pSpCas9(BB)-2A-Puro (PX459) Citations (514)
Originally described in: Genome engineering using the CRISPR-Cas9 system.Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed Journal
Articles Citing pSpCas9(BB)-2A-Puro (PX459)
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| Intracellular alpha-ketoglutarate maintains the pluripotency of embryonic stem cells. Carey BW, Finley LW, Cross JR, Allis CD, Thompson CB. Nature. 2015 Feb 19;518(7539):413-6. doi: 10.1038/nature13981. Epub 2014 Dec 10. PubMed |
| Generation of genomic deletions in mammalian cell lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J Vis Exp. 2014 Jan 3;(95). doi: 10.3791/52118. PubMed |
| Fatty acid remodeling by LPCAT3 enriches arachidonate in phospholipid membranes and regulates triglyceride transport. Hashidate-Yoshida T, Harayama T, Hishikawa D, Morimoto R, Hamano F, Tokuoka SM, Eto M, Tamura-Nakano M, Yanobu-Takanashi R, Mukumoto Y, Kiyonari H, Okamura T, Kita Y, Shindou H, Shimizu T. Elife. 2015 Apr 21;4. doi: 10.7554/eLife.06328. PubMed |
| A YAP/TAZ-induced feedback mechanism regulates Hippo pathway homeostasis. Moroishi T, Park HW, Qin B, Chen Q, Meng Z, Plouffe SW, Taniguchi K, Yu FX, Karin M, Pan D, Guan KL. Genes Dev. 2015 Jun 15;29(12):1271-84. doi: 10.1101/gad.262816.115. PubMed |
| Xist Exon 7 Contributes to the Stable Localization of Xist RNA on the Inactive X-Chromosome. Yamada N, Hasegawa Y, Yue M, Hamada T, Nakagawa S, Ogawa Y. PLoS Genet. 2015 Aug 5;11(8):e1005430. doi: 10.1371/journal.pgen.1005430. eCollection 2015 Aug. PubMed |
| Alternative Wnt Signaling Activates YAP/TAZ. Park HW, Kim YC, Yu B, Moroishi T, Mo JS, Plouffe SW, Meng Z, Lin KC, Yu FX, Alexander CM, Wang CY, Guan KL. Cell. 2015 Aug 13;162(4):780-94. doi: 10.1016/j.cell.2015.07.013. PubMed |
| CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting. Courtney DG, Moore JE, Atkinson SD, Maurizi E, Allen EH, Pedrioli DM, McLean WH, Nesbit MA, Moore CB. Gene Ther. 2016 Jan;23(1):108-12. doi: 10.1038/gt.2015.82. Epub 2015 Aug 20. PubMed |
Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca(2+) influx.
Jing J, He L, Sun A, Quintana A, Ding Y, Ma G, Tan P, Liang X, Zheng X, Chen L, Shi X, Zhang SL, Zhong L, Huang Y, Dong MQ, Walker CL, Hogan PG, Wang Y, Zhou Y.
Nat Cell Biol. 2015 Oct;17(10):1339-47. doi: 10.1038/ncb3234. Epub 2015 Aug 31.
PubMed
Associated Plasmids |
| Nanopatterned Human iPSC-based Model of a Dystrophin-Null Cardiomyopathic Phenotype. Macadangdang J, Guan X, Smith AS, Lucero R, Czerniecki S, Childers MK, Mack DL, Kim DH. Cell Mol Bioeng. 2015 Sep;8(3):320-332. PubMed |
| Feedback Mechanisms Regulate Ets Variant 2 (Etv2) Gene Expression and Hematoendothelial Lineages. Koyano-Nakagawa N, Shi X, Rasmussen TL, Das S, Walter CA, Garry DJ. J Biol Chem. 2015 Nov 20;290(47):28107-19. doi: 10.1074/jbc.M115.662197. Epub 2015 Sep 22. PubMed |
| Inhibition of beta-catenin-TCF1 interaction delays differentiation of mouse embryonic stem cells. Chatterjee SS, Saj A, Gocha T, Murphy M, Gonsalves FC, Zhang X, Hayward P, Akgol Oksuz B, Shen SS, Madar A, Martinez Arias A, DasGupta R. J Cell Biol. 2015 Oct 12;211(1):39-51. doi: 10.1083/jcb.201503017. PubMed |
| CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in neurodevelopment. Wang P, Lin M, Pedrosa E, Hrabovsky A, Zhang Z, Guo W, Lachman HM, Zheng D. Mol Autism. 2015 Oct 19;6:55. doi: 10.1186/s13229-015-0048-6. eCollection 2015. PubMed |
| CTCF Binding Polarity Determines Chromatin Looping. de Wit E, Vos ES, Holwerda SJ, Valdes-Quezada C, Verstegen MJ, Teunissen H, Splinter E, Wijchers PJ, Krijger PH, de Laat W. Mol Cell. 2015 Nov 19;60(4):676-84. doi: 10.1016/j.molcel.2015.09.023. Epub 2015 Oct 29. PubMed |
| Blocking the association of HDAC4 with MAP1S accelerates autophagy clearance of mutant Huntingtin. Yue F, Li W, Zou J, Chen Q, Xu G, Huang H, Xu Z, Zhang S, Gallinari P, Wang F, McKeehan WL, Liu L. Aging (Albany NY). 2015 Oct;7(10):839-53. PubMed |
| The primate-specific noncoding RNA HPAT5 regulates pluripotency during human preimplantation development and nuclear reprogramming. Durruthy-Durruthy J, Sebastiano V, Wossidlo M, Cepeda D, Cui J, Grow EJ, Davila J, Mall M, Wong WH, Wysocka J, Au KF, Reijo Pera RA. Nat Genet. 2016 Jan;48(1):44-52. doi: 10.1038/ng.3449. Epub 2015 Nov 23. PubMed |
| The Hippo pathway effectors YAP and TAZ promote cell growth by modulating amino acid signaling to mTORC1. Hansen CG, Ng YL, Lam WL, Plouffe SW, Guan KL. Cell Res. 2015 Dec;25(12):1299-313. doi: 10.1038/cr.2015.140. Epub 2015 Nov 27. PubMed |
| FGF2 mediates hepatic progenitor cell formation during human pluripotent stem cell differentiation by inducing the WNT antagonist NKD1. Twaroski K, Mallanna SK, Jing R, DiFurio F, Urick A, Duncan SA. Genes Dev. 2015 Dec 1;29(23):2463-74. doi: 10.1101/gad.268961.115. PubMed |
| Reduced cholesterol levels impair Smoothened activation in Smith-Lemli-Opitz syndrome. Blassberg R, Macrae JI, Briscoe J, Jacob J. Hum Mol Genet. 2015 Dec 18. pii: ddv507. PubMed |
| Aurora A phosphorylation of WD40-repeat protein 62 in mitotic spindle regulation. Lim NR, Yeap YY, Ang CS, Williamson NA, Bogoyevitch MA, Quinn LM, Ng DC. Cell Cycle. 2016;15(3):413-24. doi: 10.1080/15384101.2015.1127472. PubMed |
| D-repeat in the XIST gene is required for X chromosome inactivation. Lv Q, Yuan L, Song Y, Sui T, Li Z, Lai L. RNA Biol. 2016 Jan 19:0. PubMed |
| Lack of MG53 in human heart precludes utility as a biomarker of myocardial injury or endogenous cardioprotective factor. Lemckert FA, Bournazos A, Eckert DM, Kenzler M, Hawkes JM, Butler TL, Ceely B, North KN, Winlaw DS, Egan JR, Cooper ST. Cardiovasc Res. 2016 Jan 19. pii: cvw017. PubMed |
| A Minor Subset of Super Elongation Complexes Plays a Predominant Role in Reversing HIV-1 Latency. Li Z, Lu H, Zhou Q. Mol Cell Biol. 2016 Feb 1;36(7):1194-205. doi: 10.1128/MCB.00994-15. PubMed |
| The Tyrosine Kinase Pyk2 Contributes to Complement-Mediated Phagocytosis in Murine Macrophages. Paone C, Rodrigues N, Ittner E, Santos C, Buntru A, Hauck CR. J Innate Immun. 2016 Feb 6. PubMed |
| Tandem repeat knockout utilizing the CRISPR/Cas9 system in human cells. Lv Q, Lai L, Yuan L, Song Y, Sui T, Li Z. Gene. 2016 May 15;582(2):122-7. doi: 10.1016/j.gene.2016.02.013. Epub 2016 Feb 9. PubMed |
| Human GBP1 does not localise to pathogen vacuoles but restricts Toxoplasma gondii. Johnston AC, Piro A, Clough B, Siew M, Virreira Winter S, Coers J, Frickel EM. Cell Microbiol. 2016 Feb 13. doi: 10.1111/cmi.12579. PubMed |
| Dynamics of CDKN1A in Single Cells Defined by an Endogenous Fluorescent Tagging Toolkit. Stewart-Ornstein J, Lahav G. Cell Rep. 2016 Feb 10. pii: S2211-1247(16)30023-7. doi: 10.1016/j.celrep.2016.01.045. PubMed |
| Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases. Yang D, Scavuzzo MA, Chmielowiec J, Sharp R, Bajic A, Borowiak M. Sci Rep. 2016 Feb 18;6:21264. doi: 10.1038/srep21264. PubMed |
| CHO cells knocked out for TSC2 display an improved productivity of antibodies under fed batch conditions. McVey D, Aronov M, Rizzi G, Cowan A, Scott C, Megill J, Russell R, Tirosh B. Biotechnol Bioeng. 2016 Feb 16. doi: 10.1002/bit.25951. PubMed |
| RNA-binding Protein PCBP2 Regulates p73 Expression and p73-dependent Antioxidant Defense. Ren C, Zhang J, Yan W, Zhang Y, Chen X. J Biol Chem. 2016 Apr 29;291(18):9629-37. doi: 10.1074/jbc.M115.712125. Epub 2016 Feb 23. PubMed |
| A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation. Li X, Rydzewski N, Hider A, Zhang X, Yang J, Wang W, Gao Q, Cheng X, Xu H. Nat Cell Biol. 2016 Apr;18(4):404-17. doi: 10.1038/ncb3324. Epub 2016 Mar 7. PubMed |
| Overcoming doxorubicin resistance of cancer cells by Cas9-mediated gene disruption. Ha JS, Byun J, Ahn DR. Sci Rep. 2016 Mar 10;6:22847. doi: 10.1038/srep22847. PubMed |
| AMPK Activation and Metabolic Reprogramming by Tamoxifen through Estrogen Receptor-Independent Mechanisms Suggests New Uses for This Therapeutic Modality in Cancer Treatment. Daurio NA, Tuttle SW, Worth AJ, Song EY, Davis JM, Snyder NW, Blair IA, Koumenis C. Cancer Res. 2016 Jun 1;76(11):3295-306. doi: 10.1158/0008-5472.CAN-15-2197. Epub 2016 Mar 28. PubMed |
| Development of Novel Small Hairpin RNAs That do not Require Processing by Dicer or AGO2. Ohno S, Itano K, Harada Y, Asada K, Oikawa K, Kashiwazako M, Okuyama H, Kumagai K, Takanashi M, Sudo K, Ikeda N, Kuroda M. Mol Ther. 2016 Aug;24(7):1278-89. doi: 10.1038/mt.2016.81. Epub 2016 Apr 25. PubMed |
| Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network. Crevenna AH, Blank B, Maiser A, Emin D, Prescher J, Beck G, Kienzle C, Bartnik K, Habermann B, Pakdel M, Leonhardt H, Lamb DC, von Blume J. J Cell Biol. 2016 May 9;213(3):305-14. doi: 10.1083/jcb.201601089. Epub 2016 May 2. PubMed |
| Efficient generation of FVII gene knockout mice using CRISPR/Cas9 nuclease and truncated guided RNAs. An L, Hu Y, Chang S, Zhu X, Ling P, Zhang F, Liu J, Liu Y, Chen Y, Yang L, Presicce GA, Du F. Sci Rep. 2016 May 3;6:25199. doi: 10.1038/srep25199. PubMed |
| AMPK Causes Cell Cycle Arrest in LKB1-deficient Cells via Activation of CAMKK2. Fogarty S, Ross FA, Vara Ciruelos D, Gray A, Gowans GJ, Hardie DG. Mol Cancer Res. 2016 May 2. pii: molcanres.0479.2015. PubMed |
| The antitumor toxin CD437 is a direct inhibitor of DNA polymerase alpha. Han T, Goralski M, Capota E, Padrick SB, Kim J, Xie Y, Nijhawan D. Nat Chem Biol. 2016 Jul;12(7):511-5. doi: 10.1038/nchembio.2082. Epub 2016 May 16. PubMed |
| Golgb1 regulates protein glycosylation and is crucial for mammalian palate development. Lan Y, Zhang N, Liu H, Xu J, Jiang R. Development. 2016 Jul 1;143(13):2344-55. doi: 10.1242/dev.134577. Epub 2016 May 25. PubMed |
| Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/Cas9-Mediated Gene Editing. Bierle CJ, Anderholm KM, Wang JB, McVoy MA, Schleiss MR. J Virol. 2016 Jul 11;90(15):6989-98. doi: 10.1128/JVI.00139-16. Print 2016 Aug 1. PubMed |
| Keratin 8-deletion induced colitis predisposes to murine colorectal cancer enforced by the inflammasome and IL-22 pathway. Misiorek JO, Lahdeniemi IA, Nystrom JH, Paramonov VM, Gullmets JA, Saarento H, Rivero-Muller A, Husoy T, Taimen P, Toivola DM. Carcinogenesis. 2016 May 27. pii: bgw063. PubMed |
| Immuno-targeting the multifunctional CD38 using nanobody. Li T, Qi S, Unger M, Hou YN, Deng QW, Liu J, Lam CM, Wang XW, Xin D, Zhang P, Koch-Nolte F, Hao Q, Zhang H, Lee HC, Zhao YJ. Sci Rep. 2016 Jun 2;6:27055. doi: 10.1038/srep27055. PubMed |
| A conserved phosphatase destroys toxic glycolytic side products in mammals and yeast. Collard F, Baldin F, Gerin I, Bolsee J, Noel G, Graff J, Veiga-da-Cunha M, Stroobant V, Vertommen D, Houddane A, Rider MH, Linster CL, Van Schaftingen E, Bommer GT. Nat Chem Biol. 2016 Aug;12(8):601-7. doi: 10.1038/nchembio.2104. Epub 2016 Jun 13. PubMed |
| Predominant Expression of Hybrid N-Glycans Has Distinct Cellular Roles Relative to Complex and Oligomannose N-Glycans. Hall MK, Weidner DA, Zhu Y, Dayal S, Whitman AA, Schwalbe RA. Int J Mol Sci. 2016 Jun 13;17(6). pii: E925. doi: 10.3390/ijms17060925. PubMed |
| The ubiquitin-like protein FAT10 stabilizes eEF1A1 expression to promote tumor proliferation in a complex manner. Liu X, Chen L, Ge J, Yan C, Huang Z, Hu J, Wen C, Li M, Huang D, Qiu Y, Hao H, Yuan R, Lei J, Yu X, Shao J. Cancer Res. 2016 Jun 16. pii: canres.3118.2015. PubMed |
