN, Winlaw DS, Egan JR, Cooper ST. Cardiovasc Res. 2016 Jan 19. pii: cvw017. PubMed
A Minor Subset of Super Elongation Complexes Plays a Predominant Role in Reversing HIV-1 Latency. Li Z, Lu H, Zhou Q. Mol Cell Biol. 2016 Feb 1;36(7):1194-205. doi: 10.1128/MCB.00994-15. PubMed
The Tyrosine Kinase Pyk2 Contributes to Complement-Mediated Phagocytosis in Murine Macrophages. Paone C, Rodrigues N, Ittner E, Santos C, Buntru A, Hauck CR. J Innate Immun. 2016 Feb 6. PubMed
Tandem repeat knockout utilizing the CRISPR/Cas9 system in human cells. Lv Q, Lai L, Yuan L, Song Y, Sui T, Li Z. Gene. 2016 May 15;582(2):122-7. doi: 10.1016/j.gene.2016.02.013. Epub 2016 Feb 9. PubMed
Human GBP1 does not localise to pathogen vacuoles but restricts Toxoplasma gondii. Johnston AC, Piro A, Clough B, Siew M, Virreira Winter S, Coers J, Frickel EM. Cell Microbiol. 2016 Feb 13. doi: 10.1111/cmi.12579. PubMed
Dynamics of CDKN1A in Single Cells Defined by an Endogenous Fluorescent Tagging Toolkit. Stewart-Ornstein J, Lahav G. Cell Rep. 2016 Feb 10. pii: S2211-1247(16)30023-7. doi: 10.1016/j.celrep.2016.01.045. PubMed
Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases. Yang D, Scavuzzo MA, Chmielowiec J, Sharp R, Bajic A, Borowiak M. Sci Rep. 2016 Feb 18;6:21264. doi: 10.1038/srep21264. PubMed
CHO cells knocked out for TSC2 display an improved productivity of antibodies under fed batch conditions. McVey D, Aronov M, Rizzi G, Cowan A, Scott C, Megill J, Russell R, Tirosh B. Biotechnol Bioeng. 2016 Feb 16. doi: 10.1002/bit.25951. PubMed
RNA-binding Protein PCBP2 Regulates p73 Expression and p73-dependent Antioxidant Defense. Ren C, Zhang J, Yan W, Zhang Y, Chen X. J Biol Chem. 2016 Apr 29;291(18):9629-37. doi: 10.1074/jbc.M115.712125. Epub 2016 Feb 23. PubMed
A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation. Li X, Rydzewski N, Hider A, Zhang X, Yang J, Wang W, Gao Q, Cheng X, Xu H. Nat Cell Biol. 2016 Apr;18(4):404-17. doi: 10.1038/ncb3324. Epub 2016 Mar 7. PubMed
Overcoming doxorubicin resistance of cancer cells by Cas9-mediated gene disruption. Ha JS, Byun J, Ahn DR. Sci Rep. 2016 Mar 10;6:22847. doi: 10.1038/srep22847. PubMed
AMPK Activation and Metabolic Reprogramming by Tamoxifen through Estrogen Receptor-Independent Mechanisms Suggests New Uses for This Therapeutic Modality in Cancer Treatment. Daurio NA, Tuttle SW, Worth AJ, Song EY, Davis JM, Snyder NW, Blair IA, Koumenis C. Cancer Res. 2016 Jun 1;76(11):3295-306. doi: 10.1158/0008-5472.CAN-15-2197. Epub 2016 Mar 28. PubMed
Development of Novel Small Hairpin RNAs That do not Require Processing by Dicer or AGO2. Ohno S, Itano K, Harada Y, Asada K, Oikawa K, Kashiwazako M, Okuyama H, Kumagai K, Takanashi M, Sudo K, Ikeda N, Kuroda M. Mol Ther. 2016 Aug;24(7):1278-89. doi: 10.1038/mt.2016.81. Epub 2016 Apr 25. PubMed
Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network. Crevenna AH, Blank B, Maiser A, Emin D, Prescher J, Beck G, Kienzle C, Bartnik K, Habermann B, Pakdel M, Leonhardt H, Lamb DC, von Blume J. J Cell Biol. 2016 May 9;213(3):305-14. doi: 10.1083/jcb.201601089. Epub 2016 May 2. PubMed
Efficient generation of FVII gene knockout mice using CRISPR/Cas9 nuclease and truncated guided RNAs. An L, Hu Y, Chang S, Zhu X, Ling P, Zhang F, Liu J, Liu Y, Chen Y, Yang L, Presicce GA, Du F. Sci Rep. 2016 May 3;6:25199. doi: 10.1038/srep25199. PubMed
AMPK Causes Cell Cycle Arrest in LKB1-deficient Cells via Activation of CAMKK2. Fogarty S, Ross FA, Vara Ciruelos D, Gray A, Gowans GJ, Hardie DG. Mol Cancer Res. 2016 May 2. pii: molcanres.0479.2015. PubMed
The antitumor toxin CD437 is a direct inhibitor of DNA polymerase alpha. Han T, Goralski M, Capota E, Padrick SB, Kim J, Xie Y, Nijhawan D. Nat Chem Biol. 2016 Jul;12(7):511-5. doi: 10.1038/nchembio.2082. Epub 2016 May 16. PubMed
Golgb1 regulates protein glycosylation and is crucial for mammalian palate development. Lan Y, Zhang N, Liu H, Xu J, Jiang R. Development. 2016 Jul 1;143(13):2344-55. doi: 10.1242/dev.134577. Epub 2016 May 25. PubMed
Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/Cas9-Mediated Gene Editing. Bierle CJ, Anderholm KM, Wang JB, McVoy MA, Schleiss MR. J Virol. 2016 Jul 11;90(15):6989-98. doi: 10.1128/JVI.00139-16. Print 2016 Aug 1. PubMed
Keratin 8-deletion induced colitis predisposes to murine colorectal cancer enforced by the inflammasome and IL-22 pathway. Misiorek JO, Lahdeniemi IA, Nystrom JH, Paramonov VM, Gullmets JA, Saarento H, Rivero-Muller A, Husoy T, Taimen P, Toivola DM. Carcinogenesis. 2016 May 27. pii: bgw063. PubMed
Immuno-targeting the multifunctional CD38 using nanobody. Li T, Qi S, Unger M, Hou YN, Deng QW, Liu J, Lam CM, Wang XW, Xin D, Zhang P, Koch-Nolte F, Hao Q, Zhang H, Lee HC, Zhao YJ. Sci Rep. 2016 Jun 2;6:27055. doi: 10.1038/srep27055. PubMed
A conserved phosphatase destroys toxic glycolytic side products in mammals and yeast. Collard F, Baldin F, Gerin I, Bolsee J, Noel G, Graff J, Veiga-da-Cunha M, Stroobant V, Vertommen D, Houddane A, Rider MH, Linster CL, Van Schaftingen E, Bommer GT. Nat Chem Biol. 2016 Aug;12(8):601-7. doi: 10.1038/nchembio.2104. Epub 2016 Jun 13. PubMed
Predominant Expression of Hybrid N-Glycans Has Distinct Cellular Roles Relative to Complex and Oligomannose N-Glycans. Hall MK, Weidner DA, Zhu Y, Dayal S, Whitman AA, Schwalbe RA. Int J Mol Sci. 2016 Jun 13;17(6). pii: E925. doi: 10.3390/ijms17060925. PubMed
The ubiquitin-like protein FAT10 stabilizes eEF1A1 expression to promote tumor proliferation in a complex manner. Liu X, Chen L, Ge J, Yan C, Huang Z, Hu J, Wen C, Li M, Huang D, Qiu Y, Hao H, Yuan R, Lei J, Yu X, Shao J. Cancer Res. 2016 Jun 16. pii: canres.3118.2015. PubMed
Fatostatin blocks ER exit of SCAP but inhibits cell growth in a SCAP-independent manner. Shao W, Machamer CE, Espenshade PJ. J Lipid Res. 2016 Jun 20. pii: jlr.M069583. PubMed
Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H196. Marthaler AG, Schmid B, Tubsuwan A, Poulsen UB, Engelbrecht AF, Mau-Holzmann UA, Hyttel P, Nielsen JE, Nielsen TT, Holst B. Stem Cell Res. 2016 Jan;16(1):162-5. doi: 10.1016/j.scr.2015.12.031. Epub 2016 Jan 3. PubMed
Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H266. Marthaler AG, Tubsuwan A, Schmid B, Poulsen UB, Engelbrecht AF, Mau-Holzmann UA, Hyttel P, Nielsen TT, Nielsen JE, Holst B. Stem Cell Res. 2016 Jan;16(1):202-5. doi: 10.1016/j.scr.2015.12.048. Epub 2016 Jan 15. PubMed
Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase. Baumann S, Hennet T. J Biol Chem. 2016 Aug 26;291(35):18514-24. doi: 10.1074/jbc.M116.723379. Epub 2016 Jul 11. PubMed
A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. Lambrus BG, Daggubati V, Uetake Y, Scott PM, Clutario KM, Sluder G, Holland AJ. J Cell Biol. 2016 Jul 18;214(2):143-53. doi: 10.1083/jcb.201604054. PubMed
53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. Meitinger F, Anzola JV, Kaulich M, Richardson A, Stender JD, Benner C, Glass CK, Dowdy SF, Desai A, Shiau AK, Oegema K. J Cell Biol. 2016 Jul 18;214(2):155-66. doi: 10.1083/jcb.201604081. PubMed
Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. Kubota Y, Nomura K, Katoh Y, Yamashita R, Kaneko K, Furuyama K. J Biol Chem. 2016 Sep 23;291(39):20516-29. doi: 10.1074/jbc.M116.719161. Epub 2016 Aug 5. PubMed
Loss-of-function mutations in the RNA biogenesis factor NAF1 predispose to pulmonary fibrosis-emphysema. Stanley SE, Gable DL, Wagner CL, Carlile TM, Hanumanthu VS, Podlevsky JD, Khalil SE, DeZern AE, Rojas-Duran MF, Applegate CD, Alder JK, Parry EM, Gilbert WV, Armanios M. Sci Transl Med. 2016 Aug 10;8(351):351ra107. doi: 10.1126/scitranslmed.aaf7837. PubMed
CRISPR/Cas9-Mediated Trp53 and Brca2 Knockout to Generate Improved Murine Models of Ovarian High-Grade Serous Carcinoma. Walton J, Blagih J, Ennis D, Leung E, Dowson S, Farquharson M, Tookman LA, Orange C, Athineos D, Mason S, Stevenson D, Blyth K, Strathdee D, Balkwill FR, Vousden K, Lockley M, McNeish IA. Cancer Res. 2016 Oct 15;76(20):6118-6129. Epub 2016 Aug 16. PubMed
CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice. Oji A, Noda T, Fujihara Y, Miyata H, Kim YJ, Muto M, Nozawa K, Matsumura T, Isotani A, Ikawa M. Sci Rep. 2016 Aug 17;6:31666. doi: 10.1038/srep31666. PubMed
The Non-coding Mammary Carcinoma Susceptibility Locus, Mcs5c, Regulates Pappa Expression via Age-Specific Chromatin Folding and Allele-Dependent DNA Methylation. Henning AN, Haag JD, Smits BM, Gould MN. PLoS Genet. 2016 Aug 18;12(8):e1006261. doi: 10.1371/journal.pgen.1006261. eCollection 2016 Aug. PubMed
An endosomal tether undergoes an entropic collapse to bring vesicles together. Murray DH, Jahnel M, Lauer J, Avellaneda MJ, Brouilly N, Cezanne A, Morales-Navarrete H, Perini ED, Ferguson C, Lupas AN, Kalaidzidis Y, Parton RG, Grill SW, Zerial M. Nature. 2016 Sep 1;537(7618):107-111. doi: 10.1038/nature19326. Epub 2016 Aug 24. PubMed
Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex. Zhang X, Chen MH, Wu X, Kodani A, Fan J, Doan R, Ozawa M, Ma J, Yoshida N, Reiter JF, Black DL, Kharchenko PV, Sharp PA, Walsh CA. Cell. 2016 Aug 25;166(5):1147-1162.e15. doi: 10.1016/j.cell.2016.07.025. PubMed
An Effective Molecular Target Site in Hepatitis B Virus S Gene for Cas9 Cleavage and Mutational Inactivation. Li H, Sheng C, Liu H, Liu G, Du X, Du J, Zhan L, Li P, Yang C, Qi L, Wang J, Yang X, Jia L, Xie J, Wang L, Hao R, Xu D, Tong Y, Zhou Y, Zhou J, Sun Y, Li Q, Qiu S, Song H. Int J Biol Sci. 2016 Aug 5;12(9):1104-13. doi: 10.7150/ijbs.16064. eCollection 2016. PubMed
Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Makino A, Hullin-Matsuda F, Murate M, Abe M, Tomishige N, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton I, Kobayashi T. Mol Biol Cell. 2016 Nov 1;27(21):3293-3304. Epub 2016 Aug 31. PubMed
CRISPR-Cas9 directed knock-out of a constitutively expressed gene using lance array nanoinjection. Sessions JW, Skousen CS, Price KD, Hanks BW, Hope S, Alder JK, Jensen BD. Springerplus. 2016 Sep 9;5(1):1521. doi: 10.1186/s40064-016-3037-0. eCollection 2016. PubMed
Quantitative Lipid Droplet Proteome Analysis Identifies Annexin A3 as a Cofactor for HCV Particle Production. Rosch K, Kwiatkowski M, Hofmann S, Schobel A, Gruttner C, Wurlitzer M, Schluter H, Herker E. Cell Rep. 2016 Sep 20;16(12):3219-31. doi: 10.1016/j.celrep.2016.08.052. PubMed
The Circadian Rhythm Gene Arntl2 Is a Metastasis Susceptibility Gene for Estrogen Receptor-Negative Breast Cancer. Ha NH, Long J, Cai Q, Shu XO, Hunter KW. PLoS Genet. 2016 Sep 22;12(9):e1006267. doi: 10.1371/journal.pgen.1006267. eCollection 2016 Sep. PubMed
RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes. Rose NR, King HW, Blackledge NP, Fursova NA, Ember KJ, Fischer R, Kessler BM, Klose RJ. Elife. 2016 Oct 5;5. pii: e18591. doi: 10.7554/eLife.18591. PubMed
ETAA1 acts at stalled replication forks to maintain genome integrity. Bass TE, Luzwick JW, Kavanaugh G, Carroll C, Dungrawala H, Glick GG, Feldkamp MD, Putney R, Chazin WJ, Cortez D. Nat Cell Biol. 2016 Nov;18(11):1185-1195. doi: 10.1038/ncb3415. Epub 2016 Oct 10. PubMed
The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane. Tanaka Y, Ono N, Shima T, Tanaka G, Katoh Y, Nakayama K, Takatsu H, Shin HW. Mol Biol Cell. 2016 Dec 1;27(24):3883-3893. Epub 2016 Oct 12. PubMed
Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis. Sato K, Kimura M, Sugiyama K, Nishikawa M, Okano Y, Nagaoka H, Nagase T, Kitade Y, Ueda H. J Biol Chem. 2016 Nov 25;291(48):25227-25238. Epub 2016 Oct 20. PubMed
Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions. Martins R, Maier J, Gorki AD, Huber KV, Sharif O, Starkl P, Saluzzo S, Quattrone F, Gawish R, Lakovits K, Aichinger MC, Radic-Sarikas B, Lardeau CH, Hladik A, Korosec A, Brown M, Vaahtomeri K, Duggan M, Kerjaschki D, Esterbauer H, Colinge J, Eisenbarth SC, Decker T, Bennett KL, Kubicek S, Sixt M, Superti-Furga G, Knapp S. Nat Immunol. 2016 Dec;17(12):1361-1372. doi: 10.1038/ni.3590. Epub 2016 Oct 31. PubMed
RHO binding to FAM65A regulates Golgi reorientation during cell migration. Mardakheh FK, Self A, Marshall CJ. J Cell Sci. 2016 Dec 15;129(24):4466-4479. Epub 2016 Nov 2. PubMed
Inactivation of the type I interferon pathway reveals long double-stranded RNA-mediated RNA interference in mammalian cells. Maillard PV, Van der Veen AG, Deddouche-Grass S, Rogers NC, Merits A, Reis E Sousa C. EMBO J. 2016 Dec 1;35(23):2505-2518. Epub 2016 Nov 4. PubMed
CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening. Koferle A, Worf K, Breunig C, Baumann V, Herrero J, Wiesbeck M, Hutter LH, Gotz M, Fuchs C, Beck S, Stricker SH. BMC Genomics. 2016 Nov 14;17(1):917. PubMed
Generation of a gene-corrected isogenic control cell line from an Alzheimer's disease patient iPSC line carrying a A79V mutation in PSEN1. Pires C, Schmid B, Petraeus C, Poon A, Nimsanor N, Nielsen TT, Waldemar G, Hjermind LE, Nielsen JE, Hyttel P, Freude KK. Stem Cell Res. 2016 Sep;17(2):285-288. doi: 10.1016/j.scr.2016.08.002. Epub 2016 Aug 7. PubMed
Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9. Herring A, Messana A, Bara AM, Hazelbaker DZ, Eggan K, Barrett LE. Stem Cell Res. 2016 Sep;17(2):430-432. doi: 10.1016/j.scr.2016.09.009. Epub 2016 Sep 16. PubMed
Ubiquitylation of Ku80 by RNF126 promotes completion of NHEJ-mediated DNA repair. Ishida N, Nakagawa T, Iemura SI, Yasui A, Shima H, Katoh Y, Nagasawa Y, Natsume T, Igarashi K, Nakayama K. Mol Cell Biol. 2016 Nov 28. pii: MCB.00347-16. PubMed
The Hippo Pathway Kinases LATS1/2 Suppress Cancer Immunity. Moroishi T, Hayashi T, Pan WW, Fujita Y, Holt MV, Qin J, Carson DA, Guan KL. Cell. 2016 Dec 1;167(6):1525-1539.e17. doi: 10.1016/j.cell.2016.11.005. PubMed
Characterization of Hippo Pathway Components by Gene Inactivation. Plouffe SW, Meng Z, Lin KC, Lin B, Hong AW, Chun JV, Guan KL. Mol Cell. 2016 Dec 1;64(5):993-1008. doi: 10.1016/j.molcel.2016.10.034. PubMed
Regulation of ciliary retrograde protein trafficking by Joubert syndrome proteins ARL13B and INPP5E. Nozaki S, Katoh Y, Terada M, Michisaka S, Funabashi T, Takahashi S, Kontani K, Nakayama K. J Cell Sci. 2016 Dec 7. pii: jcs.197004. PubMed
Intraflagellar transport-A complex mediates ciliary entry as well as retrograde trafficking of ciliary G protein-coupled receptors. Hirano T, Katoh Y, Nakayama K. Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-11-0813. PubMed
CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma. Phelps MP, Bailey JN, Vleeshouwer-Neumann T, Chen EY. Proc Natl Acad Sci U S A. 2016 Dec 27;113(52):15090-15095. doi: 10.1073/pnas.1610270114. Epub 2016 Dec 12. PubMed
Delta40p53 suppresses tumor cell proliferation and induces cellular senescence in hepatocellular carcinoma cells. Ota A, Nakao H, Sawada Y, Karnan S, Wahiduzzaman M, Inoue T, Kobayashi Y, Yamamoto T, Ishii N, Ohashi T, Nakade Y, Sato K, Itoh K, Konishi H, Hosokawa Y, Yoneda M. J Cell Sci. 2016 Dec 15. pii: jcs.190736. PubMed
Random Splicing of Several Exons Caused by a Single Base Change in the Target Exon of CRISPR/Cas9 Mediated Gene Knockout. Kapahnke M, Banning A, Tikkanen R. Cells. 2016 Dec 14;5(4). pii: E45. PubMed
Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Makino S, Fukumura R, Gondo Y. Sci Rep. 2016 Dec 21;6:39608. doi: 10.1038/srep39608. PubMed
Deletion of the GAA repeats from the human frataxin gene using the CRISPR-Cas9 system in YG8R-derived cells and mouse models of Friedreich ataxia. Ouellet DL, Cherif K, Rousseau J, Tremblay JP. Gene Ther. 2017 May;24(5):265-274. doi: 10.1038/gt.2016.89. Epub 2016 Dec 26. PubMed
TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP). Li MM, Lau Z, Cheung P, Aguilar EG, Schneider WM, Bozzacco L, Molina H, Buehler E, Takaoka A, Rice CM, Felsenfeld DP, MacDonald MR. PLoS Pathog. 2017 Jan 6;13(1):e1006145. doi: 10.1371/journal.ppat.1006145. PubMed
A Fluorophore Fusion-construct of Human Profilin I with Non-compromized poly(L-Proline) Binding capacity suitable for Imaging. Nejedla M, Li Z, Masser AE, Biancospino M, Spiess M, Mackowiak SD, Friedlander MR, Karlsson R. J Mol Biol. 2017 Jan 7. pii: S0022-2836(17)30018-9. doi: 10.1016/j.jmb.2017.01.004. PubMed
Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Funabashi T, Katoh Y, Michisaka S, Terada M, Sugawa M, Nakayama K. Mol Biol Cell. 2017 Mar 1;28(5):624-633. doi: 10.1091/mbc.E16-09-0648. Epub 2017 Jan 11. PubMed
Structural basis for ELL2 and AFF4 activation of HIV-1 proviral transcription. Qi S, Li Z, Schulze-Gahmen U, Stjepanovic G, Zhou Q, Hurley JH. Nat Commun. 2017 Jan 30;8:14076. doi: 10.1038/ncomms14076. PubMed
Filopodia formation and endosome clustering induced by mutant plus-end-directed myosin VI. Masters TA, Buss F. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1595-1600. doi: 10.1073/pnas.1616941114. Epub 2017 Jan 31. PubMed
Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system. Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, Nakayama K. Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051. PubMed