| Fatostatin blocks ER exit of SCAP but inhibits cell growth in a SCAP-independent manner. Shao W, Machamer CE, Espenshade PJ. J Lipid Res. 2016 Jun 20. pii: jlr.M069583. PubMed |
| Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H196. Marthaler AG, Schmid B, Tubsuwan A, Poulsen UB, Engelbrecht AF, Mau-Holzmann UA, Hyttel P, Nielsen JE, Nielsen TT, Holst B. Stem Cell Res. 2016 Jan;16(1):162-5. doi: 10.1016/j.scr.2015.12.031. Epub 2016 Jan 3. PubMed |
| Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H266. Marthaler AG, Tubsuwan A, Schmid B, Poulsen UB, Engelbrecht AF, Mau-Holzmann UA, Hyttel P, Nielsen TT, Nielsen JE, Holst B. Stem Cell Res. 2016 Jan;16(1):202-5. doi: 10.1016/j.scr.2015.12.048. Epub 2016 Jan 15. PubMed |
| Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase. Baumann S, Hennet T. J Biol Chem. 2016 Aug 26;291(35):18514-24. doi: 10.1074/jbc.M116.723379. Epub 2016 Jul 11. PubMed |
| A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. Lambrus BG, Daggubati V, Uetake Y, Scott PM, Clutario KM, Sluder G, Holland AJ. J Cell Biol. 2016 Jul 18;214(2):143-53. doi: 10.1083/jcb.201604054. PubMed |
| 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. Meitinger F, Anzola JV, Kaulich M, Richardson A, Stender JD, Benner C, Glass CK, Dowdy SF, Desai A, Shiau AK, Oegema K. J Cell Biol. 2016 Jul 18;214(2):155-66. doi: 10.1083/jcb.201604081. PubMed |
| Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. Kubota Y, Nomura K, Katoh Y, Yamashita R, Kaneko K, Furuyama K. J Biol Chem. 2016 Sep 23;291(39):20516-29. doi: 10.1074/jbc.M116.719161. Epub 2016 Aug 5. PubMed |
| Loss-of-function mutations in the RNA biogenesis factor NAF1 predispose to pulmonary fibrosis-emphysema. Stanley SE, Gable DL, Wagner CL, Carlile TM, Hanumanthu VS, Podlevsky JD, Khalil SE, DeZern AE, Rojas-Duran MF, Applegate CD, Alder JK, Parry EM, Gilbert WV, Armanios M. Sci Transl Med. 2016 Aug 10;8(351):351ra107. doi: 10.1126/scitranslmed.aaf7837. PubMed |
| CRISPR/Cas9-Mediated Trp53 and Brca2 Knockout to Generate Improved Murine Models of Ovarian High-Grade Serous Carcinoma. Walton J, Blagih J, Ennis D, Leung E, Dowson S, Farquharson M, Tookman LA, Orange C, Athineos D, Mason S, Stevenson D, Blyth K, Strathdee D, Balkwill FR, Vousden K, Lockley M, McNeish IA. Cancer Res. 2016 Oct 15;76(20):6118-6129. Epub 2016 Aug 16. PubMed |
| CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice. Oji A, Noda T, Fujihara Y, Miyata H, Kim YJ, Muto M, Nozawa K, Matsumura T, Isotani A, Ikawa M. Sci Rep. 2016 Aug 17;6:31666. doi: 10.1038/srep31666. PubMed |
| The Non-coding Mammary Carcinoma Susceptibility Locus, Mcs5c, Regulates Pappa Expression via Age-Specific Chromatin Folding and Allele-Dependent DNA Methylation. Henning AN, Haag JD, Smits BM, Gould MN. PLoS Genet. 2016 Aug 18;12(8):e1006261. doi: 10.1371/journal.pgen.1006261. eCollection 2016 Aug. PubMed |
| An endosomal tether undergoes an entropic collapse to bring vesicles together. Murray DH, Jahnel M, Lauer J, Avellaneda MJ, Brouilly N, Cezanne A, Morales-Navarrete H, Perini ED, Ferguson C, Lupas AN, Kalaidzidis Y, Parton RG, Grill SW, Zerial M. Nature. 2016 Sep 1;537(7618):107-111. doi: 10.1038/nature19326. Epub 2016 Aug 24. PubMed |
| Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex. Zhang X, Chen MH, Wu X, Kodani A, Fan J, Doan R, Ozawa M, Ma J, Yoshida N, Reiter JF, Black DL, Kharchenko PV, Sharp PA, Walsh CA. Cell. 2016 Aug 25;166(5):1147-1162.e15. doi: 10.1016/j.cell.2016.07.025. PubMed |
| An Effective Molecular Target Site in Hepatitis B Virus S Gene for Cas9 Cleavage and Mutational Inactivation. Li H, Sheng C, Liu H, Liu G, Du X, Du J, Zhan L, Li P, Yang C, Qi L, Wang J, Yang X, Jia L, Xie J, Wang L, Hao R, Xu D, Tong Y, Zhou Y, Zhou J, Sun Y, Li Q, Qiu S, Song H. Int J Biol Sci. 2016 Aug 5;12(9):1104-13. doi: 10.7150/ijbs.16064. eCollection 2016. PubMed |
| Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Makino A, Hullin-Matsuda F, Murate M, Abe M, Tomishige N, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton I, Kobayashi T. Mol Biol Cell. 2016 Nov 1;27(21):3293-3304. Epub 2016 Aug 31. PubMed |
| CRISPR-Cas9 directed knock-out of a constitutively expressed gene using lance array nanoinjection. Sessions JW, Skousen CS, Price KD, Hanks BW, Hope S, Alder JK, Jensen BD. Springerplus. 2016 Sep 9;5(1):1521. doi: 10.1186/s40064-016-3037-0. eCollection 2016. PubMed |
| Quantitative Lipid Droplet Proteome Analysis Identifies Annexin A3 as a Cofactor for HCV Particle Production. Rosch K, Kwiatkowski M, Hofmann S, Schobel A, Gruttner C, Wurlitzer M, Schluter H, Herker E. Cell Rep. 2016 Sep 20;16(12):3219-31. doi: 10.1016/j.celrep.2016.08.052. PubMed |
| The Circadian Rhythm Gene Arntl2 Is a Metastasis Susceptibility Gene for Estrogen Receptor-Negative Breast Cancer. Ha NH, Long J, Cai Q, Shu XO, Hunter KW. PLoS Genet. 2016 Sep 22;12(9):e1006267. doi: 10.1371/journal.pgen.1006267. eCollection 2016 Sep. PubMed |
| RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes. Rose NR, King HW, Blackledge NP, Fursova NA, Ember KJ, Fischer R, Kessler BM, Klose RJ. Elife. 2016 Oct 5;5. pii: e18591. doi: 10.7554/eLife.18591. PubMed |
| ETAA1 acts at stalled replication forks to maintain genome integrity. Bass TE, Luzwick JW, Kavanaugh G, Carroll C, Dungrawala H, Glick GG, Feldkamp MD, Putney R, Chazin WJ, Cortez D. Nat Cell Biol. 2016 Nov;18(11):1185-1195. doi: 10.1038/ncb3415. Epub 2016 Oct 10. PubMed |
| The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane. Tanaka Y, Ono N, Shima T, Tanaka G, Katoh Y, Nakayama K, Takatsu H, Shin HW. Mol Biol Cell. 2016 Dec 1;27(24):3883-3893. Epub 2016 Oct 12. PubMed |
| Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis. Sato K, Kimura M, Sugiyama K, Nishikawa M, Okano Y, Nagaoka H, Nagase T, Kitade Y, Ueda H. J Biol Chem. 2016 Nov 25;291(48):25227-25238. Epub 2016 Oct 20. PubMed |
| Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions. Martins R, Maier J, Gorki AD, Huber KV, Sharif O, Starkl P, Saluzzo S, Quattrone F, Gawish R, Lakovits K, Aichinger MC, Radic-Sarikas B, Lardeau CH, Hladik A, Korosec A, Brown M, Vaahtomeri K, Duggan M, Kerjaschki D, Esterbauer H, Colinge J, Eisenbarth SC, Decker T, Bennett KL, Kubicek S, Sixt M, Superti-Furga G, Knapp S. Nat Immunol. 2016 Dec;17(12):1361-1372. doi: 10.1038/ni.3590. Epub 2016 Oct 31. PubMed |
| RHO binding to FAM65A regulates Golgi reorientation during cell migration. Mardakheh FK, Self A, Marshall CJ. J Cell Sci. 2016 Dec 15;129(24):4466-4479. Epub 2016 Nov 2. PubMed |
| Inactivation of the type I interferon pathway reveals long double-stranded RNA-mediated RNA interference in mammalian cells. Maillard PV, Van der Veen AG, Deddouche-Grass S, Rogers NC, Merits A, Reis E Sousa C. EMBO J. 2016 Dec 1;35(23):2505-2518. Epub 2016 Nov 4. PubMed |
| CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening. Koferle A, Worf K, Breunig C, Baumann V, Herrero J, Wiesbeck M, Hutter LH, Gotz M, Fuchs C, Beck S, Stricker SH. BMC Genomics. 2016 Nov 14;17(1):917. PubMed |
| Generation of a gene-corrected isogenic control cell line from an Alzheimer's disease patient iPSC line carrying a A79V mutation in PSEN1. Pires C, Schmid B, Petraeus C, Poon A, Nimsanor N, Nielsen TT, Waldemar G, Hjermind LE, Nielsen JE, Hyttel P, Freude KK. Stem Cell Res. 2016 Sep;17(2):285-288. doi: 10.1016/j.scr.2016.08.002. Epub 2016 Aug 7. PubMed |
| Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9. Herring A, Messana A, Bara AM, Hazelbaker DZ, Eggan K, Barrett LE. Stem Cell Res. 2016 Sep;17(2):430-432. doi: 10.1016/j.scr.2016.09.009. Epub 2016 Sep 16. PubMed |
| Ubiquitylation of Ku80 by RNF126 promotes completion of NHEJ-mediated DNA repair. Ishida N, Nakagawa T, Iemura SI, Yasui A, Shima H, Katoh Y, Nagasawa Y, Natsume T, Igarashi K, Nakayama K. Mol Cell Biol. 2016 Nov 28. pii: MCB.00347-16. PubMed |
| The Hippo Pathway Kinases LATS1/2 Suppress Cancer Immunity. Moroishi T, Hayashi T, Pan WW, Fujita Y, Holt MV, Qin J, Carson DA, Guan KL. Cell. 2016 Dec 1;167(6):1525-1539.e17. doi: 10.1016/j.cell.2016.11.005. PubMed |
| Characterization of Hippo Pathway Components by Gene Inactivation. Plouffe SW, Meng Z, Lin KC, Lin B, Hong AW, Chun JV, Guan KL. Mol Cell. 2016 Dec 1;64(5):993-1008. doi: 10.1016/j.molcel.2016.10.034. PubMed |
| Regulation of ciliary retrograde protein trafficking by Joubert syndrome proteins ARL13B and INPP5E. Nozaki S, Katoh Y, Terada M, Michisaka S, Funabashi T, Takahashi S, Kontani K, Nakayama K. J Cell Sci. 2016 Dec 7. pii: jcs.197004. PubMed |
| Intraflagellar transport-A complex mediates ciliary entry as well as retrograde trafficking of ciliary G protein-coupled receptors. Hirano T, Katoh Y, Nakayama K. Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-11-0813. PubMed |
| CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma. Phelps MP, Bailey JN, Vleeshouwer-Neumann T, Chen EY. Proc Natl Acad Sci U S A. 2016 Dec 27;113(52):15090-15095. doi: 10.1073/pnas.1610270114. Epub 2016 Dec 12. PubMed |
| Delta40p53 suppresses tumor cell proliferation and induces cellular senescence in hepatocellular carcinoma cells. Ota A, Nakao H, Sawada Y, Karnan S, Wahiduzzaman M, Inoue T, Kobayashi Y, Yamamoto T, Ishii N, Ohashi T, Nakade Y, Sato K, Itoh K, Konishi H, Hosokawa Y, Yoneda M. J Cell Sci. 2016 Dec 15. pii: jcs.190736. PubMed |
| Random Splicing of Several Exons Caused by a Single Base Change in the Target Exon of CRISPR/Cas9 Mediated Gene Knockout. Kapahnke M, Banning A, Tikkanen R. Cells. 2016 Dec 14;5(4). pii: E45. PubMed |
| Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Makino S, Fukumura R, Gondo Y. Sci Rep. 2016 Dec 21;6:39608. doi: 10.1038/srep39608. PubMed |
| Deletion of the GAA repeats from the human frataxin gene using the CRISPR-Cas9 system in YG8R-derived cells and mouse models of Friedreich ataxia. Ouellet DL, Cherif K, Rousseau J, Tremblay JP. Gene Ther. 2017 May;24(5):265-274. doi: 10.1038/gt.2016.89. Epub 2016 Dec 26. PubMed |
| TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP). Li MM, Lau Z, Cheung P, Aguilar EG, Schneider WM, Bozzacco L, Molina H, Buehler E, Takaoka A, Rice CM, Felsenfeld DP, MacDonald MR. PLoS Pathog. 2017 Jan 6;13(1):e1006145. doi: 10.1371/journal.ppat.1006145. PubMed |
| A Fluorophore Fusion-construct of Human Profilin I with Non-compromized poly(L-Proline) Binding capacity suitable for Imaging. Nejedla M, Li Z, Masser AE, Biancospino M, Spiess M, Mackowiak SD, Friedlander MR, Karlsson R. J Mol Biol. 2017 Jan 7. pii: S0022-2836(17)30018-9. doi: 10.1016/j.jmb.2017.01.004. PubMed |
| Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Funabashi T, Katoh Y, Michisaka S, Terada M, Sugawa M, Nakayama K. Mol Biol Cell. 2017 Mar 1;28(5):624-633. doi: 10.1091/mbc.E16-09-0648. Epub 2017 Jan 11. PubMed |
| Structural basis for ELL2 and AFF4 activation of HIV-1 proviral transcription. Qi S, Li Z, Schulze-Gahmen U, Stjepanovic G, Zhou Q, Hurley JH. Nat Commun. 2017 Jan 30;8:14076. doi: 10.1038/ncomms14076. PubMed |
| Filopodia formation and endosome clustering induced by mutant plus-end-directed myosin VI. Masters TA, Buss F. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1595-1600. doi: 10.1073/pnas.1616941114. Epub 2017 Jan 31. PubMed |
Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system.
Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, Nakayama K.
Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051.
PubMed
Associated Plasmids |
| MARK4 inhibits Hippo signaling to promote proliferation and migration of breast cancer cells. Heidary Arash E, Shiban A, Song S, Attisano L. EMBO Rep. 2017 Mar;18(3):420-436. doi: 10.15252/embr.201642455. Epub 2017 Feb 9. PubMed |
| ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Leung JW, Makharashvili N, Agarwal P, Chiu LY, Pourpre R, Cammarata MB, Cannon JR, Sherker A, Durocher D, Brodbelt JS, Paull TT, Miller KM. Genes Dev. 2017 Feb 1;31(3):260-274. doi: 10.1101/gad.292516.116. Epub 2017 Feb 27. PubMed |
| MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells. Ito K, Eguchi Y, Imagawa Y, Akai S, Mochizuki H, Tsujimoto Y. Cell Death Discov. 2017 Feb 27;3:17013. doi: 10.1038/cddiscovery.2017.13. eCollection 2017. PubMed |
Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells.
Lin L, Petersen TS, Jensen KT, Bolund L, Kuhn R, Luo Y.
J Biotechnol. 2017 Mar 1;247:42-49. doi: 10.1016/j.jbiotec.2017.02.024.
PubMed
Associated Plasmids |
| CRISPR system for genome engineering: the application for autophagy study. Cui J, Chew SJL, Shi Y, Gong Z, Shen HM. BMB Rep. 2017 May;50(5):247-256. PubMed |
| Re-evaluating the roles of myosin 18Aalpha and F-actin in determining Golgi morphology. Bruun K, Beach JR, Heissler SM, Remmert K, Sellers JR, Hammer JA. Cytoskeleton (Hoboken). 2017 May;74(5):205-218. doi: 10.1002/cm.21364. Epub 2017 May 10. PubMed |
| Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus. De Jaime-Soguero A, Aulicino F, Ertaylan G, Griego A, Cerrato A, Tallam A, Del Sol A, Cosma MP, Lluis F. PLoS Genet. 2017 Mar 27;13(3):e1006682. doi: 10.1371/journal.pgen.1006682. eCollection 2017 Mar. PubMed |
| MED12-related XLID disorders are dose-dependent of immediate early genes (IEGs) expression. Donnio LM, Bidon B, Hashimoto S, May M, Epanchintsev A, Ryan C, Allen W, Hackett A, Gecz J, Skinner C, Stevenson RE, de Brouwer APM, Coutton C, Francannet C, Jouk PS, Schwartz CE, Egly JM. Hum Mol Genet. 2017 Jun 1;26(11):2062-2075. doi: 10.1093/hmg/ddx099. PubMed |
| A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3'end using CRISPR-Cas9 genome editing technique. Basu S, Adams L, Guhathakurta S, Kim YS. Sci Rep. 2017 Apr 4;8:45883. doi: 10.1038/srep45883. PubMed |
| FKBP8 recruits LC3A to mediate Parkin-independent mitophagy. Bhujabal Z, Birgisdottir AB, Sjottem E, Brenne HB, Overvatn A, Habisov S, Kirkin V, Lamark T, Johansen T. EMBO Rep. 2017 Jun;18(6):947-961. doi: 10.15252/embr.201643147. Epub 2017 Apr 5. PubMed |
| Removal of Integrated Hepatitis B Virus DNA Using CRISPR-Cas9. Li H, Sheng C, Wang S, Yang L, Liang Y, Huang Y, Liu H, Li P, Yang C, Yang X, Jia L, Xie J, Wang L, Hao R, Du X, Xu D, Zhou J, Li M, Sun Y, Tong Y, Li Q, Qiu S, Song H. Front Cell Infect Microbiol. 2017 Mar 22;7:91. doi: 10.3389/fcimb.2017.00091. eCollection 2017. PubMed |
| MIEF1/2 function as adaptors to recruit Drp1 to mitochondria and regulate the association of Drp1 with Mff. Yu R, Liu T, Jin SB, Ning C, Lendahl U, Nister M, Zhao J. Sci Rep. 2017 Apr 13;7(1):880. doi: 10.1038/s41598-017-00853-x. PubMed |
| RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly. Nishijima Y, Hagiya Y, Kubo T, Takei R, Katoh Y, Nakayama K. Mol Biol Cell. 2017 Jun 15;28(12):1652-1666. doi: 10.1091/mbc.E17-01-0017. Epub 2017 Apr 20. PubMed |
| A Gene Regulatory Network Balances Neural and Mesoderm Specification during Vertebrate Trunk Development. Gouti M, Delile J, Stamataki D, Wymeersch FJ, Huang Y, Kleinjung J, Wilson V, Briscoe J. Dev Cell. 2017 May 8;41(3):243-261.e7. doi: 10.1016/j.devcel.2017.04.002. Epub 2017 Apr 27. PubMed |
| Glucocorticoid Receptor:MegaTrans Switching Mediates the Repression of an ERalpha-Regulated Transcriptional Program. Yang F, Ma Q, Liu Z, Li W, Tan Y, Jin C, Ma W, Hu Y, Shen J, Ohgi KA, Telese F, Liu W, Rosenfeld MG. Mol Cell. 2017 May 4;66(3):321-331.e6. doi: 10.1016/j.molcel.2017.03.019. PubMed |
| Golga5 is dispensable for mouse embryonic development and postnatal survival. McGee LJ, Jiang AL, Lan Y. Genesis. 2017 Jul;55(7). doi: 10.1002/dvg.23039. Epub 2017 Jun 2. PubMed |
| The New Chemical Reporter 6-Alkynyl-6-deoxy-GlcNAc Reveals O-GlcNAc Modification of the Apoptotic Caspases That Can Block the Cleavage/Activation of Caspase-8. Chuh KN, Batt AR, Zaro BW, Darabedian N, Marotta NP, Brennan CK, Amirhekmat A, Pratt MR. J Am Chem Soc. 2017 Jun 14;139(23):7872-7885. doi: 10.1021/jacs.7b02213. Epub 2017 May 31. PubMed |
| Activation of Adiponectin Receptor Regulates Proprotein Convertase Subtilisin/Kexin Type 9 Expression and Inhibits Lesions in ApoE-Deficient Mice. Sun L, Yang X, Li Q, Zeng P, Liu Y, Liu L, Chen Y, Yu M, Ma C, Li X, Li Y, Zhang R, Zhu Y, Miao QR, Han J, Duan Y. Arterioscler Thromb Vasc Biol. 2017 Jul;37(7):1290-1300. doi: 10.1161/ATVBAHA.117.309630. Epub 2017 May 25. PubMed |
| Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency. Jensen KT, Floe L, Petersen TS, Huang J, Xu F, Bolund L, Luo Y, Lin L. FEBS Lett. 2017 Jul;591(13):1892-1901. doi: 10.1002/1873-3468.12707. Epub 2017 Jun 28. PubMed |
| Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2. Tchasovnikarova IA, Timms RT, Douse CH, Roberts RC, Dougan G, Kingston RE, Modis Y, Lehner PJ. Nat Genet. 2017 Jul;49(7):1035-1044. doi: 10.1038/ng.3878. Epub 2017 Jun 5. PubMed |
| Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time. White CW, Vanyai HK, See HB, Johnstone EKM, Pfleger KDG. Sci Rep. 2017 Jun 9;7(1):3187. doi: 10.1038/s41598-017-03486-2. PubMed |
| Viral Replication Complexes Are Targeted by LC3-Guided Interferon-Inducible GTPases. Biering SB, Choi J, Halstrom RA, Brown HM, Beatty WL, Lee S, McCune BT, Dominici E, Williams LE, Orchard RC, Wilen CB, Yamamoto M, Coers J, Taylor GA, Hwang S. Cell Host Microbe. 2017 Jul 12;22(1):74-85.e7. doi: 10.1016/j.chom.2017.06.005. Epub 2017 Jun 29. PubMed |
| Syk Plays a Critical Role in the Expression and Activation of IRAK1 in LPS-Treated Macrophages. Park JG, Son YJ, Yoo BC, Yang WS, Kim JH, Kim JH, Cho JY. Mediators Inflamm. 2017;2017:1506248. doi: 10.1155/2017/1506248. Epub 2017 Jun 7. PubMed |
| Regulatory Expansion in Mammals of Multivalent hnRNP Assemblies that Globally Control Alternative Splicing. Gueroussov S, Weatheritt RJ, O'Hanlon D, Lin ZY, Narula A, Gingras AC, Blencowe BJ. Cell. 2017 Jul 13;170(2):324-339.e23. doi: 10.1016/j.cell.2017.06.037. PubMed |
| Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer. Nabet BY, Qiu Y, Shabason JE, Wu TJ, Yoon T, Kim BC, Benci JL, DeMichele AM, Tchou J, Marcotrigiano J, Minn AJ. Cell. 2017 Jul 13;170(2):352-366.e13. doi: 10.1016/j.cell.2017.06.031. PubMed |
Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.
Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, Lin SC.
Nature. 2017 Aug 3;548(7665):112-116. doi: 10.1038/nature23275. Epub 2017 Jul 19.