Associated Plasmids

MARK4 inhibits Hippo signaling to promote proliferation and migration of breast cancer cells. Heidary Arash E, Shiban A, Song S, Attisano L. EMBO Rep. 2017 Mar;18(3):420-436. doi: 10.15252/embr.201642455. Epub 2017 Feb 9. PubMed
ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Leung JW, Makharashvili N, Agarwal P, Chiu LY, Pourpre R, Cammarata MB, Cannon JR, Sherker A, Durocher D, Brodbelt JS, Paull TT, Miller KM. Genes Dev. 2017 Feb 1;31(3):260-274. doi: 10.1101/gad.292516.116. Epub 2017 Feb 27. PubMed
MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells. Ito K, Eguchi Y, Imagawa Y, Akai S, Mochizuki H, Tsujimoto Y. Cell Death Discov. 2017 Feb 27;3:17013. doi: 10.1038/cddiscovery.2017.13. eCollection 2017. PubMed
Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells. Lin L, Petersen TS, Jensen KT, Bolund L, Kuhn R, Luo Y. J Biotechnol. 2017 Mar 1;247:42-49. doi: 10.1016/j.jbiotec.2017.02.024. PubMed

Associated Plasmids

CRISPR system for genome engineering: the application for autophagy study. Cui J, Chew SJL, Shi Y, Gong Z, Shen HM. BMB Rep. 2017 May;50(5):247-256. PubMed
Re-evaluating the roles of myosin 18Aalpha and F-actin in determining Golgi morphology. Bruun K, Beach JR, Heissler SM, Remmert K, Sellers JR, Hammer JA. Cytoskeleton (Hoboken). 2017 May;74(5):205-218. doi: 10.1002/cm.21364. Epub 2017 May 10. PubMed
Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus. De Jaime-Soguero A, Aulicino F, Ertaylan G, Griego A, Cerrato A, Tallam A, Del Sol A, Cosma MP, Lluis F. PLoS Genet. 2017 Mar 27;13(3):e1006682. doi: 10.1371/journal.pgen.1006682. eCollection 2017 Mar. PubMed
MED12-related XLID disorders are dose-dependent of immediate early genes (IEGs) expression. Donnio LM, Bidon B, Hashimoto S, May M, Epanchintsev A, Ryan C, Allen W, Hackett A, Gecz J, Skinner C, Stevenson RE, de Brouwer APM, Coutton C, Francannet C, Jouk PS, Schwartz CE, Egly JM. Hum Mol Genet. 2017 Jun 1;26(11):2062-2075. doi: 10.1093/hmg/ddx099. PubMed
A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3'end using CRISPR-Cas9 genome editing technique. Basu S, Adams L, Guhathakurta S, Kim YS. Sci Rep. 2017 Apr 4;8:45883. doi: 10.1038/srep45883. PubMed
FKBP8 recruits LC3A to mediate Parkin-independent mitophagy. Bhujabal Z, Birgisdottir AB, Sjottem E, Brenne HB, Overvatn A, Habisov S, Kirkin V, Lamark T, Johansen T. EMBO Rep. 2017 Jun;18(6):947-961. doi: 10.15252/embr.201643147. Epub 2017 Apr 5. PubMed
Removal of Integrated Hepatitis B Virus DNA Using CRISPR-Cas9. Li H, Sheng C, Wang S, Yang L, Liang Y, Huang Y, Liu H, Li P, Yang C, Yang X, Jia L, Xie J, Wang L, Hao R, Du X, Xu D, Zhou J, Li M, Sun Y, Tong Y, Li Q, Qiu S, Song H. Front Cell Infect Microbiol. 2017 Mar 22;7:91. doi: 10.3389/fcimb.2017.00091. eCollection 2017. PubMed
MIEF1/2 function as adaptors to recruit Drp1 to mitochondria and regulate the association of Drp1 with Mff. Yu R, Liu T, Jin SB, Ning C, Lendahl U, Nister M, Zhao J. Sci Rep. 2017 Apr 13;7(1):880. doi: 10.1038/s41598-017-00853-x. PubMed
RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly. Nishijima Y, Hagiya Y, Kubo T, Takei R, Katoh Y, Nakayama K. Mol Biol Cell. 2017 Jun 15;28(12):1652-1666. doi: 10.1091/mbc.E17-01-0017. Epub 2017 Apr 20. PubMed
A Gene Regulatory Network Balances Neural and Mesoderm Specification during Vertebrate Trunk Development. Gouti M, Delile J, Stamataki D, Wymeersch FJ, Huang Y, Kleinjung J, Wilson V, Briscoe J. Dev Cell. 2017 May 8;41(3):243-261.e7. doi: 10.1016/j.devcel.2017.04.002. Epub 2017 Apr 27. PubMed
Glucocorticoid Receptor:MegaTrans Switching Mediates the Repression of an ERalpha-Regulated Transcriptional Program. Yang F, Ma Q, Liu Z, Li W, Tan Y, Jin C, Ma W, Hu Y, Shen J, Ohgi KA, Telese F, Liu W, Rosenfeld MG. Mol Cell. 2017 May 4;66(3):321-331.e6. doi: 10.1016/j.molcel.2017.03.019. PubMed
Golga5 is dispensable for mouse embryonic development and postnatal survival. McGee LJ, Jiang AL, Lan Y. Genesis. 2017 Jul;55(7). doi: 10.1002/dvg.23039. Epub 2017 Jun 2. PubMed
The New Chemical Reporter 6-Alkynyl-6-deoxy-GlcNAc Reveals O-GlcNAc Modification of the Apoptotic Caspases That Can Block the Cleavage/Activation of Caspase-8. Chuh KN, Batt AR, Zaro BW, Darabedian N, Marotta NP, Brennan CK, Amirhekmat A, Pratt MR. J Am Chem Soc. 2017 Jun 14;139(23):7872-7885. doi: 10.1021/jacs.7b02213. Epub 2017 May 31. PubMed
Activation of Adiponectin Receptor Regulates Proprotein Convertase Subtilisin/Kexin Type 9 Expression and Inhibits Lesions in ApoE-Deficient Mice. Sun L, Yang X, Li Q, Zeng P, Liu Y, Liu L, Chen Y, Yu M, Ma C, Li X, Li Y, Zhang R, Zhu Y, Miao QR, Han J, Duan Y. Arterioscler Thromb Vasc Biol. 2017 Jul;37(7):1290-1300. doi: 10.1161/ATVBAHA.117.309630. Epub 2017 May 25. PubMed
Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency. Jensen KT, Floe L, Petersen TS, Huang J, Xu F, Bolund L, Luo Y, Lin L. FEBS Lett. 2017 Jul;591(13):1892-1901. doi: 10.1002/1873-3468.12707. Epub 2017 Jun 28. PubMed
Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2. Tchasovnikarova IA, Timms RT, Douse CH, Roberts RC, Dougan G, Kingston RE, Modis Y, Lehner PJ. Nat Genet. 2017 Jul;49(7):1035-1044. doi: 10.1038/ng.3878. Epub 2017 Jun 5. PubMed
Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time. White CW, Vanyai HK, See HB, Johnstone EKM, Pfleger KDG. Sci Rep. 2017 Jun 9;7(1):3187. doi: 10.1038/s41598-017-03486-2. PubMed
Viral Replication Complexes Are Targeted by LC3-Guided Interferon-Inducible GTPases. Biering SB, Choi J, Halstrom RA, Brown HM, Beatty WL, Lee S, McCune BT, Dominici E, Williams LE, Orchard RC, Wilen CB, Yamamoto M, Coers J, Taylor GA, Hwang S. Cell Host Microbe. 2017 Jul 12;22(1):74-85.e7. doi: 10.1016/j.chom.2017.06.005. Epub 2017 Jun 29. PubMed
Syk Plays a Critical Role in the Expression and Activation of IRAK1 in LPS-Treated Macrophages. Park JG, Son YJ, Yoo BC, Yang WS, Kim JH, Kim JH, Cho JY. Mediators Inflamm. 2017;2017:1506248. doi: 10.1155/2017/1506248. Epub 2017 Jun 7. PubMed
Regulatory Expansion in Mammals of Multivalent hnRNP Assemblies that Globally Control Alternative Splicing. Gueroussov S, Weatheritt RJ, O'Hanlon D, Lin ZY, Narula A, Gingras AC, Blencowe BJ. Cell. 2017 Jul 13;170(2):324-339.e23. doi: 10.1016/j.cell.2017.06.037. PubMed
Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer. Nabet BY, Qiu Y, Shabason JE, Wu TJ, Yoon T, Kim BC, Benci JL, DeMichele AM, Tchou J, Marcotrigiano J, Minn AJ. Cell. 2017 Jul 13;170(2):352-366.e13. doi: 10.1016/j.cell.2017.06.031. PubMed
Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK. Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, Lin SC. Nature. 2017 Aug 3;548(7665):112-116. doi: 10.1038/nature23275. Epub 2017 Jul 19. PubMed
A time-resolved molecular map of the macrophage response to VSV infection. Kandasamy RK, Vladimer GI, Snijder B, Muller AC, Rebsamen M, Bigenzahn JW, Moskovskich A, Sabler M, Stefanovic A, Scorzoni S, Bruckner M, Penz T, Cleary C, Kralovics R, Colinge J, Bennett KL, Superti-Furga G. NPJ Syst Biol Appl. 2016 Oct 27;2:16027. doi: 10.1038/npjsba.2016.27. eCollection 2016. PubMed
Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families. Voloshanenko O, Gmach P, Winter J, Kranz D, Boutros M. FASEB J. 2017 Nov;31(11):4832-4844. doi: 10.1096/fj.201700144R. Epub 2017 Jul 21. PubMed
The Axis of CXCR4/SDF-1 Plays a Role in Colon Cancer Cell Adhesion Through Regulation of the AKT and IGF1R Signalling Pathways. Zheng F, Zhang Z, Flamini V, Jiang WG, Cui Y. Anticancer Res. 2017 Aug;37(8):4361-4369. doi: 10.21873/anticanres.11830. PubMed
Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration. Banks CJ, Rodriguez NW, Gashler KR, Pandya RR, Mortenson JB, Whited MD, Soderblom EJ, Thompson JW, Moseley MA, Reddi AR, Tessem JS, Torres MP, Bikman BT, Andersen JL. Mol Cell Biol. 2017 Sep 26;37(20). pii: MCB.00354-17. doi: 10.1128/MCB.00354-17. Print 2017 Oct 15. PubMed
Regulation of Hippo pathway transcription factor TEAD by p38 MAPK-induced cytoplasmic translocation. Lin KC, Moroishi T, Meng Z, Jeong HS, Plouffe SW, Sekido Y, Han J, Park HW, Guan KL. Nat Cell Biol. 2017 Jul 28;19(8):996-1002. doi: 10.1038/ncb3581. PubMed
Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein. Scott CA, Marsden AN, Rebagliati MR, Zhang Q, Chamling X, Searby CC, Baye LM, Sheffield VC, Slusarski DC. PLoS Genet. 2017 Jul 28;13(7):e1006936. doi: 10.1371/journal.pgen.1006936. eCollection 2017 Jul. PubMed
IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells. Fu Y, Zhou Z, Wang H, Gong P, Guo R, Wang J, Lu X, Qi F, Liu L. FEBS Open Bio. 2017 Jun 29;7(8):1102-1110. doi: 10.1002/2211-5463.12246. eCollection 2017 Aug. PubMed
Identification and characterization of a long non-coding RNA up-regulated during HIV-1 infection. Postler TS, Pantry SN, Desrosiers RC, Ghosh S. Virology. 2017 Nov;511:30-39. doi: 10.1016/j.virol.2017.08.006. Epub 2017 Aug 10. PubMed
Open Chromatin Profiling in hiPSC-Derived Neurons Prioritizes Functional Noncoding Psychiatric Risk Variants and Highlights Neurodevelopmental Loci. Forrest MP, Zhang H, Moy W, McGowan H, Leites C, Dionisio LE, Xu Z, Shi J, Sanders AR, Greenleaf WJ, Cowan CA, Pang ZP, Gejman PV, Penzes P, Duan J. Cell Stem Cell. 2017 Sep 7;21(3):305-318.e8. doi: 10.1016/j.stem.2017.07.008. Epub 2017 Aug 10. PubMed
Sensitized mutagenesis screen in Factor V Leiden mice identifies thrombosis suppressor loci. Westrick RJ, Tomberg K, Siebert AE, Zhu G, Winn ME, Dobies SL, Manning SL, Brake MA, Cleuren AC, Hobbs LM, Mishack LM, Johnston AJ, Kotnik E, Siemieniak DR, Xu J, Li JZ, Saunders TL, Ginsburg D. Proc Natl Acad Sci U S A. 2017 Sep 5;114(36):9659-9664. doi: 10.1073/pnas.1705762114. Epub 2017 Aug 21. PubMed
SIRT7 antagonizes TGF-beta signaling and inhibits breast cancer metastasis. Tang X, Shi L, Xie N, Liu Z, Qian M, Meng F, Xu Q, Zhou M, Cao X, Zhu WG, Liu B. Nat Commun. 2017 Aug 22;8(1):318. doi: 10.1038/s41467-017-00396-9. PubMed
Lack of Trex1 Causes Systemic Autoimmunity despite the Presence of Antiretroviral Drugs. Achleitner M, Kleefisch M, Hennig A, Peschke K, Polikarpova A, Oertel R, Gabriel B, Schulze L, Lindeman D, Gerbaulet A, Fiebig U, Lee-Kirsch MA, Roers A, Behrendt R. J Immunol. 2017 Oct 1;199(7):2261-2269. doi: 10.4049/jimmunol.1700714. Epub 2017 Aug 23. PubMed
Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes. Ishizu T, Higo S, Masumura Y, Kohama Y, Shiba M, Higo T, Shibamoto M, Nakagawa A, Morimoto S, Takashima S, Hikoso S, Sakata Y. Sci Rep. 2017 Aug 24;7(1):9363. doi: 10.1038/s41598-017-09716-x. PubMed
Postnatal Cardiac Gene Editing Using CRISPR/Cas9 With AAV9-Mediated Delivery of Short Guide RNAs Results in Mosaic Gene Disruption. Johansen AK, Molenaar B, Versteeg D, Leitoguinho AR, Demkes C, Spanjaard B, de Ruiter H, Akbari Moqadam F, Kooijman L, Zentilin L, Giacca M, van Rooij E. Circ Res. 2017 Oct 27;121(10):1168-1181. doi: 10.1161/CIRCRESAHA.116.310370. Epub 2017 Aug 29. PubMed

Associated Plasmids

FMNL2 and -3 regulate Golgi architecture and anterograde transport downstream of Cdc42. Kage F, Steffen A, Ellinger A, Ranftler C, Gehre C, Brakebusch C, Pavelka M, Stradal T, Rottner K. Sci Rep. 2017 Aug 29;7(1):9791. doi: 10.1038/s41598-017-09952-1. PubMed
Proteolytic processing of lysyl oxidase-like-2 in the extracellular matrix is required for crosslinking of basement membrane collagen IV. Lopez-Jimenez AJ, Basak T, Vanacore RM. J Biol Chem. 2017 Oct 13;292(41):16970-16982. doi: 10.1074/jbc.M117.798603. Epub 2017 Sep 1. PubMed
Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity. Vujanovic M, Krietsch J, Raso MC, Terraneo N, Zellweger R, Schmid JA, Taglialatela A, Huang JW, Holland CL, Zwicky K, Herrador R, Jacobs H, Cortez D, Ciccia A, Penengo L, Lopes M. Mol Cell. 2017 Sep 7;67(5):882-890.e5. doi: 10.1016/j.molcel.2017.08.010. PubMed
CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression. Ren G, Jin W, Cui K, Rodrigez J, Hu G, Zhang Z, Larson DR, Zhao K. Mol Cell. 2017 Sep 21;67(6):1049-1058.e6. doi: 10.1016/j.molcel.2017.08.026. PubMed
Effects of genomic disruption of a guanine quadruplex in the 5' UTR of the Bcl-2 mRNA in melanoma cells. Serikawa T, Eberle J, Kurreck J. FEBS Lett. 2017 Nov;591(21):3649-3659. doi: 10.1002/1873-3468.12855. Epub 2017 Oct 3. PubMed
EHD Proteins Cooperate to Generate Caveolar Clusters and to Maintain Caveolae during Repeated Mechanical Stress. Yeow I, Howard G, Chadwick J, Mendoza-Topaz C, Hansen CG, Nichols BJ, Shvets E. Curr Biol. 2017 Oct 9;27(19):2951-2962.e5. doi: 10.1016/j.cub.2017.07.047. Epub 2017 Sep 21. PubMed
Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers. Yau EH, Kummetha IR, Lichinchi G, Tang R, Zhang Y, Rana TM. Cancer Res. 2017 Nov 15;77(22):6330-6339. doi: 10.1158/0008-5472.CAN-17-2043. Epub 2017 Sep 27. PubMed
RhoC regulates the actin remodeling required for phagosome formation during FcgammaR-mediated phagocytosis. Egami Y, Kawai K, Araki N. J Cell Sci. 2017 Dec 15;130(24):4168-4179. doi: 10.1242/jcs.202739. Epub 2017 Nov 7. PubMed
Galphai is required for carvedilol-induced beta1 adrenergic receptor beta-arrestin biased signaling. Wang J, Hanada K, Staus DP, Makara MA, Dahal GR, Chen Q, Ahles A, Engelhardt S, Rockman HA. Nat Commun. 2017 Nov 22;8(1):1706. doi: 10.1038/s41467-017-01855-z. PubMed
Comparative genomics reveals that loss of lunatic fringe (LFNG) promotes melanoma metastasis. Del Castillo Velasco-Herrera M, van der Weyden L, Nsengimana J, Speak AO, Sjoberg MK, Bishop DT, Jonsson G, Newton-Bishop J, Adams DJ. Mol Oncol. 2018 Feb;12(2):239-255. doi: 10.1002/1878-0261.12161. Epub 2018 Jan 7. PubMed
ATM and CDK2 control chromatin remodeler CSB to inhibit RIF1 in DSB repair pathway choice. Batenburg NL, Walker JR, Noordermeer SM, Moatti N, Durocher D, Zhu XD. Nat Commun. 2017 Dec 4;8(1):1921. doi: 10.1038/s41467-017-02114-x. PubMed
Therapeutic relevance of the PP2A-B55 inhibitory kinase MASTL/Greatwall in breast cancer. Alvarez-Fernandez M, Sanz-Flores M, Sanz-Castillo B, Salazar-Roa M, Partida D, Zapatero-Solana E, Ali HR, Manchado E, Lowe S, VanArsdale T, Shields D, Caldas C, Quintela-Fandino M, Malumbres M. Cell Death Differ. 2017 Dec 11. pii: 10.1038/s41418-017-0024-0. doi: 10.1038/s41418-017-0024-0. PubMed
Regulation of the cohesin-loading factor NIPBL: Role of the lncRNA NIPBL-AS1 and identification of a distal enhancer element. Zuin J, Casa V, Pozojevic J, Kolovos P, van den Hout MCGN, van Ijcken WFJ, Parenti I, Braunholz D, Baron Y, Watrin E, Kaiser FJ, Wendt KS. PLoS Genet. 2017 Dec 20;13(12):e1007137. doi: 10.1371/journal.pgen.1007137. eCollection 2017 Dec. PubMed
A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes. Bersuker K, Peterson CWH, To M, Sahl SJ, Savikhin V, Grossman EA, Nomura DK, Olzmann JA. Dev Cell. 2018 Jan 8;44(1):97-112.e7. doi: 10.1016/j.devcel.2017.11.020. Epub 2017 Dec 21. PubMed