PubMed
Associated Plasmids |
| A time-resolved molecular map of the macrophage response to VSV infection. Kandasamy RK, Vladimer GI, Snijder B, Muller AC, Rebsamen M, Bigenzahn JW, Moskovskich A, Sabler M, Stefanovic A, Scorzoni S, Bruckner M, Penz T, Cleary C, Kralovics R, Colinge J, Bennett KL, Superti-Furga G. NPJ Syst Biol Appl. 2016 Oct 27;2:16027. doi: 10.1038/npjsba.2016.27. eCollection 2016. PubMed |
| Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families. Voloshanenko O, Gmach P, Winter J, Kranz D, Boutros M. FASEB J. 2017 Nov;31(11):4832-4844. doi: 10.1096/fj.201700144R. Epub 2017 Jul 21. PubMed |
| The Axis of CXCR4/SDF-1 Plays a Role in Colon Cancer Cell Adhesion Through Regulation of the AKT and IGF1R Signalling Pathways. Zheng F, Zhang Z, Flamini V, Jiang WG, Cui Y. Anticancer Res. 2017 Aug;37(8):4361-4369. doi: 10.21873/anticanres.11830. PubMed |
| Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration. Banks CJ, Rodriguez NW, Gashler KR, Pandya RR, Mortenson JB, Whited MD, Soderblom EJ, Thompson JW, Moseley MA, Reddi AR, Tessem JS, Torres MP, Bikman BT, Andersen JL. Mol Cell Biol. 2017 Sep 26;37(20). pii: MCB.00354-17. doi: 10.1128/MCB.00354-17. Print 2017 Oct 15. PubMed |
| Regulation of Hippo pathway transcription factor TEAD by p38 MAPK-induced cytoplasmic translocation. Lin KC, Moroishi T, Meng Z, Jeong HS, Plouffe SW, Sekido Y, Han J, Park HW, Guan KL. Nat Cell Biol. 2017 Jul 28;19(8):996-1002. doi: 10.1038/ncb3581. PubMed |
| Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein. Scott CA, Marsden AN, Rebagliati MR, Zhang Q, Chamling X, Searby CC, Baye LM, Sheffield VC, Slusarski DC. PLoS Genet. 2017 Jul 28;13(7):e1006936. doi: 10.1371/journal.pgen.1006936. eCollection 2017 Jul. PubMed |
| IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells. Fu Y, Zhou Z, Wang H, Gong P, Guo R, Wang J, Lu X, Qi F, Liu L. FEBS Open Bio. 2017 Jun 29;7(8):1102-1110. doi: 10.1002/2211-5463.12246. eCollection 2017 Aug. PubMed |
| Identification and characterization of a long non-coding RNA up-regulated during HIV-1 infection. Postler TS, Pantry SN, Desrosiers RC, Ghosh S. Virology. 2017 Nov;511:30-39. doi: 10.1016/j.virol.2017.08.006. Epub 2017 Aug 10. PubMed |
| Open Chromatin Profiling in hiPSC-Derived Neurons Prioritizes Functional Noncoding Psychiatric Risk Variants and Highlights Neurodevelopmental Loci. Forrest MP, Zhang H, Moy W, McGowan H, Leites C, Dionisio LE, Xu Z, Shi J, Sanders AR, Greenleaf WJ, Cowan CA, Pang ZP, Gejman PV, Penzes P, Duan J. Cell Stem Cell. 2017 Sep 7;21(3):305-318.e8. doi: 10.1016/j.stem.2017.07.008. Epub 2017 Aug 10. PubMed |
| Sensitized mutagenesis screen in Factor V Leiden mice identifies thrombosis suppressor loci. Westrick RJ, Tomberg K, Siebert AE, Zhu G, Winn ME, Dobies SL, Manning SL, Brake MA, Cleuren AC, Hobbs LM, Mishack LM, Johnston AJ, Kotnik E, Siemieniak DR, Xu J, Li JZ, Saunders TL, Ginsburg D. Proc Natl Acad Sci U S A. 2017 Sep 5;114(36):9659-9664. doi: 10.1073/pnas.1705762114. Epub 2017 Aug 21. PubMed |
| SIRT7 antagonizes TGF-beta signaling and inhibits breast cancer metastasis. Tang X, Shi L, Xie N, Liu Z, Qian M, Meng F, Xu Q, Zhou M, Cao X, Zhu WG, Liu B. Nat Commun. 2017 Aug 22;8(1):318. doi: 10.1038/s41467-017-00396-9. PubMed |
| Lack of Trex1 Causes Systemic Autoimmunity despite the Presence of Antiretroviral Drugs. Achleitner M, Kleefisch M, Hennig A, Peschke K, Polikarpova A, Oertel R, Gabriel B, Schulze L, Lindeman D, Gerbaulet A, Fiebig U, Lee-Kirsch MA, Roers A, Behrendt R. J Immunol. 2017 Oct 1;199(7):2261-2269. doi: 10.4049/jimmunol.1700714. Epub 2017 Aug 23. PubMed |
| Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes. Ishizu T, Higo S, Masumura Y, Kohama Y, Shiba M, Higo T, Shibamoto M, Nakagawa A, Morimoto S, Takashima S, Hikoso S, Sakata Y. Sci Rep. 2017 Aug 24;7(1):9363. doi: 10.1038/s41598-017-09716-x. PubMed |
Postnatal Cardiac Gene Editing Using CRISPR/Cas9 With AAV9-Mediated Delivery of Short Guide RNAs Results in Mosaic Gene Disruption.
Johansen AK, Molenaar B, Versteeg D, Leitoguinho AR, Demkes C, Spanjaard B, de Ruiter H, Akbari Moqadam F, Kooijman L, Zentilin L, Giacca M, van Rooij E.
Circ Res. 2017 Oct 27;121(10):1168-1181. doi: 10.1161/CIRCRESAHA.116.310370. Epub 2017 Aug 29.
PubMed
Associated Plasmids |
| FMNL2 and -3 regulate Golgi architecture and anterograde transport downstream of Cdc42. Kage F, Steffen A, Ellinger A, Ranftler C, Gehre C, Brakebusch C, Pavelka M, Stradal T, Rottner K. Sci Rep. 2017 Aug 29;7(1):9791. doi: 10.1038/s41598-017-09952-1. PubMed |
| Proteolytic processing of lysyl oxidase-like-2 in the extracellular matrix is required for crosslinking of basement membrane collagen IV. Lopez-Jimenez AJ, Basak T, Vanacore RM. J Biol Chem. 2017 Oct 13;292(41):16970-16982. doi: 10.1074/jbc.M117.798603. Epub 2017 Sep 1. PubMed |
| Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity. Vujanovic M, Krietsch J, Raso MC, Terraneo N, Zellweger R, Schmid JA, Taglialatela A, Huang JW, Holland CL, Zwicky K, Herrador R, Jacobs H, Cortez D, Ciccia A, Penengo L, Lopes M. Mol Cell. 2017 Sep 7;67(5):882-890.e5. doi: 10.1016/j.molcel.2017.08.010. PubMed |
| CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression. Ren G, Jin W, Cui K, Rodrigez J, Hu G, Zhang Z, Larson DR, Zhao K. Mol Cell. 2017 Sep 21;67(6):1049-1058.e6. doi: 10.1016/j.molcel.2017.08.026. PubMed |
| Effects of genomic disruption of a guanine quadruplex in the 5' UTR of the Bcl-2 mRNA in melanoma cells. Serikawa T, Eberle J, Kurreck J. FEBS Lett. 2017 Nov;591(21):3649-3659. doi: 10.1002/1873-3468.12855. Epub 2017 Oct 3. PubMed |
| EHD Proteins Cooperate to Generate Caveolar Clusters and to Maintain Caveolae during Repeated Mechanical Stress. Yeow I, Howard G, Chadwick J, Mendoza-Topaz C, Hansen CG, Nichols BJ, Shvets E. Curr Biol. 2017 Oct 9;27(19):2951-2962.e5. doi: 10.1016/j.cub.2017.07.047. Epub 2017 Sep 21. PubMed |
| Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers. Yau EH, Kummetha IR, Lichinchi G, Tang R, Zhang Y, Rana TM. Cancer Res. 2017 Nov 15;77(22):6330-6339. doi: 10.1158/0008-5472.CAN-17-2043. Epub 2017 Sep 27. PubMed |
| RhoC regulates the actin remodeling required for phagosome formation during FcgammaR-mediated phagocytosis. Egami Y, Kawai K, Araki N. J Cell Sci. 2017 Dec 15;130(24):4168-4179. doi: 10.1242/jcs.202739. Epub 2017 Nov 7. PubMed |
| Galphai is required for carvedilol-induced beta1 adrenergic receptor beta-arrestin biased signaling. Wang J, Hanada K, Staus DP, Makara MA, Dahal GR, Chen Q, Ahles A, Engelhardt S, Rockman HA. Nat Commun. 2017 Nov 22;8(1):1706. doi: 10.1038/s41467-017-01855-z. PubMed |
| Comparative genomics reveals that loss of lunatic fringe (LFNG) promotes melanoma metastasis. Del Castillo Velasco-Herrera M, van der Weyden L, Nsengimana J, Speak AO, Sjoberg MK, Bishop DT, Jonsson G, Newton-Bishop J, Adams DJ. Mol Oncol. 2018 Feb;12(2):239-255. doi: 10.1002/1878-0261.12161. Epub 2018 Jan 7. PubMed |
| ATM and CDK2 control chromatin remodeler CSB to inhibit RIF1 in DSB repair pathway choice. Batenburg NL, Walker JR, Noordermeer SM, Moatti N, Durocher D, Zhu XD. Nat Commun. 2017 Dec 4;8(1):1921. doi: 10.1038/s41467-017-02114-x. PubMed |
| Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis. Takahara M, Katoh Y, Nakamura K, Hirano T, Sugawa M, Tsurumi Y, Nakayama K. Hum Mol Genet. 2018 Feb 1;27(3):516-528. doi: 10.1093/hmg/ddx421. PubMed |
| Therapeutic relevance of the PP2A-B55 inhibitory kinase MASTL/Greatwall in breast cancer. Alvarez-Fernandez M, Sanz-Flores M, Sanz-Castillo B, Salazar-Roa M, Partida D, Zapatero-Solana E, Ali HR, Manchado E, Lowe S, VanArsdale T, Shields D, Caldas C, Quintela-Fandino M, Malumbres M. Cell Death Differ. 2017 Dec 11. pii: 10.1038/s41418-017-0024-0. doi: 10.1038/s41418-017-0024-0. PubMed |
| Regulation of the cohesin-loading factor NIPBL: Role of the lncRNA NIPBL-AS1 and identification of a distal enhancer element. Zuin J, Casa V, Pozojevic J, Kolovos P, van den Hout MCGN, van Ijcken WFJ, Parenti I, Braunholz D, Baron Y, Watrin E, Kaiser FJ, Wendt KS. PLoS Genet. 2017 Dec 20;13(12):e1007137. doi: 10.1371/journal.pgen.1007137. eCollection 2017 Dec. PubMed |
A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes.
Bersuker K, Peterson CWH, To M, Sahl SJ, Savikhin V, Grossman EA, Nomura DK, Olzmann JA.
Dev Cell. 2018 Jan 8;44(1):97-112.e7. doi: 10.1016/j.devcel.2017.11.020. Epub 2017 Dec 21.
PubMed
Associated Plasmids |
| CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog. Pusapati GV, Kong JH, Patel BB, Krishnan A, Sagner A, Kinnebrew M, Briscoe J, Aravind L, Rohatgi R. Dev Cell. 2018 Jan 8;44(1):113-129.e8. doi: 10.1016/j.devcel.2017.12.003. Epub 2017 Dec 28. PubMed |
| ER-stress mobilization of death-associated protein kinase-1-dependent xenophagy counteracts mitochondria stress-induced epithelial barrier dysfunction. Lopes F, Keita AV, Saxena A, Reyes JL, Mancini NL, Al Rajabi A, Wang A, Baggio CH, Dicay M, van Dalen R, Ahn Y, Carneiro MBH, Peters NC, Rho JM, MacNaughton WK, Girardin SE, Jijon H, Philpott DJ, Soderholm JD, McKay DM. J Biol Chem. 2018 Mar 2;293(9):3073-3087. doi: 10.1074/jbc.RA117.000809. Epub 2018 Jan 9. PubMed |
| Cerebral organoids derived from Sandhoff disease-induced pluripotent stem cells exhibit impaired neurodifferentiation. Allende ML, Cook EK, Larman BC, Nugent A, Brady JM, Golebiowski D, Sena-Esteves M, Tifft CJ, Proia RL. J Lipid Res. 2018 Mar;59(3):550-563. doi: 10.1194/jlr.M081323. Epub 2018 Jan 22. PubMed |
| ERAD-dependent control of the Wnt secretory factor Evi. Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, Christianson JC, Boutros M. EMBO J. 2018 Feb 15;37(4). pii: embj.201797311. doi: 10.15252/embj.201797311. Epub 2018 Jan 29. PubMed |
MGA, L3MBTL2 and E2F6 determine genomic binding of the non-canonical Polycomb repressive complex PRC1.6.
Stielow B, Finkernagel F, Stiewe T, Nist A, Suske G.
PLoS Genet. 2018 Jan 30;14(1):e1007193. doi: 10.1371/journal.pgen.1007193.
PubMed
Associated Plasmids |
| PARP-1-dependent recruitment of cold-inducible RNA-binding protein promotes double-strand break repair and genome stability. Chen JK, Lin WL, Chen Z, Liu HW. Proc Natl Acad Sci U S A. 2018 Feb 20;115(8):E1759-E1768. doi: 10.1073/pnas.1713912115. Epub 2018 Feb 5. PubMed |
| ZBTB2 reads unmethylated CpG island promoters and regulates embryonic stem cell differentiation. Karemaker ID, Vermeulen M. EMBO Rep. 2018 Apr;19(4). pii: embr.201744993. doi: 10.15252/embr.201744993. Epub 2018 Feb 1. PubMed |
| DeSUMOylation switches Kaiso from activator to repressor upon hyperosmotic stress. Zhenilo S, Deyev I, Litvinova E, Zhigalova N, Kaplun D, Sokolov A, Mazur A, Prokhortchouk E. Cell Death Differ. 2018 Nov;25(11):1938-1951. doi: 10.1038/s41418-018-0078-7. Epub 2018 Feb 22. PubMed |
| Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1. Palstra RJ, de Crignis E, Roling MD, van Staveren T, Kan TW, van Ijcken W, Mueller YM, Katsikis PD, Mahmoudi T. Sci Adv. 2018 Feb 21;4(2):e1701729. doi: 10.1126/sciadv.1701729. eCollection 2018 Feb. PubMed |
| Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic alpha-helix. Burgess SG, Mukherjee M, Sabir S, Joseph N, Gutierrez-Caballero C, Richards MW, Huguenin-Dezot N, Chin JW, Kennedy EJ, Pfuhl M, Royle SJ, Gergely F, Bayliss R. EMBO J. 2018 Apr 13;37(8). pii: embj.201797902. doi: 10.15252/embj.201797902. Epub 2018 Mar 6. PubMed |
| A VCP inhibitor substrate trapping approach (VISTA) enables proteomic profiling of endogenous ERAD substrates. Huang EY, To M, Tran E, Dionisio LTA, Cho HJ, Baney KLM, Pataki CI, Olzmann JA. Mol Biol Cell. 2018 May 1;29(9):1021-1030. doi: 10.1091/mbc.E17-08-0514. Epub 2018 Mar 22. PubMed |
| Negative regulation of amino acid signaling by MAPK-regulated 4F2hc/Girdin complex. Weng L, Han YP, Enomoto A, Kitaura Y, Nagamori S, Kanai Y, Asai N, An J, Takagishi M, Asai M, Mii S, Masuko T, Shimomura Y, Takahashi M. PLoS Biol. 2018 Mar 14;16(3):e2005090. doi: 10.1371/journal.pbio.2005090. eCollection 2018 Mar. PubMed |
| ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability. Haahr P, Borgermann N, Guo X, Typas D, Achuthankutty D, Hoffmann S, Shearer R, Sixma TK, Mailand N. Mol Cell. 2018 Apr 5;70(1):165-174.e6. doi: 10.1016/j.molcel.2018.02.024. Epub 2018 Mar 22. PubMed |
| Phospholipid-flipping activity of P4-ATPase drives membrane curvature. Takada N, Naito T, Inoue T, Nakayama K, Takatsu H, Shin HW. EMBO J. 2018 May 2;37(9). pii: embj.201797705. doi: 10.15252/embj.201797705. Epub 2018 Mar 29. PubMed |
| Phosphorylation of Beta-3 adrenergic receptor at serine 247 by ERK MAP kinase drives lipolysis in obese adipocytes. Hong S, Song W, Zushin PH, Liu B, Jedrychowski MP, Mina AI, Deng Z, Cabarkapa D, Hall JA, Palmer CJ, Aliakbarian H, Szpyt J, Gygi SP, Tavakkoli A, Lynch L, Perrimon N, Banks AS. Mol Metab. 2018 Jun;12:25-38. doi: 10.1016/j.molmet.2018.03.012. Epub 2018 Mar 29. PubMed |
| Pluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity. Finley LWS, Vardhana SA, Carey BW, Alonso-Curbelo D, Koche R, Chen Y, Wen D, King B, Radler MR, Rafii S, Lowe SW, Allis CD, Thompson CB. Nat Cell Biol. 2018 May;20(5):565-574. doi: 10.1038/s41556-018-0086-3. Epub 2018 Apr 16. PubMed |
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| The Hippo Pathway Regulates Caveolae Expression and Mediates Flow Response via Caveolae. Rausch V, Bostrom JR, Park J, Bravo IR, Feng Y, Hay DC, Link BA, Hansen CG. Curr Biol. 2019 Jan 21;29(2):242-255.e6. doi: 10.1016/j.cub.2018.11.066. Epub 2018 Dec 27. PubMed |
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| HIF-1alpha and HIF-2alpha Differently Regulate the Radiation Sensitivity of NSCLC Cells. Moreno Roig E, Groot AJ, Yaromina A, Hendrickx TC, Barbeau LMO, Giuranno L, Dams G, Ient J, Olivo Pimentel V, van Gisbergen MW, Dubois LJ, Vooijs MA. Cells. 2019 Jan 12;8(1). pii: cells8010045. doi: 10.3390/cells8010045. PubMed |
| LEADeR role of miR-205 host gene as long noncoding RNA in prostate basal cell differentiation. Profumo V, Forte B, Percio S, Rotundo F, Doldi V, Ferrari E, Fenderico N, Dugo M, Romagnoli D, Benelli M, Valdagni R, Dolfini D, Zaffaroni N, Gandellini P. Nat Commun. 2019 Jan 18;10(1):307. doi: 10.1038/s41467-018-08153-2. PubMed |
| Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells. Fenderico N, van Scherpenzeel RC, Goldflam M, Proverbio D, Jordens I, Kralj T, Stryeck S, Bass TZ, Hermans G, Ullman C, Aastrup T, Gros P, Maurice MM. Nat Commun. 2019 Jan 21;10(1):365. doi: 10.1038/s41467-018-08172-z. PubMed |
| CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization. Hayward D, Alfonso-Perez T, Cundell MJ, Hopkins M, Holder J, Bancroft J, Hutter LH, Novak B, Barr FA, Gruneberg U. J Cell Biol. 2019 Apr 1;218(4):1182-1199. doi: 10.1083/jcb.201808014. Epub 2019 Jan 23. PubMed |
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| Analysis of Transcriptional Regulation in Bone Cells. Jin H, Sophocleous A, Azfer A, Ralston SH. Methods Mol Biol. 2019;1914:145-167. doi: 10.1007/978-1-4939-8997-3_8. PubMed |
| Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility. Yeshaw WM, van der Zwaag M, Pinto F, Lahaye LL, Faber AI, Gomez-Sanchez R, Dolga AM, Poland C, Monaco AP, van IJzendoorn SC, Grzeschik NA, Velayos-Baeza A, Sibon OC. Elife. 2019 Feb 11;8. pii: 43561. doi: 10.7554/eLife.43561. PubMed |
| SLFN11 can sensitize tumor cells towards IFN-gamma-mediated T cell killing. Mezzadra R, de Bruijn M, Jae LT, Gomez-Eerland R, Duursma A, Scheeren FA, Brummelkamp TR, Schumacher TN. PLoS One. 2019 Feb 12;14(2):e0212053. doi: 10.1371/journal.pone.0212053. eCollection 2019. PubMed |
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| Statins and Histone Deacetylase Inhibitors Affect Lamin A/C - Histone Deacetylase 2 Interaction in Human Cells. Mattioli E, Andrenacci D, Mai A, Valente S, Robijns J, De Vos WH, Capanni C, Lattanzi G. Front Cell Dev Biol. 2019 Jan 31;7:6. doi: 10.3389/fcell.2019.00006. eCollection 2019. PubMed |
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Associated Plasmids |
| Prolyl isomerization of FAAP20 catalyzed by PIN1 regulates the Fanconi anemia pathway. Wang J, Chan B, Tong M, Paung Y, Jo U, Martin D, Seeliger M, Haley J, Kim H. PLoS Genet. 2019 Feb 21;15(2):e1007983. doi: 10.1371/journal.pgen.1007983. eCollection 2019 Feb. PubMed |
| Differential genomic destabilisation in human cells with pathogenic MSH2 mutations introduced by genome editing. Hayashida G, Shioi S, Hidaka K, Fujikane R, Hidaka M, Tsurimoto T, Tsuzuki T, Oda S, Nakatsu Y. Exp Cell Res. 2019 Apr 15;377(1-2):24-35. doi: 10.1016/j.yexcr.2019.02.020. Epub 2019 Feb 22. PubMed |
| Human Fis1 regulates mitochondrial dynamics through inhibition of the fusion machinery. Yu R, Jin SB, Lendahl U, Nister M, Zhao J. EMBO J. 2019 Apr 15;38(8). pii: embj.201899748. doi: 10.15252/embj.201899748. Epub 2019 Mar 6. PubMed |
| CHD7 promotes glioblastoma cell motility and invasiveness through transcriptional modulation of an invasion signature. Machado RAC, Schneider H, DeOcesano-Pereira C, Lichtenstein F, Andrade F, Fujita A, Trombetta-Lima M, Weller M, Bowman-Colin C, Sogayar MC. Sci Rep. 2019 Mar 8;9(1):3952. doi: 10.1038/s41598-019-39564-w. PubMed |
| A complex between DYRK1A and DCAF7 phosphorylates the C-terminal domain of RNA polymerase II to promote myogenesis. Yu D, Cattoglio C, Xue Y, Zhou Q. Nucleic Acids Res. 2019 May 21;47(9):4462-4475. doi: 10.1093/nar/gkz162. PubMed |
| PKM2 coordinates glycolysis with mitochondrial fusion and oxidative phosphorylation. Li T, Han J, Jia L, Hu X, Chen L, Wang Y. Protein Cell. 2019 Aug;10(8):583-594. doi: 10.1007/s13238-019-0618-z. Epub 2019 Mar 18. PubMed |
| Construction of non-canonical PAM-targeting adenosine base editors by restriction enzyme-free DNA cloning using CRISPR-Cas9. Jeong YK, Yu J, Bae S. Sci Rep. 2019 Mar 20;9(1):4939. doi: 10.1038/s41598-019-41356-1. PubMed |
| Oncogenic PIK3CA promotes cellular stemness in an allele dose-dependent manner. Madsen RR, Knox RG, Pearce W, Lopez S, Mahler-Araujo B, McGranahan N, Vanhaesebroeck B, Semple RK. Proc Natl Acad Sci U S A. 2019 Apr 23;116(17):8380-8389. doi: 10.1073/pnas.1821093116. Epub 2019 Apr 4. PubMed |
| A role for Rab11 in the homeostasis of the endosome-lysosomal pathway. Zulkefli KL, Houghton FJ, Gosavi P, Gleeson PA. Exp Cell Res. 2019 Jul 1;380(1):55-68. doi: 10.1016/j.yexcr.2019.04.010. Epub 2019 Apr 11. PubMed |
| LC8/DYNLL1 is a 53BP1 effector and regulates checkpoint activation. West KL, Kelliher JL, Xu Z, An L, Reed MR, Eoff RL, Wang J, Huen MSY, Leung JWC. Nucleic Acids Res. 2019 Jul 9;47(12):6236-6249. doi: 10.1093/nar/gkz263. PubMed |
| Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns. Alateeq S, Ovchinnikov D, Tracey T, Whitworth D, Al-Rubaish A, Al-Ali A, Wolvetang E. APL Bioeng. 2018 Dec 3;2(4):046103. doi: 10.1063/1.5048625. eCollection 2018 Dec. PubMed |
| GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor. Jewell JL, Fu V, Hong AW, Yu FX, Meng D, Melick CH, Wang H, Lam WM, Yuan HX, Taylor SS, Guan KL. Elife. 2019 May 21;8. pii: 43038. doi: 10.7554/eLife.43038. PubMed |
| The BBSome in POMC and AgRP Neurons Is Necessary for Body Weight Regulation and Sorting of Metabolic Receptors. Guo DF, Lin Z, Wu Y, Searby C, Thedens DR, Richerson GB, Usachev YM, Grobe JL, Sheffield VC, Rahmouni K. Diabetes. 2019 Aug;68(8):1591-1603. doi: 10.2337/db18-1088. Epub 2019 May 24. PubMed |
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| RB1 Deletion in Retinoblastoma Protein Pathway-Disrupted Cells Results in DNA Damage and Cancer Progression. Marshall AE, Roes MV, Passos DT, DeWeerd MC, Chaikovsky AC, Sage J, Howlett CJ, Dick FA. Mol Cell Biol. 2019 Jul 29;39(16). pii: MCB.00105-19. doi: 10.1128/MCB.00105-19. Print 2019 Aug 15. PubMed |
| Involvement of Interferon Regulatory Factor 7 in Nicotine's Suppression of Antiviral Immune Responses. Han H, Huang W, Du W, Shen Q, Yang Z, Li MD, Chang SL. J Neuroimmune Pharmacol. 2019 Jun 1. pii: 10.1007/s11481-019-09845-2. doi: 10.1007/s11481-019-09845-2. PubMed |
| Phosphorylation of FANCD2 Inhibits the FANCD2/FANCI Complex and Suppresses the Fanconi Anemia Pathway in the Absence of DNA Damage. Lopez-Martinez D, Kupculak M, Yang D, Yoshikawa Y, Liang CC, Wu R, Gygi SP, Cohn MA. Cell Rep. 2019 Jun 4;27(10):2990-3005.e5. doi: 10.1016/j.celrep.2019.05.003. PubMed |
| ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint. Yuan F, Jin X, Li D, Song Y, Zhang N, Yang X, Wang L, Zhu WG, Tian C, Zhao Y. Nucleic Acids Res. 2019 Sep 5;47(15):8096-8110. doi: 10.1093/nar/gkz602. PubMed |
| Acetylation of BMAL1 by TIP60 controls BRD4-P-TEFb recruitment to circadian promoters. Petkau N, Budak H, Zhou X, Oster H, Eichele G. Elife. 2019 Jul 11;8. pii: 43235. doi: 10.7554/eLife.43235. PubMed |
| Toxoplasma GRA15 Activates the NF-kappaB Pathway through Interactions with TNF Receptor-Associated Factors. Sangare LO, Yang N, Konstantinou EK, Lu D, Mukhopadhyay D, Young LH, Saeij JPJ. mBio. 2019 Jul 16;10(4). pii: mBio.00808-19. doi: 10.1128/mBio.00808-19. PubMed |
| The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection. Moskovskich A, Goldmann U, Kartnig F, Lindinger S, Konecka J, Fiume G, Girardi E, Superti-Furga G. Sci Rep. 2019 Jul 18;9(1):10471. doi: 10.1038/s41598-019-46952-9. PubMed |
| Conditional deletion of glucocorticoid receptors in rat brain results in sex-specific deficits in fear and coping behaviors. Scheimann JR, Moloney RD, Mahbod P, Morano RL, Fitzgerald M, Hoskins O, Packard BA, Cotella EM, Hu YC, Herman JP. Elife. 2019 Jul 22;8. pii: 44672. doi: 10.7554/eLife.44672. PubMed |
| SMYD2 Drives Mesendodermal Differentiation of Human Embryonic Stem Cells Through Mediating the Transcriptional Activation of Key Mesendodermal Genes. Bai HJ, Zhang P, Ma L, Liang H, Wei G, Yang HT. Stem Cells. 2019 Nov;37(11):1401-1415. doi: 10.1002/stem.3068. Epub 2019 Aug 12. PubMed |
The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive Effects of LKB1 in NSCLC.
Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ.