Associated Plasmids

CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog. Pusapati GV, Kong JH, Patel BB, Krishnan A, Sagner A, Kinnebrew M, Briscoe J, Aravind L, Rohatgi R. Dev Cell. 2018 Jan 8;44(1):113-129.e8. doi: 10.1016/j.devcel.2017.12.003. Epub 2017 Dec 28. PubMed
ER-stress mobilization of death-associated protein kinase-1-dependent xenophagy counteracts mitochondria stress-induced epithelial barrier dysfunction. Lopes F, Keita AV, Saxena A, Reyes JL, Mancini NL, Al Rajabi A, Wang A, Baggio CH, Dicay M, van Dalen R, Ahn Y, Carneiro MBH, Peters NC, Rho JM, MacNaughton WK, Girardin SE, Jijon H, Philpott DJ, Soderholm JD, McKay DM. J Biol Chem. 2018 Mar 2;293(9):3073-3087. doi: 10.1074/jbc.RA117.000809. Epub 2018 Jan 9. PubMed
Cerebral organoids derived from Sandhoff disease-induced pluripotent stem cells exhibit impaired neurodifferentiation. Allende ML, Cook EK, Larman BC, Nugent A, Brady JM, Golebiowski D, Sena-Esteves M, Tifft CJ, Proia RL. J Lipid Res. 2018 Mar;59(3):550-563. doi: 10.1194/jlr.M081323. Epub 2018 Jan 22. PubMed
ERAD-dependent control of the Wnt secretory factor Evi. Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, Christianson JC, Boutros M. EMBO J. 2018 Feb 15;37(4). pii: embj.201797311. doi: 10.15252/embj.201797311. Epub 2018 Jan 29. PubMed
MGA, L3MBTL2 and E2F6 determine genomic binding of the non-canonical Polycomb repressive complex PRC1.6. Stielow B, Finkernagel F, Stiewe T, Nist A, Suske G. PLoS Genet. 2018 Jan 30;14(1):e1007193. doi: 10.1371/journal.pgen.1007193. PubMed

Associated Plasmids

PARP-1-dependent recruitment of cold-inducible RNA-binding protein promotes double-strand break repair and genome stability. Chen JK, Lin WL, Chen Z, Liu HW. Proc Natl Acad Sci U S A. 2018 Feb 20;115(8):E1759-E1768. doi: 10.1073/pnas.1713912115. Epub 2018 Feb 5. PubMed
ZBTB2 reads unmethylated CpG island promoters and regulates embryonic stem cell differentiation. Karemaker ID, Vermeulen M. EMBO Rep. 2018 Apr;19(4). pii: embr.201744993. doi: 10.15252/embr.201744993. Epub 2018 Feb 1. PubMed
DeSUMOylation switches Kaiso from activator to repressor upon hyperosmotic stress. Zhenilo S, Deyev I, Litvinova E, Zhigalova N, Kaplun D, Sokolov A, Mazur A, Prokhortchouk E. Cell Death Differ. 2018 Nov;25(11):1938-1951. doi: 10.1038/s41418-018-0078-7. Epub 2018 Feb 22. PubMed
Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1. Palstra RJ, de Crignis E, Roling MD, van Staveren T, Kan TW, van Ijcken W, Mueller YM, Katsikis PD, Mahmoudi T. Sci Adv. 2018 Feb 21;4(2):e1701729. doi: 10.1126/sciadv.1701729. eCollection 2018 Feb. PubMed
Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic alpha-helix. Burgess SG, Mukherjee M, Sabir S, Joseph N, Gutierrez-Caballero C, Richards MW, Huguenin-Dezot N, Chin JW, Kennedy EJ, Pfuhl M, Royle SJ, Gergely F, Bayliss R. EMBO J. 2018 Apr 13;37(8). pii: embj.201797902. doi: 10.15252/embj.201797902. Epub 2018 Mar 6. PubMed
A VCP inhibitor substrate trapping approach (VISTA) enables proteomic profiling of endogenous ERAD substrates. Huang EY, To M, Tran E, Dionisio LTA, Cho HJ, Baney KLM, Pataki CI, Olzmann JA. Mol Biol Cell. 2018 May 1;29(9):1021-1030. doi: 10.1091/mbc.E17-08-0514. Epub 2018 Mar 22. PubMed
Negative regulation of amino acid signaling by MAPK-regulated 4F2hc/Girdin complex. Weng L, Han YP, Enomoto A, Kitaura Y, Nagamori S, Kanai Y, Asai N, An J, Takagishi M, Asai M, Mii S, Masuko T, Shimomura Y, Takahashi M. PLoS Biol. 2018 Mar 14;16(3):e2005090. doi: 10.1371/journal.pbio.2005090. eCollection 2018 Mar. PubMed
ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability. Haahr P, Borgermann N, Guo X, Typas D, Achuthankutty D, Hoffmann S, Shearer R, Sixma TK, Mailand N. Mol Cell. 2018 Apr 5;70(1):165-174.e6. doi: 10.1016/j.molcel.2018.02.024. Epub 2018 Mar 22. PubMed
Phospholipid-flipping activity of P4-ATPase drives membrane curvature. Takada N, Naito T, Inoue T, Nakayama K, Takatsu H, Shin HW. EMBO J. 2018 May 2;37(9). pii: embj.201797705. doi: 10.15252/embj.201797705. Epub 2018 Mar 29. PubMed
Phosphorylation of Beta-3 adrenergic receptor at serine 247 by ERK MAP kinase drives lipolysis in obese adipocytes. Hong S, Song W, Zushin PH, Liu B, Jedrychowski MP, Mina AI, Deng Z, Cabarkapa D, Hall JA, Palmer CJ, Aliakbarian H, Szpyt J, Gygi SP, Tavakkoli A, Lynch L, Perrimon N, Banks AS. Mol Metab. 2018 Jun;12:25-38. doi: 10.1016/j.molmet.2018.03.012. Epub 2018 Mar 29. PubMed
Pluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity. Finley LWS, Vardhana SA, Carey BW, Alonso-Curbelo D, Koche R, Chen Y, Wen D, King B, Radler MR, Rafii S, Lowe SW, Allis CD, Thompson CB. Nat Cell Biol. 2018 May;20(5):565-574. doi: 10.1038/s41556-018-0086-3. Epub 2018 Apr 16. PubMed
MEKK2 and MEKK3 suppress Hedgehog pathway-dependent medulloblastoma by inhibiting GLI1 function. Lu J, Liu L, Zheng M, Li X, Wu A, Wu Q, Liao C, Zou J, Song H. Oncogene. 2018 Jul;37(28):3864-3878. doi: 10.1038/s41388-018-0249-5. Epub 2018 Apr 17. PubMed
Pluripotency factors functionally premark cell-type-restricted enhancers in ES cells. Kim HS, Tan Y, Ma W, Merkurjev D, Destici E, Ma Q, Suter T, Ohgi K, Friedman M, Skowronska-Krawczyk D, Rosenfeld MG. Nature. 2018 Apr;556(7702):510-514. doi: 10.1038/s41586-018-0048-8. Epub 2018 Apr 18. PubMed
CRISPR/Cas9 Mediated GFP Knock-in at the MAP1LC3B Locus in 293FT Cells Is Better for Bona Fide Monitoring Cellular Autophagy. Wu Z, Zhao J, Qiu M, Mi Z, Meng M, Guo Y, Wang H, Yuan Z. Biotechnol J. 2018 Nov;13(11):e1700674. doi: 10.1002/biot.201700674. Epub 2018 May 27. PubMed
A PAX5-OCT4-PRDM1 developmental switch specifies human primordial germ cells. Fang F, Angulo B, Xia N, Sukhwani M, Wang Z, Carey CC, Mazurie A, Cui J, Wilkinson R, Wiedenheft B, Irie N, Surani MA, Orwig KE, Reijo Pera RA. Nat Cell Biol. 2018 Jun;20(6):655-665. doi: 10.1038/s41556-018-0094-3. Epub 2018 Apr 30. PubMed
Long noncoding RNA Hoxb3os is dysregulated in autosomal dominant polycystic kidney disease and regulates mTOR signaling. Aboudehen K, Farahani S, Kanchwala M, Chan SC, Avdulov S, Mickelson A, Lee D, Gearhart MD, Patel V, Xing C, Igarashi P. J Biol Chem. 2018 Jun 15;293(24):9388-9398. doi: 10.1074/jbc.RA118.001723. Epub 2018 May 1. PubMed
Boosting ATM activity alleviates aging and extends lifespan in a mouse model of progeria. Qian M, Liu Z, Peng L, Tang X, Meng F, Ao Y, Zhou M, Wang M, Cao X, Qin B, Wang Z, Zhou Z, Wang G, Gao Z, Xu J, Liu B. Elife. 2018 May 2;7. pii: 34836. doi: 10.7554/eLife.34836. PubMed
Depletion of Nsd2-mediated histone H3K36 methylation impairs adipose tissue development and function. Zhuang L, Jang Y, Park YK, Lee JE, Jain S, Froimchuk E, Broun A, Liu C, Gavrilova O, Ge K. Nat Commun. 2018 May 4;9(1):1796. doi: 10.1038/s41467-018-04127-6. PubMed
Complement C3 Drives Autophagy-Dependent Restriction of Cyto-invasive Bacteria. Sorbara MT, Foerster EG, Tsalikis J, Abdel-Nour M, Mangiapane J, Sirluck-Schroeder I, Tattoli I, van Dalen R, Isenman DE, Rohde JR, Girardin SE, Philpott DJ. Cell Host Microbe. 2018 May 9;23(5):644-652.e5. doi: 10.1016/j.chom.2018.04.008. PubMed
The Metabolic Chemical Reporter 6-Azido-6-deoxy-glucose Further Reveals the Substrate Promiscuity of O-GlcNAc Transferase and Catalyzes the Discovery of Intracellular Protein Modification by O-Glucose. Darabedian N, Gao J, Chuh KN, Woo CM, Pratt MR. J Am Chem Soc. 2018 Jun 13;140(23):7092-7100. doi: 10.1021/jacs.7b13488. Epub 2018 May 30. PubMed
Functional analysis of juxta- and intra-membrane domains of murine APP by genome editing in Neuro2a cells. Kaneshiro N, Imaoka R, Komai M, Kashiyama T, Sakurai T, Uehara T, Takasugi N. Biochem Biophys Res Commun. 2018 Jul 2;501(4):1023-1028. doi: 10.1016/j.bbrc.2018.05.102. Epub 2018 May 23. PubMed
The Hippo pathway effector proteins YAP and TAZ have both distinct and overlapping functions in the cell. Plouffe SW, Lin KC, Moore JL 3rd, Tan FE, Ma S, Ye Z, Qiu Y, Ren B, Guan KL. J Biol Chem. 2018 Jul 13;293(28):11230-11240. doi: 10.1074/jbc.RA118.002715. Epub 2018 May 25. PubMed
FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment. Dimitrova E, Kondo T, Feldmann A, Nakayama M, Koseki Y, Konietzny R, Kessler BM, Koseki H, Klose RJ. Elife. 2018 May 29;7. pii: 37084. doi: 10.7554/eLife.37084. PubMed
Dual phosphorylation of Ric-8A enhances its ability to mediate G protein alpha subunit folding and to stimulate guanine nucleotide exchange. Papasergi-Scott MM, Stoveken HM, MacConnachie L, Chan PY, Gabay M, Wong D, Freeman RS, Beg AA, Tall GG. Sci Signal. 2018 May 29;11(532). pii: 11/532/eaap8113. doi: 10.1126/scisignal.aap8113. PubMed
FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex. Kunzel U, Grieve AG, Meng Y, Sieber B, Cowley SA, Freeman M. Elife. 2018 Jun 13;7. pii: 35012. doi: 10.7554/eLife.35012. PubMed
Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production. Zhang J, Lan Y, Li MY, Lamers MM, Fusade-Boyer M, Klemm E, Thiele C, Ashour J, Sanyal S. Cell Host Microbe. 2018 Jun 13;23(6):819-831.e5. doi: 10.1016/j.chom.2018.05.005. PubMed
Interaction of heterotrimeric kinesin-II with IFT-B-connecting tetramer is crucial for ciliogenesis. Funabashi T, Katoh Y, Okazaki M, Sugawa M, Nakayama K. J Cell Biol. 2018 Aug 6;217(8):2867-2876. doi: 10.1083/jcb.201801039. Epub 2018 Jun 14. PubMed
Transbilayer phospholipid movement facilitates the translocation of annexin across membranes. Stewart SE, Ashkenazi A, Williamson A, Rubinsztein DC, Moreau K. J Cell Sci. 2018 Jul 19;131(14). pii: jcs.217034. doi: 10.1242/jcs.217034. PubMed
The PARP1-Siah1 Axis Controls HIV-1 Transcription and Expression of Siah1 Substrates. Yu D, Liu R, Yang G, Zhou Q. Cell Rep. 2018 Jun 26;23(13):3741-3749. doi: 10.1016/j.celrep.2018.05.084. PubMed
Induction of innate immune memory via microRNA targeting of chromatin remodelling factors. Seeley JJ, Baker RG, Mohamed G, Bruns T, Hayden MS, Deshmukh SD, Freedberg DE, Ghosh S. Nature. 2018 Jul;559(7712):114-119. doi: 10.1038/s41586-018-0253-5. Epub 2018 Jun 27. PubMed
Three classes of response elements for human PRC2 and MLL1/2-Trithorax complexes. Du J, Kirk B, Zeng J, Ma J, Wang Q. Nucleic Acids Res. 2018 Sep 28;46(17):8848-8864. doi: 10.1093/nar/gky595. PubMed
A SIRT1-centered circuitry regulates breast cancer stemness and metastasis. Shi L, Tang X, Qian M, Liu Z, Meng F, Fu L, Wang Z, Zhu WG, Huang JD, Zhou Z, Liu B. Oncogene. 2018 Dec;37(49):6299-6315. doi: 10.1038/s41388-018-0370-5. Epub 2018 Jul 23. PubMed
CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A. Leung JWC, Emery LE, Miller KM. Methods Mol Biol. 2018;1832:255-269. doi: 10.1007/978-1-4939-8663-7_14. PubMed
Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells. Barnes J, Salas F, Mokhtari R, Dolstra H, Pedrosa E, Lachman HM. Mol Autism. 2018 Aug 15;9:44. doi: 10.1186/s13229-018-0227-3. eCollection 2018. PubMed