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| Gene-edited murine cell lines for propagation of chronic wasting disease prions. Walia R, Ho CC, Lee C, Gilch S, Schatzl HM. Sci Rep. 2019 Aug 1;9(1):11151. doi: 10.1038/s41598-019-47629-z. PubMed |
| Autokinase Activity of Casein Kinase 1 delta/epsilon Governs the Period of Mammalian Circadian Rhythms. Guo G, Wang K, Hu SS, Tian T, Liu P, Mori T, Chen P, Johnson CH, Qin X. J Biol Rhythms. 2019 Aug 8:748730419865406. doi: 10.1177/0748730419865406. PubMed |
| The synthesis of branched-chain fatty acids is limited by enzymatic decarboxylation of ethyl- and methylmalonyl-CoA. Dewulf JP, Gerin I, Rider MH, Veiga-da-Cunha M, Van Schaftingen E, Bommer GT. Biochem J. 2019 Aug 30;476(16):2427-2447. doi: 10.1042/BCJ20190500. PubMed |
| Single-Cell Analysis of Multiple Steps of Dynamic NF-kappaB Regulation in Interleukin-1alpha-Triggered Tumor Cells Using Proximity Ligation Assays. Mayr-Buro C, Schlereth E, Beuerlein K, Tenekeci U, Meier-Soelch J, Schmitz ML, Kracht M. Cancers (Basel). 2019 Aug 16;11(8). pii: cancers11081199. doi: 10.3390/cancers11081199. PubMed |
| Regulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing system. Kang JG, Park JS, Ko JH, Kim YS. Sci Rep. 2019 Aug 19;9(1):11960. doi: 10.1038/s41598-019-48130-3. PubMed |
| Attenuation of the Innate Immune Response against Viral Infection Due to ZNF598-Promoted Binding of FAT10 to RIG-I. Wang G, Kouwaki T, Okamoto M, Oshiumi H. Cell Rep. 2019 Aug 20;28(8):1961-1970.e4. doi: 10.1016/j.celrep.2019.07.081. PubMed |
A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology.
Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, Nakamura M, Sasaki E, Shiozawa S, Okano H.
PLoS One. 2019 Aug 27;14(8):e0221164. doi: 10.1371/journal.pone.0221164. eCollection 2019.
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| Transcriptomic analyses of gene expression by CRISPR knockout of miR-214 in cervical cancer cells. Sen P, Ghosal S, Hazra R, Arega S, Mohanty R, Kulkarni KK, Budhwar R, Ganguly N. Genomics. 2020 Mar;112(2):1490-1499. doi: 10.1016/j.ygeno.2019.08.020. Epub 2019 Aug 26. PubMed |
| Transcriptional Responses to IFN-gamma Require Mediator Kinase-Dependent Pause Release and Mechanistically Distinct CDK8 and CDK19 Functions. Steinparzer I, Sedlyarov V, Rubin JD, Eislmayr K, Galbraith MD, Levandowski CB, Vcelkova T, Sneezum L, Wascher F, Amman F, Kleinova R, Bender H, Andrysik Z, Espinosa JM, Superti-Furga G, Dowell RD, Taatjes DJ, Kovarik P. Mol Cell. 2019 Nov 7;76(3):485-499.e8. doi: 10.1016/j.molcel.2019.07.034. Epub 2019 Sep 5. PubMed |
| Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity. Gemble S, Simon A, Pennetier C, Dumont M, Herve S, Meitinger F, Oegema K, Rodriguez R, Almouzni G, Fachinetti D, Basto R. Curr Biol. 2019 Sep 23;29(18):3072-3080.e5. doi: 10.1016/j.cub.2019.07.052. Epub 2019 Sep 5. PubMed |
| Telomere dysfunction impairs epidermal stem cell specification and differentiation by disrupting BMP/pSmad/P63 signaling. Liu N, Yin Y, Wang H, Zhou Z, Sheng X, Fu H, Guo R, Wang H, Yang J, Gong P, Ning W, Ju Z, Liu Y, Liu L. PLoS Genet. 2019 Sep 13;15(9):e1008368. doi: 10.1371/journal.pgen.1008368. eCollection 2019 Sep. PubMed |
| Mouse Tryptase Gene Expression is Coordinately Regulated by GATA1 and GATA2 in Bone Marrow-Derived Mast Cells. Ohneda K, Ohmori S, Yamamoto M. Int J Mol Sci. 2019 Sep 17;20(18). pii: ijms20184603. doi: 10.3390/ijms20184603. PubMed |
| Human GLB1 knockout cerebral organoids: A model system for testing AAV9-mediated GLB1 gene therapy for reducing GM1 ganglioside storage in GM1 gangliosidosis. Latour YL, Yoon R, Thomas SE, Grant C, Li C, Sena-Esteves M, Allende ML, Proia RL, Tifft CJ. Mol Genet Metab Rep. 2019 Sep 11;21:100513. doi: 10.1016/j.ymgmr.2019.100513. eCollection 2019 Dec. PubMed |
| Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Laflamme C, McKeever PM, Kumar R, Schwartz J, Kolahdouzan M, Chen CX, You Z, Benaliouad F, Gileadi O, McBride HM, Durcan TM, Edwards AM, Healy LM, Robertson J, McPherson PS. Elife. 2019 Oct 15;8. pii: 48363. doi: 10.7554/eLife.48363. PubMed |
| Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids. Chhabra S, Liu L, Goh R, Kong X, Warmflash A. PLoS Biol. 2019 Oct 15;17(10):e3000498. doi: 10.1371/journal.pbio.3000498. eCollection 2019 Oct. PubMed |
| The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis. Bersuker K, Hendricks JM, Li Z, Magtanong L, Ford B, Tang PH, Roberts MA, Tong B, Maimone TJ, Zoncu R, Bassik MC, Nomura DK, Dixon SJ, Olzmann JA. Nature. 2019 Nov;575(7784):688-692. doi: 10.1038/s41586-019-1705-2. Epub 2019 Oct 21. PubMed |
| 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination. Callen E, Zong D, Wu W, Wong N, Stanlie A, Ishikawa M, Pavani R, Dumitrache LC, Byrum AK, Mendez-Dorantes C, Martinez P, Canela A, Maman Y, Day A, Kruhlak MJ, Blasco MA, Stark JM, Mosammaparast N, McKinnon PJ, Nussenzweig A. Mol Cell. 2020 Jan 2;77(1):26-38.e7. doi: 10.1016/j.molcel.2019.09.024. Epub 2019 Oct 22. PubMed |
| Pyridoxamine-phosphate oxidases and pyridoxamine-phosphate oxidase-related proteins catalyze the oxidation of 6-NAD(P)H to NAD(P). Marbaix AY, Chehade G, Noel G, Morsomme P, Vertommen D, Bommer GT, Van Schaftingen E. Biochem J. 2019 Oct 30;476(20):3033-3052. doi: 10.1042/BCJ20190602. PubMed |
| Distinct IL-1alpha-responsive enhancers promote acute and coordinated changes in chromatin topology in a hierarchical manner. Weiterer SS, Meier-Soelch J, Georgomanolis T, Mizi A, Beyerlein A, Weiser H, Brant L, Mayr-Buro C, Jurida L, Beuerlein K, Muller H, Weber A, Tenekeci U, Dittrich-Breiholz O, Bartkuhn M, Nist A, Stiewe T, van IJcken WF, Riedlinger T, Schmitz ML, Papantonis A, Kracht M. EMBO J. 2020 Jan 2;39(1):e101533. doi: 10.15252/embj.2019101533. Epub 2019 Nov 7. PubMed |
| The role of CXC receptors signaling in early stages of mouse embryonic stem cell differentiation. Kowalski K, Brzoska E, Ciemerych MA. Stem Cell Res. 2019 Dec;41:101636. doi: 10.1016/j.scr.2019.101636. Epub 2019 Oct 31. PubMed |
Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex.
Naito T, Ercan B, Krshnan L, Triebl A, Koh DHZ, Wei FY, Tomizawa K, Torta FT, Wenk MR, Saheki Y.
Elife. 2019 Nov 14;8. pii: 51401. doi: 10.7554/eLife.51401.
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| Inhibitor of Growth 4 (ING4) is a positive regulator of rRNA synthesis. Trinh DA, Shirakawa R, Kimura T, Sakata N, Goto K, Horiuchi H. Sci Rep. 2019 Nov 21;9(1):17235. doi: 10.1038/s41598-019-53767-1. PubMed |
| Potent Antitumor Activity of Liposomal Irinotecan in an Organoid- and CRISPR-Cas9-Based Murine Model of Gallbladder Cancer. Erlangga Z, Wolff K, Poth T, Peltzer A, Nahnsen S, Spielberg S, Timrott K, Woller N, Kuhnel F, Manns MP, Saborowski A, Vogel A, Saborowski M. Cancers (Basel). 2019 Nov 29;11(12). pii: cancers11121904. doi: 10.3390/cancers11121904. PubMed |
| Lack of the multidrug transporter MRP4/ABCC4 defines the PEL-negative blood group and impairs platelet aggregation. Azouzi S, Mikdar M, Hermand P, Gautier EF, Salnot V, Willemetz A, Nicolas G, Vrignaud C, Raneri A, Mayeux P, Bole-Feysot C, Nitschke P, Cartron JP, Colin Y, Hermine O, Jedlitschky G, Cloutier M, Constanzo-Yanez J, Ethier C, Robitaille N, St-Louis M, Le Van Kim C, Peyrard T. Blood. 2020 Feb 6;135(6):441-448. doi: 10.1182/blood.2019002320. PubMed |
| Identification of ncRNA-Mediated Functions of Nucleus-Localized miR-320 in Cardiomyocytes. Li H, Zhan J, Zhao Y, Fan J, Yuan S, Yin Z, Dai B, Chen C, Wang DW. Mol Ther Nucleic Acids. 2019 Nov 18;19:132-143. doi: 10.1016/j.omtn.2019.11.006. PubMed |
| The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Leidal AM, Huang HH, Marsh T, Solvik T, Zhang D, Ye J, Kai F, Goldsmith J, Liu JY, Huang YH, Monkkonen T, Vlahakis A, Huang EJ, Goodarzi H, Yu L, Wiita AP, Debnath J. Nat Cell Biol. 2020 Feb;22(2):187-199. doi: 10.1038/s41556-019-0450-y. Epub 2020 Jan 13. PubMed |
| YB-3 substitutes YB-1 in global mRNA binding. Lyabin DN, Eliseeva IA, Smolin EA, Doronin AN, Budkina KS, Kulakovskiy IV, Ovchinnikov LP. RNA Biol. 2020 Apr;17(4):487-499. doi: 10.1080/15476286.2019.1710050. Epub 2020 Jan 16. PubMed |
| Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells. Rhodes JDP, Feldmann A, Hernandez-Rodriguez B, Diaz N, Brown JM, Fursova NA, Blackledge NP, Prathapan P, Dobrinic P, Huseyin MK, Szczurek A, Kruse K, Nasmyth KA, Buckle VJ, Vaquerizas JM, Klose RJ. Cell Rep. 2020 Jan 21;30(3):820-835.e10. doi: 10.1016/j.celrep.2019.12.057. PubMed |
| The mitochondrial HSP90 paralog TRAP1 forms an OXPHOS-regulated tetramer and is involved in mitochondrial metabolic homeostasis. Joshi A, Dai L, Liu Y, Lee J, Ghahhari NM, Segala G, Beebe K, Jenkins LM, Lyons GC, Bernasconi L, Tsai FTF, Agard DA, Neckers L, Picard D. BMC Biol. 2020 Jan 27;18(1):10. doi: 10.1186/s12915-020-0740-7. PubMed |