On the relation between filament density, force generation, and protrusion rate in mesenchymal cell motility. Dolati S, Kage F, Mueller J, Musken M, Kirchner M, Dittmar G, Sixt M, Rottner K, Falcke M. Mol Biol Cell. 2018 Nov 1;29(22):2674-2686. doi: 10.1091/mbc.E18-02-0082. Epub 2018 Aug 29. PubMed
A feed forward loop enforces YAP/TAZ signaling during tumorigenesis. Gill MK, Christova T, Zhang YY, Gregorieff A, Zhang L, Narimatsu M, Song S, Xiong S, Couzens AL, Tong J, Krieger JR, Moran MF, Zlotta AR, van der Kwast TH, Gingras AC, Sicheri F, Wrana JL, Attisano L. Nat Commun. 2018 Aug 29;9(1):3510. doi: 10.1038/s41467-018-05939-2. PubMed
CRL4(AMBRA1) targets Elongin C for ubiquitination and degradation to modulate CRL5 signaling. Chen SH, Jang GM, Huttenhain R, Gordon DE, Du D, Newton BW, Johnson JR, Hiatt J, Hultquist JF, Johnson TL, Liu YL, Burton LA, Ye J, Reichermeier KM, Stroud RM, Marson A, Debnath J, Gross JD, Krogan NJ. EMBO J. 2018 Sep 14;37(18). pii: embj.201797508. doi: 10.15252/embj.201797508. Epub 2018 Aug 30. PubMed
DROSHA Knockout Leads to Enhancement of Viral Titers for Vectors Encoding miRNA-Adapted shRNAs. Park HH, Triboulet R, Bentler M, Guda S, Du P, Xu H, Gregory RI, Brendel C, Williams DA. Mol Ther Nucleic Acids. 2018 Sep 7;12:591-599. doi: 10.1016/j.omtn.2018.07.002. Epub 2018 Jul 9. PubMed
Transcription coactivator Cited1 acts as an inducer of trophoblast-like state from mouse embryonic stem cells through the activation of BMP signaling. Xu Y, Luo X, Fang Z, Zheng X, Zeng Y, Zhu C, Gu J, Tang F, Hu Y, Hu G, Jin Y, Li H. Cell Death Dis. 2018 Sep 11;9(9):924. doi: 10.1038/s41419-018-0991-1. PubMed
Control of directionality of chromatin folding for the inter- and intra-domain contacts at the Tfap2c-Bmp7 locus. Tsujimura T, Takase O, Yoshikawa M, Sano E, Hayashi M, Takato T, Toyoda A, Okano H, Hishikawa K. Epigenetics Chromatin. 2018 Sep 14;11(1):51. doi: 10.1186/s13072-018-0221-1. PubMed
Generation of PTENknockout (/) murine prostate cancer cells using the CRISPR/Cas9 system and comprehensive gene expression profiling. Takao A, Yoshikawa K, Karnan S, Ota A, Uemura H, De Velasco MA, Kura Y, Suzuki S, Ueda R, Nishino T, Hosokawa Y. Oncol Rep. 2018 Nov;40(5):2455-2466. doi: 10.3892/or.2018.6683. Epub 2018 Sep 5. PubMed
Manifold roles of beta-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9. Luttrell LM, Wang J, Plouffe B, Smith JS, Yamani L, Kaur S, Jean-Charles PY, Gauthier C, Lee MH, Pani B, Kim J, Ahn S, Rajagopal S, Reiter E, Bouvier M, Shenoy SK, Laporte SA, Rockman HA, Lefkowitz RJ. Sci Signal. 2018 Sep 25;11(549). pii: 11/549/eaat7650. doi: 10.1126/scisignal.aat7650. PubMed
Inhibition of HBV Expression in HBV Transgenic Mice Using AAV-Delivered CRISPR-SaCas9. Li H, Sheng C, Liu H, Wang S, Zhao J, Yang L, Jia L, Li P, Wang L, Xie J, Xu D, Sun Y, Qiu S, Song H. Front Immunol. 2018 Sep 11;9:2080. doi: 10.3389/fimmu.2018.02080. eCollection 2018. PubMed
Expression and significance of Cystatin-C in clear cell renal cell carcinoma. Guo K, Chen Q, He X, Yao K, Li Z, Liu Z, Chen J, Liu Z, Guo C, Lu J, Wu C, Li W, Wang Q, Chen P, Lu W, Wang Y, Han H, Cao Y, Guo S. Biomed Pharmacother. 2018 Nov;107:1237-1245. doi: 10.1016/j.biopha.2018.08.083. Epub 2018 Aug 29. PubMed
Critical role for cholesterol in Lassa fever virus entry identified by a novel small molecule inhibitor targeting the viral receptor LAMP1. Wang MK, Ren T, Liu H, Lim SY, Lee K, Honko A, Zhou H, Dyall J, Hensley L, Gartin AK, Cunningham JM. PLoS Pathog. 2018 Sep 28;14(9):e1007322. doi: 10.1371/journal.ppat.1007322. eCollection 2018 Sep. PubMed
Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels. Iida R, Ueki M, Yasuda T. Oxid Med Cell Longev. 2018 Sep 17;2018:6956414. doi: 10.1155/2018/6956414. eCollection 2018. PubMed
Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. Li W, Yue F, Dai Y, Shi B, Xu G, Jiang X, Zhou X, Pfeifer GP, Liu L. Cell Death Differ. 2018 Oct 12. pii: 10.1038/s41418-018-0211-7. doi: 10.1038/s41418-018-0211-7. PubMed
Identification of UHRF2 as a novel DNA interstrand crosslink sensor protein. Motnenko A, Liang CC, Yang D, Lopez-Martinez D, Yoshikawa Y, Zhan B, Ward KE, Tian J, Haas W, Spingardi P, Kessler BM, Kriaucionis S, Gygi SP, Cohn MA. PLoS Genet. 2018 Oct 18;14(10):e1007643. doi: 10.1371/journal.pgen.1007643. eCollection 2018 Oct. PubMed
Gesicle-Mediated Delivery of CRISPR/Cas9 Ribonucleoprotein Complex for Inactivating the HIV Provirus. Campbell LA, Coke LM, Richie CT, Fortuno LV, Park AY, Harvey BK. Mol Ther. 2019 Jan 2;27(1):151-163. doi: 10.1016/j.ymthe.2018.10.002. Epub 2018 Oct 11. PubMed
NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells. Kloet SL, Karemaker ID, van Voorthuijsen L, Lindeboom RGH, Baltissen MP, Edupuganti RR, Poramba-Liyanage DW, Jansen PWTC, Vermeulen M. Nat Commun. 2018 Nov 2;9(1):4588. doi: 10.1038/s41467-018-07063-7. PubMed
Distinct Interaction Sites of Rac GTPase with WAVE Regulatory Complex Have Non-redundant Functions in Vivo. Schaks M, Singh SP, Kage F, Thomason P, Klunemann T, Steffen A, Blankenfeldt W, Stradal TE, Insall RH, Rottner K. Curr Biol. 2018 Nov 19;28(22):3674-3684.e6. doi: 10.1016/j.cub.2018.10.002. Epub 2018 Nov 1. PubMed
Loss of VGLL4 suppresses tumor PD-L1 expression and immune evasion. Wu A, Wu Q, Deng Y, Liu Y, Lu J, Liu L, Li X, Liao C, Zhao B, Song H. EMBO J. 2019 Jan 3;38(1). pii: embj.201899506. doi: 10.15252/embj.201899506. Epub 2018 Nov 5. PubMed
Genetic Analysis Reveals AMPK Is Required to Support Tumor Growth in Murine Kras-Dependent Lung Cancer Models. Eichner LJ, Brun SN, Herzig S, Young NP, Curtis SD, Shackelford DB, Shokhirev MN, Leblanc M, Vera LI, Hutchins A, Ross DS, Shaw RJ, Svensson RU. Cell Metab. 2019 Feb 5;29(2):285-302.e7. doi: 10.1016/j.cmet.2018.10.005. Epub 2018 Nov 8. PubMed
REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice. Gontan C, Mira-Bontenbal H, Magaraki A, Dupont C, Barakat TS, Rentmeester E, Demmers J, Gribnau J. Nat Commun. 2018 Nov 12;9(1):4752. doi: 10.1038/s41467-018-07060-w. PubMed
Two Interlinked Bistable Switches Govern Mitotic Control in Mammalian Cells. Rata S, Suarez Peredo Rodriguez MF, Joseph S, Peter N, Echegaray Iturra F, Yang F, Madzvamuse A, Ruppert JG, Samejima K, Platani M, Alvarez-Fernandez M, Malumbres M, Earnshaw WC, Novak B, Hochegger H. Curr Biol. 2018 Dec 3;28(23):3824-3832.e6. doi: 10.1016/j.cub.2018.09.059. Epub 2018 Nov 15. PubMed
Transcription of the NKG2D ligand MICA is suppressed by the IRE1/XBP1 pathway of the unfolded protein response through the regulation of E2F1. Obiedat A, Seidel E, Mahameed M, Berhani O, Tsukerman P, Voutetakis K, Chatziioannou A, McMahon M, Avril T, Chevet E, Mandelboim O, Tirosh B. FASEB J. 2019 Mar;33(3):3481-3495. doi: 10.1096/fj.201801350RR. Epub 2018 Nov 19. PubMed
ROBO4 variants predispose individuals to bicuspid aortic valve and thoracic aortic aneurysm. Gould RA, Aziz H, Woods CE, Seman-Senderos MA, Sparks E, Preuss C, Wunnemann F, Bedja D, Moats CR, McClymont SA, Rose R, Sobreira N, Ling H, MacCarrick G, Kumar AA, Luyckx I, Cannaerts E, Verstraeten A, Bjork HM, Lehsau AC, Jaskula-Ranga V, Lauridsen H, Shah AA, Bennett CL, Ellinor PT, Lin H, Isselbacher EM, Lino Cardenas CL, Butcher JT, Hughes GC, Lindsay ME, Mertens L, Franco-Cereceda A, Verhagen JMA, Wessels M, Mohamed SA, Eriksson P, Mital S, Van Laer L, Loeys BL, Andelfinger G, McCallion AS, Dietz HC. Nat Genet. 2019 Jan;51(1):42-50. doi: 10.1038/s41588-018-0265-y. Epub 2018 Nov 19. PubMed
C11ORF74 interacts with the IFT-A complex and participates in ciliary BBSome localization. Takahara M, Kunii M, Nakamura K, Harada A, Hirano T, Katoh Y, Nakayama K. J Biochem. 2019 Mar 1;165(3):257-267. doi: 10.1093/jb/mvy100. PubMed
Pyruvate dehydrogenase kinase 1 and carbonic anhydrase IX targeting in hypoxic tumors. Kery M, Oravcova N, Radenkovic S, Iuliano F, Tomaskova J, Golias T. Neoplasma. 2018 Dec 10;66(1):63-72. doi: 10.4149/neo_2018_180531N357. PubMed
A Well-Controlled BioID Design for Endogenous Bait Proteins. Vandemoortele G, De Sutter D, Moliere A, Pauwels J, Gevaert K, Eyckerman S. J Proteome Res. 2018 Dec 10. doi: 10.1021/acs.jproteome.8b00367. PubMed
LncRNA TP73-AS1 promoted the progression of lung adenocarcinoma via PI3K/AKT pathway. Liu C, Ren L, Deng J, Wang S. Biosci Rep. 2019 Jan 11;39(1). pii: BSR20180999. doi: 10.1042/BSR20180999. Print 2019 Jan 31. PubMed
The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. Menzies SA, Volkmar N, van den Boomen DJ, Timms RT, Dickson AS, Nathan JA, Lehner PJ. Elife. 2018 Dec 13;7. pii: 40009. doi: 10.7554/eLife.40009. PubMed
HMCES Maintains Genome Integrity by Shielding Abasic Sites in Single-Strand DNA. Mohni KN, Wessel SR, Zhao R, Wojciechowski AC, Luzwick JW, Layden H, Eichman BF, Thompson PS, Mehta KPM, Cortez D. Cell. 2019 Jan 10;176(1-2):144-153.e13. doi: 10.1016/j.cell.2018.10.055. Epub 2018 Dec 13. PubMed
The Hippo Pathway Regulates Caveolae Expression and Mediates Flow Response via Caveolae. Rausch V, Bostrom JR, Park J, Bravo IR, Feng Y, Hay DC, Link BA, Hansen CG. Curr Biol. 2019 Jan 21;29(2):242-255.e6. doi: 10.1016/j.cub.2018.11.066. Epub 2018 Dec 27. PubMed
CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification. Oakes BL, Fellmann C, Rishi H, Taylor KL, Ren SM, Nadler DC, Yokoo R, Arkin AP, Doudna JA, Savage DF. Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052. PubMed

Associated Plasmids

HIF-1alpha and HIF-2alpha Differently Regulate the Radiation Sensitivity of NSCLC Cells. Moreno Roig E, Groot AJ, Yaromina A, Hendrickx TC, Barbeau LMO, Giuranno L, Dams G, Ient J, Olivo Pimentel V, van Gisbergen MW, Dubois LJ, Vooijs MA. Cells. 2019 Jan 12;8(1). pii: cells8010045. doi: 10.3390/cells8010045. PubMed
LEADeR role of miR-205 host gene as long noncoding RNA in prostate basal cell differentiation. Profumo V, Forte B, Percio S, Rotundo F, Doldi V, Ferrari E, Fenderico N, Dugo M, Romagnoli D, Benelli M, Valdagni R, Dolfini D, Zaffaroni N, Gandellini P. Nat Commun. 2019 Jan 18;10(1):307. doi: 10.1038/s41467-018-08153-2. PubMed
Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells. Fenderico N, van Scherpenzeel RC, Goldflam M, Proverbio D, Jordens I, Kralj T, Stryeck S, Bass TZ, Hermans G, Ullman C, Aastrup T, Gros P, Maurice MM. Nat Commun. 2019 Jan 21;10(1):365. doi: 10.1038/s41467-018-08172-z. PubMed
CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization. Hayward D, Alfonso-Perez T, Cundell MJ, Hopkins M, Holder J, Bancroft J, Hutter LH, Novak B, Barr FA, Gruneberg U. J Cell Biol. 2019 Apr 1;218(4):1182-1199. doi: 10.1083/jcb.201808014. Epub 2019 Jan 23. PubMed
Exogenous Monounsaturated Fatty Acids Promote a Ferroptosis-Resistant Cell State. Magtanong L, Ko PJ, To M, Cao JY, Forcina GC, Tarangelo A, Ward CC, Cho K, Patti GJ, Nomura DK, Olzmann JA, Dixon SJ. Cell Chem Biol. 2019 Mar 21;26(3):420-432.e9. doi: 10.1016/j.chembiol.2018.11.016. Epub 2019 Jan 24. PubMed
Robust and efficient knock-in in embryonic stem cells and early-stage embryos of the common marmoset using the CRISPR-Cas9 system. Yoshimatsu S, Okahara J, Sone T, Takeda Y, Nakamura M, Sasaki E, Kishi N, Shiozawa S, Okano H. Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1038/s41598-018-37990-w. PubMed

Associated Plasmids

Analysis of Transcriptional Regulation in Bone Cells. Jin H, Sophocleous A, Azfer A, Ralston SH. Methods Mol Biol. 2019;1914:145-167. doi: 10.1007/978-1-4939-8997-3_8. PubMed
Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility. Yeshaw WM, van der Zwaag M, Pinto F, Lahaye LL, Faber AI, Gomez-Sanchez R, Dolga AM, Poland C, Monaco AP, van IJzendoorn SC, Grzeschik NA, Velayos-Baeza A, Sibon OC. Elife. 2019 Feb 11;8. pii: 43561. doi: 10.7554/eLife.43561. PubMed
SLFN11 can sensitize tumor cells towards IFN-gamma-mediated T cell killing. Mezzadra R, de Bruijn M, Jae LT, Gomez-Eerland R, Duursma A, Scheeren FA, Brummelkamp TR, Schumacher TN. PLoS One. 2019 Feb 12;14(2):e0212053. doi: 10.1371/journal.pone.0212053. eCollection 2019. PubMed
Quantitative phosphoproteomics reveals mitotic function of the ATR activator ETAA1. Bass TE, Cortez D. J Cell Biol. 2019 Apr 1;218(4):1235-1249. doi: 10.1083/jcb.201810058. Epub 2019 Feb 12. PubMed
Statins and Histone Deacetylase Inhibitors Affect Lamin A/C - Histone Deacetylase 2 Interaction in Human Cells. Mattioli E, Andrenacci D, Mai A, Valente S, Robijns J, De Vos WH, Capanni C, Lattanzi G. Front Cell Dev Biol. 2019 Jan 31;7:6. doi: 10.3389/fcell.2019.00006. eCollection 2019. PubMed
Loss of Emp2 compromises cardiogenic differentiation in mouse embryonic stem cells. Liu Y, Dakou E, Meng Y, Leyns L. Biochem Biophys Res Commun. 2019 Feb 14. pii: S0006-291X(19)30235-9. doi: 10.1016/j.bbrc.2019.02.048. PubMed

Associated Plasmids

Prolyl isomerization of FAAP20 catalyzed by PIN1 regulates the Fanconi anemia pathway. Wang J, Chan B, Tong M, Paung Y, Jo U, Martin D, Seeliger M, Haley J, Kim H. PLoS Genet. 2019 Feb 21;15(2):e1007983. doi: 10.1371/journal.pgen.1007983. eCollection 2019 Feb. PubMed
Differential genomic destabilisation in human cells with pathogenic MSH2 mutations introduced by genome editing. Hayashida G, Shioi S, Hidaka K, Fujikane R, Hidaka M, Tsurimoto T, Tsuzuki T, Oda S, Nakatsu Y. Exp Cell Res. 2019 Apr 15;377(1-2):24-35. doi: 10.1016/j.yexcr.2019.02.020. Epub 2019 Feb 22. PubMed
Human Fis1 regulates mitochondrial dynamics through inhibition of the fusion machinery. Yu R, Jin SB, Lendahl U, Nister M, Zhao J. EMBO J. 2019 Apr 15;38(8). pii: embj.201899748. doi: 10.15252/embj.201899748. Epub 2019 Mar 6. PubMed
CHD7 promotes glioblastoma cell motility and invasiveness through transcriptional modulation of an invasion signature. Machado RAC, Schneider H, DeOcesano-Pereira C, Lichtenstein F, Andrade F, Fujita A, Trombetta-Lima M, Weller M, Bowman-Colin C, Sogayar MC. Sci Rep. 2019 Mar 8;9(1):3952. doi: 10.1038/s41598-019-39564-w. PubMed
A complex between DYRK1A and DCAF7 phosphorylates the C-terminal domain of RNA polymerase II to promote myogenesis. Yu D, Cattoglio C, Xue Y, Zhou Q. Nucleic Acids Res. 2019 May 21;47(9):4462-4475. doi: 10.1093/nar/gkz162. PubMed
PKM2 coordinates glycolysis with mitochondrial fusion and oxidative phosphorylation. Li T, Han J, Jia L, Hu X, Chen L, Wang Y. Protein Cell. 2019 Aug;10(8):583-594. doi: 10.1007/s13238-019-0618-z. Epub 2019 Mar 18. PubMed
Construction of non-canonical PAM-targeting adenosine base editors by restriction enzyme-free DNA cloning using CRISPR-Cas9. Jeong YK, Yu J, Bae S. Sci Rep. 2019 Mar 20;9(1):4939. doi: 10.1038/s41598-019-41356-1. PubMed
Oncogenic PIK3CA promotes cellular stemness in an allele dose-dependent manner. Madsen RR, Knox RG, Pearce W, Lopez S, Mahler-Araujo B, McGranahan N, Vanhaesebroeck B, Semple RK. Proc Natl Acad Sci U S A. 2019 Apr 23;116(17):8380-8389. doi: 10.1073/pnas.1821093116. Epub 2019 Apr 4. PubMed
A role for Rab11 in the homeostasis of the endosome-lysosomal pathway. Zulkefli KL, Houghton FJ, Gosavi P, Gleeson PA. Exp Cell Res. 2019 Jul 1;380(1):55-68. doi: 10.1016/j.yexcr.2019.04.010. Epub 2019 Apr 11. PubMed
LC8/DYNLL1 is a 53BP1 effector and regulates checkpoint activation. West KL, Kelliher JL, Xu Z, An L, Reed MR, Eoff RL, Wang J, Huen MSY, Leung JWC. Nucleic Acids Res. 2019 Jul 9;47(12):6236-6249. doi: 10.1093/nar/gkz263. PubMed
Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns. Alateeq S, Ovchinnikov D, Tracey T, Whitworth D, Al-Rubaish A, Al-Ali A, Wolvetang E. APL Bioeng. 2018 Dec 3;2(4):046103. doi: 10.1063/1.5048625. eCollection 2018 Dec. PubMed
GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor. Jewell JL, Fu V, Hong AW, Yu FX, Meng D, Melick CH, Wang H, Lam WM, Yuan HX, Taylor SS, Guan KL. Elife. 2019 May 21;8. pii: 43038. doi: 10.7554/eLife.43038. PubMed
The BBSome in POMC and AgRP Neurons Is Necessary for Body Weight Regulation and Sorting of Metabolic Receptors. Guo DF, Lin Z, Wu Y, Searby C, Thedens DR, Richerson GB, Usachev YM, Grobe JL, Sheffield VC, Rahmouni K. Diabetes. 2019 Aug;68(8):1591-1603. doi: 10.2337/db18-1088. Epub 2019 May 24. PubMed
The bipartite TAD organization of the X-inactivation center ensures opposing developmental regulation of Tsix and Xist. van Bemmel JG, Galupa R, Gard C, Servant N, Picard C, Davies J, Szempruch AJ, Zhan Y, Zylicz JJ, Nora EP, Lameiras S, de Wit E, Gentien D, Baulande S, Giorgetti L, Guttman M, Hughes JR, Higgs DR, Gribnau J, Heard E. Nat Genet. 2019 Jun;51(6):1024-1034. doi: 10.1038/s41588-019-0412-0. Epub 2019 May 27. PubMed
RB1 Deletion in Retinoblastoma Protein Pathway-Disrupted Cells Results in DNA Damage and Cancer Progression. Marshall AE, Roes MV, Passos DT, DeWeerd MC, Chaikovsky AC, Sage J, Howlett CJ, Dick FA. Mol Cell Biol. 2019 Jul 29;39(16). pii: MCB.00105-19. doi: 10.1128/MCB.00105-19. Print 2019 Aug 15. PubMed
Involvement of Interferon Regulatory Factor 7 in Nicotine's Suppression of Antiviral Immune Responses. Han H, Huang W, Du W, Shen Q, Yang Z, Li MD, Chang SL. J Neuroimmune Pharmacol. 2019 Jun 1. pii: 10.1007/s11481-019-09845-2. doi: 10.1007/s11481-019-09845-2. PubMed
Phosphorylation of FANCD2 Inhibits the FANCD2/FANCI Complex and Suppresses the Fanconi Anemia Pathway in the Absence of DNA Damage. Lopez-Martinez D, Kupculak M, Yang D, Yoshikawa Y, Liang CC, Wu R, Gygi SP, Cohn MA. Cell Rep. 2019 Jun 4;27(10):2990-3005.e5. doi: 10.1016/j.celrep.2019.05.003. PubMed
ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint. Yuan F, Jin X, Li D, Song Y, Zhang N, Yang X, Wang L, Zhu WG, Tian C, Zhao Y. Nucleic Acids Res. 2019 Sep 5;47(15):8096-8110. doi: 10.1093/nar/gkz602. PubMed
Acetylation of BMAL1 by TIP60 controls BRD4-P-TEFb recruitment to circadian promoters. Petkau N, Budak H, Zhou X, Oster H, Eichele G. Elife. 2019 Jul 11;8. pii: 43235. doi: 10.7554/eLife.43235. PubMed
Toxoplasma GRA15 Activates the NF-kappaB Pathway through Interactions with TNF Receptor-Associated Factors. Sangare LO, Yang N, Konstantinou EK, Lu D, Mukhopadhyay D, Young LH, Saeij JPJ. mBio. 2019 Jul 16;10(4). pii: mBio.00808-19. doi: 10.1128/mBio.00808-19. PubMed
The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection. Moskovskich A, Goldmann U, Kartnig F, Lindinger S, Konecka J, Fiume G, Girardi E, Superti-Furga G. Sci Rep. 2019 Jul 18;9(1):10471. doi: 10.1038/s41598-019-46952-9. PubMed
Conditional deletion of glucocorticoid receptors in rat brain results in sex-specific deficits in fear and coping behaviors. Scheimann JR, Moloney RD, Mahbod P, Morano RL, Fitzgerald M, Hoskins O, Packard BA, Cotella EM, Hu YC, Herman JP. Elife. 2019 Jul 22;8. pii: 44672. doi: 10.7554/eLife.44672. PubMed
SMYD2 Drives Mesendodermal Differentiation of Human Embryonic Stem Cells Through Mediating the Transcriptional Activation of Key Mesendodermal Genes. Bai HJ, Zhang P, Ma L, Liang H, Wei G, Yang HT. Stem Cells. 2019 Nov;37(11):1401-1415. doi: 10.1002/stem.3068. Epub 2019 Aug 12. PubMed
Gene-edited murine cell lines for propagation of chronic wasting disease prions. Walia R, Ho CC, Lee C, Gilch S, Schatzl HM. Sci Rep. 2019 Aug 1;9(1):11151. doi: 10.1038/s41598-019-47629-z. PubMed
Autokinase Activity of Casein Kinase 1 delta/epsilon Governs the Period of Mammalian Circadian Rhythms. Guo G, Wang K, Hu SS, Tian T, Liu P, Mori T, Chen P, Johnson CH, Qin X. J Biol Rhythms. 2019 Aug 8:748730419865406. doi: 10.1177/0748730419865406. PubMed
The synthesis of branched-chain fatty acids is limited by enzymatic decarboxylation of ethyl- and methylmalonyl-CoA. Dewulf JP, Gerin I, Rider MH, Veiga-da-Cunha M, Van Schaftingen E, Bommer GT. Biochem J. 2019 Aug 30;476(16):2427-2447. doi: 10.1042/BCJ20190500. PubMed
Single-Cell Analysis of Multiple Steps of Dynamic NF-kappaB Regulation in Interleukin-1alpha-Triggered Tumor Cells Using Proximity Ligation Assays. Mayr-Buro C, Schlereth E, Beuerlein K, Tenekeci U, Meier-Soelch J, Schmitz ML, Kracht M. Cancers (Basel). 2019 Aug 16;11(8). pii: cancers11081199. doi: 10.3390/cancers11081199. PubMed
Regulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing system. Kang JG, Park JS, Ko JH, Kim YS. Sci Rep. 2019 Aug 19;9(1):11960. doi: 10.1038/s41598-019-48130-3. PubMed
Attenuation of the Innate Immune Response against Viral Infection Due to ZNF598-Promoted Binding of FAT10 to RIG-I. Wang G, Kouwaki T, Okamoto M, Oshiumi H. Cell Rep. 2019 Aug 20;28(8):1961-1970.e4. doi: 10.1016/j.celrep.2019.07.081. PubMed
A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology. Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, Nakamura M, Sasaki E, Shiozawa S, Okano H. PLoS One. 2019 Aug 27;14(8):e0221164. doi: 10.1371/journal.pone.0221164. eCollection 2019. PubMed