| The role of Mediator and Little Elongation Complex in transcription termination. Takahashi H, Ranjan A, Chen S, Suzuki H, Shibata M, Hirose T, Hirose H, Sasaki K, Abe R, Chen K, He Y, Zhang Y, Takigawa I, Tsukiyama T, Watanabe M, Fujii S, Iida M, Yamamoto J, Yamaguchi Y, Suzuki Y, Matsumoto M, Nakayama KI, Washburn MP, Saraf A, Florens L, Sato S, Tomomori-Sato C, Conaway RC, Conaway JW, Hatakeyama S. Nat Commun. 2020 Feb 26;11(1):1063. doi: 10.1038/s41467-020-14849-1. PubMed |
| Critical roles of phosphoinositides and NF2 in Hippo pathway regulation. Hong AW, Meng Z, Plouffe SW, Lin Z, Zhang M, Guan KL. Genes Dev. 2020 Apr 1;34(7-8):511-525. doi: 10.1101/gad.333435.119. Epub 2020 Feb 27. PubMed |
| Polycomb group RING finger protein 5 influences several developmental signaling pathways during the in vitro differentiation of mouse embryonic stem cells. Meng Y, Liu Y, Dakou E, Gutierrez GJ, Leyns L. Dev Growth Differ. 2020 May;62(4):232-242. doi: 10.1111/dgd.12659. Epub 2020 Apr 22. PubMed |
| Identification of the Repressive Domain of the Negative Circadian Clock Component CHRONO. Yang Y, Li N, Qiu J, Ge H, Qin X. Int J Mol Sci. 2020 Apr 2;21(7). pii: ijms21072469. doi: 10.3390/ijms21072469. PubMed |
| LPIAT1/MBOAT7 depletion increases triglyceride synthesis fueled by high phosphatidylinositol turnover. Tanaka Y, Shimanaka Y, Caddeo A, Kubo T, Mao Y, Kubota T, Kubota N, Yamauchi T, Mancina RM, Baselli G, Luukkonen P, Pihlajamaki J, Yki-Jarvinen H, Valenti L, Arai H, Romeo S, Kono N. Gut. 2021 Jan;70(1):180-193. doi: 10.1136/gutjnl-2020-320646. Epub 2020 Apr 6. PubMed |
| CRISPR/CAS9-mediated knockout of Abi1 inhibits p185(Bcr-Abl)-induced leukemogenesis and signal transduction to ERK and PI3K/Akt pathways. Faulkner J, Jiang P, Farris D, Walker R, Dai Z. J Hematol Oncol. 2020 Apr 10;13(1):34. doi: 10.1186/s13045-020-00867-5. PubMed |
| TDP2 suppresses genomic instability induced by androgens in the epithelial cells of prostate glands. Al Mahmud MR, Ishii K, Bernal-Lozano C, Delgado-Sainz I, Toi M, Akamatsu S, Fukumoto M, Watanabe M, Takeda S, Cortes-Ledesma F, Sasanuma H. Genes Cells. 2020 Jul;25(7):450-465. doi: 10.1111/gtc.12770. Epub 2020 May 5. PubMed |
| Nek2-mediated GAS2L1 phosphorylation and centrosome-linker disassembly induce centrosome disjunction. Au FKC, Hau BKT, Qi RZ. J Cell Biol. 2020 May 4;219(5). pii: 151657. doi: 10.1083/jcb.201909094. PubMed |
| Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration. Kim Y, Lee J, Seppala M, Cobourne MT, Kim SH. Nat Commun. 2020 Apr 24;11(1):1994. doi: 10.1038/s41467-020-15897-3. PubMed |
| Loss of calpains-1 and -2 prevents repair of plasma membrane scrape injuries, but not small pores, and induces a severe muscular dystrophy. Piper AK, Sophocleous RA, Ross SE, Evesson FJ, Saleh O, Bournazos A, Yasa J, Reed C, Woolger N, Sluyter R, Greer P, Biro M, Lemckert FA, Cooper ST. Am J Physiol Cell Physiol. 2020 Jun 1;318(6):C1226-C1237. doi: 10.1152/ajpcell.00408.2019. Epub 2020 Apr 29. PubMed |
| Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. Hegarat N, Crncec A, Suarez Peredo Rodriguez MF, Echegaray Iturra F, Gu Y, Busby O, Lang PF, Barr AR, Bakal C, Kanemaki MT, Lamond AI, Novak B, Ly T, Hochegger H. EMBO J. 2020 Jun 2;39(11):e104419. doi: 10.15252/embj.2020104419. Epub 2020 Apr 30. PubMed |
| Human pluripotent stem cell-based models suggest preadipocyte senescence as a possible cause of metabolic complications of Werner and Bloom Syndromes. Goh KJ, Chen JH, Rocha N, Semple RK. Sci Rep. 2020 May 4;10(1):7490. doi: 10.1038/s41598-020-64136-8. PubMed |
| AKT Ser/Thr kinase increases V-ATPase-dependent lysosomal acidification in response to amino acid starvation in mammalian cells. Collins MP, Stransky LA, Forgac M. J Biol Chem. 2020 Jul 10;295(28):9433-9444. doi: 10.1074/jbc.RA120.013223. Epub 2020 May 14. PubMed |
| HDAC1 inhibition ameliorates TDP-43-induced cell death in vitro and in vivo. Sanna S, Esposito S, Masala A, Sini P, Nieddu G, Galioto M, Fais M, Iaccarino C, Cestra G, Crosio C. Cell Death Dis. 2020 May 14;11(5):369. doi: 10.1038/s41419-020-2580-3. PubMed |
| Histone H2A variants alpha1-extension helix directs RNF168-mediated ubiquitination. Kelliher JL, West KL, Gong Q, Leung JWC. Nat Commun. 2020 May 18;11(1):2462. doi: 10.1038/s41467-020-16307-4. PubMed |
| HLTF Promotes Fork Reversal, Limiting Replication Stress Resistance and Preventing Multiple Mechanisms of Unrestrained DNA Synthesis. Bai G, Kermi C, Stoy H, Schiltz CJ, Bacal J, Zaino AM, Hadden MK, Eichman BF, Lopes M, Cimprich KA. Mol Cell. 2020 Jun 18;78(6):1237-1251.e7. doi: 10.1016/j.molcel.2020.04.031. Epub 2020 May 21. PubMed |
| TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes. Liang H, Zhang P, Bai HJ, Huang J, Yang HT. Stem Cell Res Ther. 2020 May 24;11(1):196. doi: 10.1186/s13287-020-01711-w. PubMed |
| Role of CD133 in human embryonic stem cell proliferation and teratoma formation. Wang H, Gong P, Li J, Fu Y, Zhou Z, Liu L. Stem Cell Res Ther. 2020 May 27;11(1):208. doi: 10.1186/s13287-020-01729-0. PubMed |
| Host cell factors stimulate HIV-1 transcription by antagonizing substrate-binding function of Siah1 ubiquitin ligase to stabilize transcription elongation factor ELL2. Wu J, Xue Y, Gao X, Zhou Q. Nucleic Acids Res. 2020 Jul 27;48(13):7321-7332. doi: 10.1093/nar/gkaa461. PubMed |
| HOXA5 inhibits the proliferation and neoplasia of cervical cancer cells via downregulating the activity of the Wnt/beta-catenin pathway and transactivating TP53. Ma HM, Cui N, Zheng PS. Cell Death Dis. 2020 Jun 4;11(6):420. doi: 10.1038/s41419-020-2629-3. PubMed |
| Transcriptional consequences of MBD5 disruption in mouse brain and CRISPR-derived neurons. Seabra CM, Aneichyk T, Erdin S, Tai DJC, De Esch CEF, Razaz P, An Y, Manavalan P, Ragavendran A, Stortchevoi A, Abad C, Young JI, Maciel P, Talkowski ME, Gusella JF. Mol Autism. 2020 Jun 5;11(1):45. doi: 10.1186/s13229-020-00354-1. PubMed |
| Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin-BAF Interaction and BAF Nuclear Localization. Cenni V, Squarzoni S, Loi M, Mattioli E, Lattanzi G, Capanni C. Cells. 2020 Jun 6;9(6). pii: cells9061415. doi: 10.3390/cells9061415. PubMed |
| TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes. Wetzel L, Blanchard S, Rama S, Beier V, Kaufmann A, Wollert T. Nat Commun. 2020 Jun 12;11(1):2993. doi: 10.1038/s41467-020-16689-5. PubMed |
| Synergy between SIRT1 and SIRT6 helps recognize DNA breaks and potentiates the DNA damage response and repair in humans and mice. Meng F, Qian M, Peng B, Peng L, Wang X, Zheng K, Liu Z, Tang X, Zhang S, Sun S, Cao X, Pang Q, Zhao B, Ma W, Songyang Z, Xu B, Zhu WG, Xu X, Liu B. Elife. 2020 Jun 15;9. pii: 55828. doi: 10.7554/eLife.55828. PubMed |
| Sequential CRISPR-Based Screens Identify LITAF and CDIP1 as the Bacillus cereus Hemolysin BL Toxin Host Receptors. Liu J, Zuo Z, Sastalla I, Liu C, Jang JY, Sekine Y, Li Y, Pirooznia M, Leppla SH, Finkel T, Liu S. Cell Host Microbe. 2020 Sep 9;28(3):402-410.e5. doi: 10.1016/j.chom.2020.05.012. Epub 2020 Jun 15. PubMed |
| Tagging and Deleting of Endogenous Caveolar Components Using CRISPR/Cas9 Technology. Shvets E, Mendoza-Topaz C. Methods Mol Biol. 2020;2169:149-166. doi: 10.1007/978-1-0716-0732-9_14. PubMed |
| CRISPR-Cas9 generated Pompe knock-in murine model exhibits early-onset hypertrophic cardiomyopathy and skeletal muscle weakness. Huang JY, Kan SH, Sandfeld EK, Dalton ND, Rangel AD, Chan Y, Davis-Turak J, Neumann J, Wang RY. Sci Rep. 2020 Jun 25;10(1):10321. doi: 10.1038/s41598-020-65259-8. PubMed |
| Loss of function of the mitochondrial peptidase PITRM1 induces proteotoxic stress and Alzheimer's disease-like pathology in human cerebral organoids. Perez MJ, Ivanyuk D, Panagiotakopoulou V, Di Napoli G, Kalb S, Brunetti D, Al-Shaana R, Kaeser SA, Fraschka SA, Jucker M, Zeviani M, Viscomi C, Deleidi M. Mol Psychiatry. 2020 Jul 7. pii: 10.1038/s41380-020-0807-4. doi: 10.1038/s41380-020-0807-4. PubMed |
| WRNIP1 Is Recruited to DNA Interstrand Crosslinks and Promotes Repair. Socha A, Yang D, Bulsiewicz A, Yaprianto K, Kupculak M, Liang CC, Hadjicharalambous A, Wu R, Gygi SP, Cohn MA. Cell Rep. 2020 Jul 7;32(1):107850. doi: 10.1016/j.celrep.2020.107850. PubMed |
| WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge. Tang Q, Schaks M, Koundinya N, Yang C, Pollard LW, Svitkina TM, Rottner K, Goode BL. Mol Biol Cell. 2020 Sep 15;31(20):2168-2178. doi: 10.1091/mbc.E19-12-0705. Epub 2020 Jul 22. PubMed |
| Gravin-associated kinase signaling networks coordinate gamma-tubulin organization at mitotic spindle poles. Bucko PJ, Garcia I, Manocha R, Bhat A, Wordeman L, Scott JD. J Biol Chem. 2020 Oct 2;295(40):13784-13797. doi: 10.1074/jbc.RA120.014791. Epub 2020 Jul 30. PubMed |
| Cell-substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime. Singh SP, Thomason PA, Lilla S, Schaks M, Tang Q, Goode BL, Machesky LM, Rottner K, Insall RH. PLoS Biol. 2020 Aug 3;18(8):e3000774. doi: 10.1371/journal.pbio.3000774. eCollection 2020 Aug. PubMed |
Loss of the Essential Autophagy Regulators FIP200 or Atg5 Leads to Distinct Effects on Focal Adhesion Composition and Organization.
Assar EA, Tumbarello DA.
Front Cell Dev Biol. 2020 Aug 4;8:733. doi: 10.3389/fcell.2020.00733. eCollection 2020.