Associated Plasmids

Transcriptomic analyses of gene expression by CRISPR knockout of miR-214 in cervical cancer cells. Sen P, Ghosal S, Hazra R, Arega S, Mohanty R, Kulkarni KK, Budhwar R, Ganguly N. Genomics. 2020 Mar;112(2):1490-1499. doi: 10.1016/j.ygeno.2019.08.020. Epub 2019 Aug 26. PubMed
Transcriptional Responses to IFN-gamma Require Mediator Kinase-Dependent Pause Release and Mechanistically Distinct CDK8 and CDK19 Functions. Steinparzer I, Sedlyarov V, Rubin JD, Eislmayr K, Galbraith MD, Levandowski CB, Vcelkova T, Sneezum L, Wascher F, Amman F, Kleinova R, Bender H, Andrysik Z, Espinosa JM, Superti-Furga G, Dowell RD, Taatjes DJ, Kovarik P. Mol Cell. 2019 Nov 7;76(3):485-499.e8. doi: 10.1016/j.molcel.2019.07.034. Epub 2019 Sep 5. PubMed
Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity. Gemble S, Simon A, Pennetier C, Dumont M, Herve S, Meitinger F, Oegema K, Rodriguez R, Almouzni G, Fachinetti D, Basto R. Curr Biol. 2019 Sep 23;29(18):3072-3080.e5. doi: 10.1016/j.cub.2019.07.052. Epub 2019 Sep 5. PubMed
Telomere dysfunction impairs epidermal stem cell specification and differentiation by disrupting BMP/pSmad/P63 signaling. Liu N, Yin Y, Wang H, Zhou Z, Sheng X, Fu H, Guo R, Wang H, Yang J, Gong P, Ning W, Ju Z, Liu Y, Liu L. PLoS Genet. 2019 Sep 13;15(9):e1008368. doi: 10.1371/journal.pgen.1008368. eCollection 2019 Sep. PubMed
Mouse Tryptase Gene Expression is Coordinately Regulated by GATA1 and GATA2 in Bone Marrow-Derived Mast Cells. Ohneda K, Ohmori S, Yamamoto M. Int J Mol Sci. 2019 Sep 17;20(18). pii: ijms20184603. doi: 10.3390/ijms20184603. PubMed
Human GLB1 knockout cerebral organoids: A model system for testing AAV9-mediated GLB1 gene therapy for reducing GM1 ganglioside storage in GM1 gangliosidosis. Latour YL, Yoon R, Thomas SE, Grant C, Li C, Sena-Esteves M, Allende ML, Proia RL, Tifft CJ. Mol Genet Metab Rep. 2019 Sep 11;21:100513. doi: 10.1016/j.ymgmr.2019.100513. eCollection 2019 Dec. PubMed
PINK1/Parkin Influences Cell Cycle by Sequestering TBK1 at Damaged Mitochondria, Inhibiting Mitosis. Sarraf SA, Sideris DP, Giagtzoglou N, Ni L, Kankel MW, Sen A, Bochicchio LE, Huang CH, Nussenzweig SC, Worley SH, Morton PD, Artavanis-Tsakonas S, Youle RJ, Pickrell AM. Cell Rep. 2019 Oct 1;29(1):225-235.e5. doi: 10.1016/j.celrep.2019.08.085. PubMed
Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Laflamme C, McKeever PM, Kumar R, Schwartz J, Kolahdouzan M, Chen CX, You Z, Benaliouad F, Gileadi O, McBride HM, Durcan TM, Edwards AM, Healy LM, Robertson J, McPherson PS. Elife. 2019 Oct 15;8. pii: 48363. doi: 10.7554/eLife.48363. PubMed
Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids. Chhabra S, Liu L, Goh R, Kong X, Warmflash A. PLoS Biol. 2019 Oct 15;17(10):e3000498. doi: 10.1371/journal.pbio.3000498. eCollection 2019 Oct. PubMed
The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis. Bersuker K, Hendricks JM, Li Z, Magtanong L, Ford B, Tang PH, Roberts MA, Tong B, Maimone TJ, Zoncu R, Bassik MC, Nomura DK, Dixon SJ, Olzmann JA. Nature. 2019 Nov;575(7784):688-692. doi: 10.1038/s41586-019-1705-2. Epub 2019 Oct 21. PubMed
53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination. Callen E, Zong D, Wu W, Wong N, Stanlie A, Ishikawa M, Pavani R, Dumitrache LC, Byrum AK, Mendez-Dorantes C, Martinez P, Canela A, Maman Y, Day A, Kruhlak MJ, Blasco MA, Stark JM, Mosammaparast N, McKinnon PJ, Nussenzweig A. Mol Cell. 2020 Jan 2;77(1):26-38.e7. doi: 10.1016/j.molcel.2019.09.024. Epub 2019 Oct 22. PubMed
Pyridoxamine-phosphate oxidases and pyridoxamine-phosphate oxidase-related proteins catalyze the oxidation of 6-NAD(P)H to NAD(P). Marbaix AY, Chehade G, Noel G, Morsomme P, Vertommen D, Bommer GT, Van Schaftingen E. Biochem J. 2019 Oct 30;476(20):3033-3052. doi: 10.1042/BCJ20190602. PubMed
Distinct IL-1alpha-responsive enhancers promote acute and coordinated changes in chromatin topology in a hierarchical manner. Weiterer SS, Meier-Soelch J, Georgomanolis T, Mizi A, Beyerlein A, Weiser H, Brant L, Mayr-Buro C, Jurida L, Beuerlein K, Muller H, Weber A, Tenekeci U, Dittrich-Breiholz O, Bartkuhn M, Nist A, Stiewe T, van IJcken WF, Riedlinger T, Schmitz ML, Papantonis A, Kracht M. EMBO J. 2020 Jan 2;39(1):e101533. doi: 10.15252/embj.2019101533. Epub 2019 Nov 7. PubMed
The role of CXC receptors signaling in early stages of mouse embryonic stem cell differentiation. Kowalski K, Brzoska E, Ciemerych MA. Stem Cell Res. 2019 Dec;41:101636. doi: 10.1016/j.scr.2019.101636. Epub 2019 Oct 31. PubMed
Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex. Naito T, Ercan B, Krshnan L, Triebl A, Koh DHZ, Wei FY, Tomizawa K, Torta FT, Wenk MR, Saheki Y. Elife. 2019 Nov 14;8. pii: 51401. doi: 10.7554/eLife.51401. PubMed
Inhibitor of Growth 4 (ING4) is a positive regulator of rRNA synthesis. Trinh DA, Shirakawa R, Kimura T, Sakata N, Goto K, Horiuchi H. Sci Rep. 2019 Nov 21;9(1):17235. doi: 10.1038/s41598-019-53767-1. PubMed
Potent Antitumor Activity of Liposomal Irinotecan in an Organoid- and CRISPR-Cas9-Based Murine Model of Gallbladder Cancer. Erlangga Z, Wolff K, Poth T, Peltzer A, Nahnsen S, Spielberg S, Timrott K, Woller N, Kuhnel F, Manns MP, Saborowski A, Vogel A, Saborowski M. Cancers (Basel). 2019 Nov 29;11(12). pii: cancers11121904. doi: 10.3390/cancers11121904. PubMed
Lack of the multidrug transporter MRP4/ABCC4 defines the PEL-negative blood group and impairs platelet aggregation. Azouzi S, Mikdar M, Hermand P, Gautier EF, Salnot V, Willemetz A, Nicolas G, Vrignaud C, Raneri A, Mayeux P, Bole-Feysot C, Nitschke P, Cartron JP, Colin Y, Hermine O, Jedlitschky G, Cloutier M, Constanzo-Yanez J, Ethier C, Robitaille N, St-Louis M, Le Van Kim C, Peyrard T. Blood. 2020 Feb 6;135(6):441-448. doi: 10.1182/blood.2019002320. PubMed
Identification of ncRNA-Mediated Functions of Nucleus-Localized miR-320 in Cardiomyocytes. Li H, Zhan J, Zhao Y, Fan J, Yuan S, Yin Z, Dai B, Chen C, Wang DW. Mol Ther Nucleic Acids. 2019 Nov 18;19:132-143. doi: 10.1016/j.omtn.2019.11.006. PubMed
The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Leidal AM, Huang HH, Marsh T, Solvik T, Zhang D, Ye J, Kai F, Goldsmith J, Liu JY, Huang YH, Monkkonen T, Vlahakis A, Huang EJ, Goodarzi H, Yu L, Wiita AP, Debnath J. Nat Cell Biol. 2020 Feb;22(2):187-199. doi: 10.1038/s41556-019-0450-y. Epub 2020 Jan 13. PubMed
YB-3 substitutes YB-1 in global mRNA binding. Lyabin DN, Eliseeva IA, Smolin EA, Doronin AN, Budkina KS, Kulakovskiy IV, Ovchinnikov LP. RNA Biol. 2020 Apr;17(4):487-499. doi: 10.1080/15476286.2019.1710050. Epub 2020 Jan 16. PubMed
Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells. Rhodes JDP, Feldmann A, Hernandez-Rodriguez B, Diaz N, Brown JM, Fursova NA, Blackledge NP, Prathapan P, Dobrinic P, Huseyin MK, Szczurek A, Kruse K, Nasmyth KA, Buckle VJ, Vaquerizas JM, Klose RJ. Cell Rep. 2020 Jan 21;30(3):820-835.e10. doi: 10.1016/j.celrep.2019.12.057. PubMed
The mitochondrial HSP90 paralog TRAP1 forms an OXPHOS-regulated tetramer and is involved in mitochondrial metabolic homeostasis. Joshi A, Dai L, Liu Y, Lee J, Ghahhari NM, Segala G, Beebe K, Jenkins LM, Lyons GC, Bernasconi L, Tsai FTF, Agard DA, Neckers L, Picard D. BMC Biol. 2020 Jan 27;18(1):10. doi: 10.1186/s12915-020-0740-7. PubMed
The role of Mediator and Little Elongation Complex in transcription termination. Takahashi H, Ranjan A, Chen S, Suzuki H, Shibata M, Hirose T, Hirose H, Sasaki K, Abe R, Chen K, He Y, Zhang Y, Takigawa I, Tsukiyama T, Watanabe M, Fujii S, Iida M, Yamamoto J, Yamaguchi Y, Suzuki Y, Matsumoto M, Nakayama KI, Washburn MP, Saraf A, Florens L, Sato S, Tomomori-Sato C, Conaway RC, Conaway JW, Hatakeyama S. Nat Commun. 2020 Feb 26;11(1):1063. doi: 10.1038/s41467-020-14849-1. PubMed
Critical roles of phosphoinositides and NF2 in Hippo pathway regulation. Hong AW, Meng Z, Plouffe SW, Lin Z, Zhang M, Guan KL. Genes Dev. 2020 Apr 1;34(7-8):511-525. doi: 10.1101/gad.333435.119. Epub 2020 Feb 27. PubMed
Polycomb group RING finger protein 5 influences several developmental signaling pathways during the in vitro differentiation of mouse embryonic stem cells. Meng Y, Liu Y, Dakou E, Gutierrez GJ, Leyns L. Dev Growth Differ. 2020 May;62(4):232-242. doi: 10.1111/dgd.12659. Epub 2020 Apr 22. PubMed
Identification of the Repressive Domain of the Negative Circadian Clock Component CHRONO. Yang Y, Li N, Qiu J, Ge H, Qin X. Int J Mol Sci. 2020 Apr 2;21(7). pii: ijms21072469. doi: 10.3390/ijms21072469. PubMed
LPIAT1/MBOAT7 depletion increases triglyceride synthesis fueled by high phosphatidylinositol turnover. Tanaka Y, Shimanaka Y, Caddeo A, Kubo T, Mao Y, Kubota T, Kubota N, Yamauchi T, Mancina RM, Baselli G, Luukkonen P, Pihlajamaki J, Yki-Jarvinen H, Valenti L, Arai H, Romeo S, Kono N. Gut. 2021 Jan;70(1):180-193. doi: 10.1136/gutjnl-2020-320646. Epub 2020 Apr 6. PubMed
CRISPR/CAS9-mediated knockout of Abi1 inhibits p185(Bcr-Abl)-induced leukemogenesis and signal transduction to ERK and PI3K/Akt pathways. Faulkner J, Jiang P, Farris D, Walker R, Dai Z. J Hematol Oncol. 2020 Apr 10;13(1):34. doi: 10.1186/s13045-020-00867-5. PubMed
TDP2 suppresses genomic instability induced by androgens in the epithelial cells of prostate glands. Al Mahmud MR, Ishii K, Bernal-Lozano C, Delgado-Sainz I, Toi M, Akamatsu S, Fukumoto M, Watanabe M, Takeda S, Cortes-Ledesma F, Sasanuma H. Genes Cells. 2020 Jul;25(7):450-465. doi: 10.1111/gtc.12770. Epub 2020 May 5. PubMed
Nek2-mediated GAS2L1 phosphorylation and centrosome-linker disassembly induce centrosome disjunction. Au FKC, Hau BKT, Qi RZ. J Cell Biol. 2020 May 4;219(5). pii: 151657. doi: 10.1083/jcb.201909094. PubMed
Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration. Kim Y, Lee J, Seppala M, Cobourne MT, Kim SH. Nat Commun. 2020 Apr 24;11(1):1994. doi: 10.1038/s41467-020-15897-3. PubMed
Loss of calpains-1 and -2 prevents repair of plasma membrane scrape injuries, but not small pores, and induces a severe muscular dystrophy. Piper AK, Sophocleous RA, Ross SE, Evesson FJ, Saleh O, Bournazos A, Yasa J, Reed C, Woolger N, Sluyter R, Greer P, Biro M, Lemckert FA, Cooper ST. Am J Physiol Cell Physiol. 2020 Jun 1;318(6):C1226-C1237. doi: 10.1152/ajpcell.00408.2019. Epub 2020 Apr 29. PubMed
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. Hegarat N, Crncec A, Suarez Peredo Rodriguez MF, Echegaray Iturra F, Gu Y, Busby O, Lang PF, Barr AR, Bakal C, Kanemaki MT, Lamond AI, Novak B, Ly T, Hochegger H. EMBO J. 2020 Jun 2;39(11):e104419. doi: 10.15252/embj.2020104419. Epub 2020 Apr 30. PubMed
Human pluripotent stem cell-based models suggest preadipocyte senescence as a possible cause of metabolic complications of Werner and Bloom Syndromes. Goh KJ, Chen JH, Rocha N, Semple RK. Sci Rep. 2020 May 4;10(1):7490. doi: 10.1038/s41598-020-64136-8. PubMed
AKT Ser/Thr kinase increases V-ATPase-dependent lysosomal acidification in response to amino acid starvation in mammalian cells. Collins MP, Stransky LA, Forgac M. J Biol Chem. 2020 Jul 10;295(28):9433-9444. doi: 10.1074/jbc.RA120.013223. Epub 2020 May 14. PubMed
HDAC1 inhibition ameliorates TDP-43-induced cell death in vitro and in vivo. Sanna S, Esposito S, Masala A, Sini P, Nieddu G, Galioto M, Fais M, Iaccarino C, Cestra G, Crosio C. Cell Death Dis. 2020 May 14;11(5):369. doi: 10.1038/s41419-020-2580-3. PubMed
Histone H2A variants alpha1-extension helix directs RNF168-mediated ubiquitination. Kelliher JL, West KL, Gong Q, Leung JWC. Nat Commun. 2020 May 18;11(1):2462. doi: 10.1038/s41467-020-16307-4. PubMed
HLTF Promotes Fork Reversal, Limiting Replication Stress Resistance and Preventing Multiple Mechanisms of Unrestrained DNA Synthesis. Bai G, Kermi C, Stoy H, Schiltz CJ, Bacal J, Zaino AM, Hadden MK, Eichman BF, Lopes M, Cimprich KA. Mol Cell. 2020 Jun 18;78(6):1237-1251.e7. doi: 10.1016/j.molcel.2020.04.031. Epub 2020 May 21. PubMed
TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes. Liang H, Zhang P, Bai HJ, Huang J, Yang HT. Stem Cell Res Ther. 2020 May 24;11(1):196. doi: 10.1186/s13287-020-01711-w. PubMed
Role of CD133 in human embryonic stem cell proliferation and teratoma formation. Wang H, Gong P, Li J, Fu Y, Zhou Z, Liu L. Stem Cell Res Ther. 2020 May 27;11(1):208. doi: 10.1186/s13287-020-01729-0. PubMed
Host cell factors stimulate HIV-1 transcription by antagonizing substrate-binding function of Siah1 ubiquitin ligase to stabilize transcription elongation factor ELL2. Wu J, Xue Y, Gao X, Zhou Q. Nucleic Acids Res. 2020 Jul 27;48(13):7321-7332. doi: 10.1093/nar/gkaa461. PubMed
HOXA5 inhibits the proliferation and neoplasia of cervical cancer cells via downregulating the activity of the Wnt/beta-catenin pathway and transactivating TP53. Ma HM, Cui N, Zheng PS. Cell Death Dis. 2020 Jun 4;11(6):420. doi: 10.1038/s41419-020-2629-3. PubMed
Transcriptional consequences of MBD5 disruption in mouse brain and CRISPR-derived neurons. Seabra CM, Aneichyk T, Erdin S, Tai DJC, De Esch CEF, Razaz P, An Y, Manavalan P, Ragavendran A, Stortchevoi A, Abad C, Young JI, Maciel P, Talkowski ME, Gusella JF. Mol Autism. 2020 Jun 5;11(1):45. doi: 10.1186/s13229-020-00354-1. PubMed
Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin-BAF Interaction and BAF Nuclear Localization. Cenni V, Squarzoni S, Loi M, Mattioli E, Lattanzi G, Capanni C. Cells. 2020 Jun 6;9(6). pii: cells9061415. doi: 10.3390/cells9061415. PubMed
TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes. Wetzel L, Blanchard S, Rama S, Beier V, Kaufmann A, Wollert T. Nat Commun. 2020 Jun 12;11(1):2993. doi: 10.1038/s41467-020-16689-5. PubMed
Synergy between SIRT1 and SIRT6 helps recognize DNA breaks and potentiates the DNA damage response and repair in humans and mice. Meng F, Qian M, Peng B, Peng L, Wang X, Zheng K, Liu Z, Tang X, Zhang S, Sun S, Cao X, Pang Q, Zhao B, Ma W, Songyang Z, Xu B, Zhu WG, Xu X, Liu B. Elife. 2020 Jun 15;9. pii: 55828. doi: 10.7554/eLife.55828. PubMed
Sequential CRISPR-Based Screens Identify LITAF and CDIP1 as the Bacillus cereus Hemolysin BL Toxin Host Receptors. Liu J, Zuo Z, Sastalla I, Liu C, Jang JY, Sekine Y, Li Y, Pirooznia M, Leppla SH, Finkel T, Liu S. Cell Host Microbe. 2020 Sep 9;28(3):402-410.e5. doi: 10.1016/j.chom.2020.05.012. Epub 2020 Jun 15. PubMed
Tagging and Deleting of Endogenous Caveolar Components Using CRISPR/Cas9 Technology. Shvets E, Mendoza-Topaz C. Methods Mol Biol. 2020;2169:149-166. doi: 10.1007/978-1-0716-0732-9_14. PubMed
CRISPR-Cas9 generated Pompe knock-in murine model exhibits early-onset hypertrophic cardiomyopathy and skeletal muscle weakness. Huang JY, Kan SH, Sandfeld EK, Dalton ND, Rangel AD, Chan Y, Davis-Turak J, Neumann J, Wang RY. Sci Rep. 2020 Jun 25;10(1):10321. doi: 10.1038/s41598-020-65259-8. PubMed
Loss of function of the mitochondrial peptidase PITRM1 induces proteotoxic stress and Alzheimer's disease-like pathology in human cerebral organoids. Perez MJ, Ivanyuk D, Panagiotakopoulou V, Di Napoli G, Kalb S, Brunetti D, Al-Shaana R, Kaeser SA, Fraschka SA, Jucker M, Zeviani M, Viscomi C, Deleidi M. Mol Psychiatry. 2020 Jul 7. pii: 10.1038/s41380-020-0807-4. doi: 10.1038/s41380-020-0807-4. PubMed
WRNIP1 Is Recruited to DNA Interstrand Crosslinks and Promotes Repair. Socha A, Yang D, Bulsiewicz A, Yaprianto K, Kupculak M, Liang CC, Hadjicharalambous A, Wu R, Gygi SP, Cohn MA. Cell Rep. 2020 Jul 7;32(1):107850. doi: 10.1016/j.celrep.2020.107850. PubMed
WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge. Tang Q, Schaks M, Koundinya N, Yang C, Pollard LW, Svitkina TM, Rottner K, Goode BL. Mol Biol Cell. 2020 Sep 15;31(20):2168-2178. doi: 10.1091/mbc.E19-12-0705. Epub 2020 Jul 22. PubMed
Gravin-associated kinase signaling networks coordinate gamma-tubulin organization at mitotic spindle poles. Bucko PJ, Garcia I, Manocha R, Bhat A, Wordeman L, Scott JD. J Biol Chem. 2020 Oct 2;295(40):13784-13797. doi: 10.1074/jbc.RA120.014791. Epub 2020 Jul 30. PubMed
Cell-substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime. Singh SP, Thomason PA, Lilla S, Schaks M, Tang Q, Goode BL, Machesky LM, Rottner K, Insall RH. PLoS Biol. 2020 Aug 3;18(8):e3000774. doi: 10.1371/journal.pbio.3000774. eCollection 2020 Aug. PubMed
Loss of the Essential Autophagy Regulators FIP200 or Atg5 Leads to Distinct Effects on Focal Adhesion Composition and Organization. Assar EA, Tumbarello DA. Front Cell Dev Biol. 2020 Aug 4;8:733. doi: 10.3389/fcell.2020.00733. eCollection 2020. PubMed

Associated Plasmids

DENR promotes translation reinitiation via ribosome recycling to drive expression of oncogenes including ATF4. Bohlen J, Harbrecht L, Blanco S, Clemm von Hohenberg K, Fenzl K, Kramer G, Bukau B, Teleman AA. Nat Commun. 2020 Sep 16;11(1):4676. doi: 10.1038/s41467-020-18452-2. PubMed
Cell-cycle-gated feedback control mediates desensitization to interferon stimulation. Mudla A, Jiang Y, Arimoto KI, Xu B, Rajesh A, Ryan AP, Wang W, Daugherty MD, Zhang DE, Hao N. Elife. 2020 Sep 18;9. pii: 58825. doi: 10.7554/eLife.58825. PubMed
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