PubMed
Associated Plasmids |
| DENR promotes translation reinitiation via ribosome recycling to drive expression of oncogenes including ATF4. Bohlen J, Harbrecht L, Blanco S, Clemm von Hohenberg K, Fenzl K, Kramer G, Bukau B, Teleman AA. Nat Commun. 2020 Sep 16;11(1):4676. doi: 10.1038/s41467-020-18452-2. PubMed |
| Cell-cycle-gated feedback control mediates desensitization to interferon stimulation. Mudla A, Jiang Y, Arimoto KI, Xu B, Rajesh A, Ryan AP, Wang W, Daugherty MD, Zhang DE, Hao N. Elife. 2020 Sep 18;9. pii: 58825. doi: 10.7554/eLife.58825. PubMed |
| Adeno-associated virus-mediated gene delivery promotes S-phase entry-independent precise targeted integration in cardiomyocytes. Kohama Y, Higo S, Masumura Y, Shiba M, Kondo T, Ishizu T, Higo T, Nakamura S, Kameda S, Tabata T, Inoue H, Motooka D, Okuzaki D, Takashima S, Miyagawa S, Sawa Y, Hikoso S, Sakata Y. Sci Rep. 2020 Sep 18;10(1):15348. doi: 10.1038/s41598-020-72216-y. PubMed |
| Differential transcriptome analysis in HPV-positive and HPV-negative cervical cancer cells through CRISPR knockout of miR-214. Sen P, Ganguly P, Kulkarni KK, Budhwar R, Ganguly N. J Biosci. 2020;45. PubMed |
| A Thalamic Orphan Receptor Drives Variability in Short-Term Memory. Hsiao K, Noble C, Pitman W, Yadav N, Kumar S, Keele GR, Terceros A, Kanke M, Conniff T, Cheleuitte-Nieves C, Tolwani R, Sethupathy P, Rajasethupathy P. Cell. 2020 Oct 15;183(2):522-536.e19. doi: 10.1016/j.cell.2020.09.011. Epub 2020 Sep 29. PubMed |
| Pathogenic mutations reveal a role of RECQ4 in mitochondrial RNA:DNA hybrid formation and resolution. Chang CW, Xu X, Li M, Xin D, Ding L, Wang YT, Liu Y. Sci Rep. 2020 Oct 12;10(1):17033. doi: 10.1038/s41598-020-74095-9. PubMed |
| Leptin Receptor Signaling Regulates Protein Synthesis Pathways and Neuronal Differentiation in Pluripotent Stem Cells. Gupta MK, Vethe H, Softic S, Rao TN, Wagh V, Shirakawa J, Barsnes H, Vaudel M, Takatani T, Kahraman S, Sakaguchi M, Martinez R, Hu J, Bjorlykke Y, Raeder H, Kulkarni RN. Stem Cell Reports. 2020 Nov 10;15(5):1067-1079. doi: 10.1016/j.stemcr.2020.10.001. Epub 2020 Oct 29. PubMed |
| Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT). Lee NE, Kim SH, Yu DY, Woo EJ, Kim MI, Seong GS, Lee SM, Ko JH, Kim YS. Commun Biol. 2020 Oct 30;3(1):636. doi: 10.1038/s42003-020-01363-9. PubMed |
| Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the CRB1 locus. Tang X, Chen Z, Tan X, Luo L, Liu X, Gong L, Li DW, Liu Y. Stem Cell Res. 2020 Dec;49:102057. doi: 10.1016/j.scr.2020.102057. Epub 2020 Oct 16. PubMed |
| Macrolide antibiotics enhance the antitumor effect of lansoprazole resulting in lysosomal membrane permeabilizationassociated cell death. Takeda A, Takano N, Kokuba H, Hino H, Moriya S, Abe A, Hiramoto M, Tsukahara K, Miyazawa K. Int J Oncol. 2020 Dec;57(6):1280-1292. doi: 10.3892/ijo.2020.5138. Epub 2020 Oct 21. PubMed |
| Two replication fork remodeling pathways generate nuclease substrates for distinct fork protection factors. Liu W, Krishnamoorthy A, Zhao R, Cortez D. Sci Adv. 2020 Nov 13;6(46). pii: 6/46/eabc3598. doi: 10.1126/sciadv.abc3598. Print 2020 Nov. PubMed |
| Identification of BAG2 and Cathepsin D as Plasma Biomarkers for Parkinson's Disease. Kang J, Kim JW, Heo H, Lee J, Park KY, Yoon JH, Chang J. Clin Transl Sci. 2021 Mar;14(2):606-616. doi: 10.1111/cts.12920. Epub 2020 Nov 22. PubMed |
| The Hsp70-Hsp90 co-chaperone Hop/Stip1 shifts the proteostatic balance from folding towards degradation. Bhattacharya K, Weidenauer L, Luengo TM, Pieters EC, Echeverria PC, Bernasconi L, Wider D, Sadian Y, Koopman MB, Villemin M, Bauer C, Rudiger SGD, Quadroni M, Picard D. Nat Commun. 2020 Nov 25;11(1):5975. doi: 10.1038/s41467-020-19783-w. PubMed |
| Wnt10b-GSK3beta-dependent Wnt/STOP signaling prevents aneuploidy in human somatic cells. Lin YC, Haas A, Bufe A, Parbin S, Hennecke M, Voloshanenko O, Gross J, Boutros M, Acebron SP, Bastians H. Life Sci Alliance. 2020 Nov 30;4(1). pii: 4/1/e202000855. doi: 10.26508/lsa.202000855. Print 2021 Jan. PubMed |
| Loss of Furin in beta-Cells Induces an mTORC1-ATF4 Anabolic Pathway That Leads to beta-Cell Dysfunction. Brouwers B, Coppola I, Vints K, Dislich B, Jouvet N, Van Lommel L, Segers C, Gounko NV, Thorrez L, Schuit F, Lichtenthaler SF, Estall JL, Declercq J, Ramos-Molina B, Creemers JWM. Diabetes. 2021 Feb;70(2):492-503. doi: 10.2337/db20-0474. Epub 2020 Dec 4. PubMed |
| BAP1 deletion abrogates growth and metastasis of murine cutaneous melanoma. Luo X, Xu Y, Li Y, Zhang G, Huang S, Liu X, Ren Z, Liu S, Yu L. Melanoma Res. 2021 Apr 1;31(2):119-129. doi: 10.1097/CMR.0000000000000714. PubMed |
| Phosphorylation of PKCdelta by FER tips the balance from EGFR degradation to recycling. Lonic A, Gehling F, Belle L, Li X, Schieber NL, Nguyen EV, Goodall GJ, Parton RG, Daly RJ, Khew-Goodall Y. J Cell Biol. 2021 Feb 1;220(2). pii: 211661. doi: 10.1083/jcb.201902073. PubMed |
| Identification of Cathepsin D as a Plasma Biomarker for Alzheimer's Disease. Kim JW, Jung SY, Kim Y, Heo H, Hong CH, Seo SW, Choi SH, Son SJ, Lee S, Chang J. Cells. 2021 Jan 12;10(1). pii: cells10010138. doi: 10.3390/cells10010138. PubMed |
| Autophagy activation, lipotoxicity and lysosomal membrane permeabilization synergize to promote pimozide- and loperamide-induced glioma cell death. Meyer N, Henkel L, Linder B, Zielke S, Tascher G, Trautmann S, Geisslinger G, Munch C, Fulda S, Tegeder I, Kogel D. Autophagy. 2021 Nov;17(11):3424-3443. doi: 10.1080/15548627.2021.1874208. Epub 2021 Jan 19. PubMed |
| Resveratrol Promotes HIV-1 Tat Accumulation via AKT/FOXO1 Signaling Axis and Potentiates Vorinostat to Antagonize HIV-1 Latency. Feng Z, Yang Z, Gao X, Xue Y, Wang X. Curr HIV Res. 2021;19(3):238-247. doi: 10.2174/1570162X19666210118151249. PubMed |
| Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion. Papa G, Mallery DL, Albecka A, Welch LG, Cattin-Ortola J, Luptak J, Paul D, McMahon HT, Goodfellow IG, Carter A, Munro S, James LC. PLoS Pathog. 2021 Jan 25;17(1):e1009246. doi: 10.1371/journal.ppat.1009246. eCollection 2021 Jan. PubMed |
| Suppression of the NTS-CPS1 regulatory axis by AFF1 in lung adenocarcinoma cells. Yue J, Dai Q, Hao S, Zhu S, Liu X, Tang Z, Li M, Fang H, Lin C, Luo Z. J Biol Chem. 2021 Jan-Jun;296:100319. doi: 10.1016/j.jbc.2021.100319. Epub 2021 Jan 22. PubMed |
| Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition. Recasens-Alvarez C, Alexandre C, Kirkpatrick J, Nojima H, Huels DJ, Snijders AP, Vincent JP. Nat Cell Biol. 2021 Feb;23(2):127-135. doi: 10.1038/s41556-020-00626-1. Epub 2021 Jan 25. PubMed |
| l-Isoaspartyl Methyltransferase Deficiency in Zebrafish Leads to Impaired Calcium Signaling in the Brain. Soliman R, Cordero-Maldonado ML, Martins TG, Moein M, Conrotte JF, Warmack RA, Skupin A, Crawford AD, Clarke SG, Linster CL. Front Genet. 2021 Jan 21;11:612343. doi: 10.3389/fgene.2020.612343. eCollection 2020. PubMed |
Defective AMPA-mediated synaptic transmission and morphology in human neurons with hemizygous SHANK3 deletion engrafted in mouse prefrontal cortex.
Chiola S, Napan KL, Wang Y, Lazarenko RM, Armstrong CJ, Cui J, Shcheglovitov A.
Mol Psychiatry. 2021 Feb 8. pii: 10.1038/s41380-021-01023-2. doi: 10.1038/s41380-021-01023-2.
PubMed
Associated Plasmids |
| Generation of iPSC line from MYH7 R403L mutation carrier with severe hypertrophic cardiomyopathy and isogenic CRISPR/Cas9 corrected control. Fontaine V, Duboscq-Bidot L, Jouve C, Hamlin M, Curjol A, Briand V, Janiak P, Hulot JS, Pruniaux-Harnist MP, Charron P, Villard E. Stem Cell Res. 2021 Apr;52:102245. doi: 10.1016/j.scr.2021.102245. Epub 2021 Feb 11. PubMed |
| Marker counter-selection via CRISPR/Cas9 co-targeting for efficient generation of genome edited avian cell lines and germ cells. Challagulla A, Shi S, Nair K, O'Neil TE, Morris KR, Wise TG, Cahill DM, Tizard ML, Doran TJ, Jenkins KA. Anim Biotechnol. 2021 Mar 2:1-11. doi: 10.1080/10495398.2021.1885428. PubMed |
| SH3BP4 promotes neuropilin-1 and alpha5-integrin endocytosis and is inhibited by Akt. Burckhardt CJ, Minna JD, Danuser G. Dev Cell. 2021 Apr 19;56(8):1164-1181.e12. doi: 10.1016/j.devcel.2021.03.009. Epub 2021 Mar 23. PubMed |
| Inherited glycosylphosphatidylinositol defects cause the rare Emm-negative blood phenotype and developmental disorders. Duval R, Nicolas G, Willemetz A, Murakami Y, Mikdar M, Vrignaud C, Megahed H, Cartron JP, Masson C, Wehbi S, Koehl B, Hully M, Siquier K, Chemlay N, Rotig A, Lyonnet S, Colin Y, Barcia G, Cantagrel V, Le Van Kim C, Hermine O, Kinoshita T, Peyrard T, Azouzi S. Blood. 2021 Jul 1;137(26):3660-3669. doi: 10.1182/blood.2020009810. PubMed |
| Upregulation of deubiquitinase USP7 by transcription factor FOXO6 promotes EC progression via targeting the JMJD3/CLU axis. Li N, Zhao Z, Liu P, Zheng Y, Cai S, Sun Y, Wang B. Mol Ther Oncolytics. 2020 Dec 25;20:583-595. doi: 10.1016/j.omto.2020.12.008. eCollection 2021 Mar 26. PubMed |
| Humanized skeletal muscle in MYF5/MYOD/MYF6-null pig embryos. Maeng G, Das S, Greising SM, Gong W, Singh BN, Kren S, Mickelson D, Skie E, Gafni O, Sorensen JR, Weaver CV, Garry DJ, Garry MG. Nat Biomed Eng. 2021 Aug;5(8):805-814. doi: 10.1038/s41551-021-00693-1. Epub 2021 Mar 29. PubMed |
| Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus. Tang X, Fu J, Chen Z, Luo L, Li DW, Liu Y. Stem Cell Res. 2021 May;53:102310. doi: 10.1016/j.scr.2021.102310. Epub 2021 Mar 24. PubMed |
An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters.
Hamar J, Kultz D.
Sci Rep. 2021 Apr 12;11(1):7854. doi: 10.1038/s41598-021-87068-3.
PubMed
Associated Plasmids |
| Optimized CRISPR/Cas9-mediated single nucleotide mutation in adherent cancer cell lines. Gao P, Dong X, Wang Y, Wei GH. STAR Protoc. 2021 Mar 31;2(2):100419. doi: 10.1016/j.xpro.2021.100419. eCollection 2021 Jun 18. PubMed |
| Colorectal Cancer Stem Cell States Uncovered by Simultaneous Single-Cell Analysis of Transcriptome and Telomeres. Wang H, Gong P, Chen T, Gao S, Wu Z, Wang X, Li J, Marjani SL, Costa J, Weissman SM, Qi F, Pan X, Liu L. Adv Sci (Weinh). 2021 Feb 8;8(8):2004320. doi: 10.1002/advs.202004320. eCollection 2021 Apr. PubMed |
| FLYWCH1, a Multi-Functional Zinc Finger Protein Contributes to the DNA Repair Pathway. Almozyan S, Coulton J, Babaei-Jadidi R, Nateri AS. Cells. 2021 Apr 13;10(4). pii: cells10040889. doi: 10.3390/cells10040889. PubMed |
| c-Abl kinase regulates cell proliferation and ionizing radiation-induced G2/M arrest via phosphorylation of FHL2. Wang GF, Niu X, Liu H, Dong Q, Yao Y, Wang D, Liu X, Cao C. FEBS Open Bio. 2021 Jun;11(6):1731-1738. doi: 10.1002/2211-5463.13177. Epub 2021 May 19. PubMed |
| Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains. Piette BL, Alerasool N, Lin ZY, Lacoste J, Lam MHY, Qian WW, Tran S, Larsen B, Campos E, Peng J, Gingras AC, Taipale M. Mol Cell. 2021 Jun 17;81(12):2549-2565.e8. doi: 10.1016/j.molcel.2021.04.012. Epub 2021 May 5. PubMed |
| LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy. Li G, Kryczek I, Nam J, Li X, Li S, Li J, Wei S, Grove S, Vatan L, Zhou J, Du W, Lin H, Wang T, Subramanian C, Moon JJ, Cieslik M, Cohen M, Zou W. Nat Cell Biol. 2021 May;23(5):526-537. doi: 10.1038/s41556-021-00672-3. Epub 2021 May 6. PubMed |
| WNK1 is an assembly factor for the human ER membrane protein complex. Pleiner T, Hazu M, Tomaleri GP, Januszyk K, Oania RS, Sweredoski MJ, Moradian A, Guna A, Voorhees RM. Mol Cell. 2021 Jul 1;81(13):2693-2704.e12. doi: 10.1016/j.molcel.2021.04.013. Epub 2021 May 7. PubMed |
| Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells. Marx N, Dhiman H, Schmieder V, Freire CM, Nguyen LN, Klanert G, Borth N. Metab Eng. 2021 Jul;66:268-282. doi: 10.1016/j.ymben.2021.04.014. Epub 2021 May 16. PubMed |
| Human DICER helicase domain recruits PKR and modulates its antiviral activity. Montavon TC, Baldaccini M, Lefevre M, Girardi E, Chane-Woon-Ming B, Messmer M, Hammann P, Chicher J, Pfeffer S. PLoS Pathog. 2021 May 13;17(5):e1009549. doi: 10.1371/journal.ppat.1009549. eCollection 2021 May. PubMed |
| Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation. Feng D, Wang J, Yang W, Li J, Lin X, Zha F, Wang X, Ma L, Choi NT, Mii Y, Takada S, Huen MSY, Guo Y, Zhang L, Gao B. Sci Adv. 2021 May 14;7(20). pii: 7/20/eabg2099. doi: 10.1126/sciadv.abg2099. Print 2021 May. PubMed |
| AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies suppresses activation of YAP in alveolar epithelial type 2 cells. Kook S, Wang P, Meng S, Jetter CS, Sucre JMS, Benjamin JT, Gokey JJ, Hanby HA, Jaume A, Goetzl L, Marks MS, Guttentag SH. Proc Natl Acad Sci U S A. 2021 May 18;118(20). pii: 2025208118. doi: 10.1073/pnas.2025208118. PubMed |
| Decreased YAP activity reduces proliferative ability in human induced pluripotent stem cell of duchenne muscular dystrophy derived cardiomyocytes. Yasutake H, Lee JK, Hashimoto A, Masuyama K, Li J, Kuramoto Y, Higo S, Hikoso S, Hidaka K, Naito AT, Miyagawa S, Sawa Y, Komuro I, Sakata Y. Sci Rep. 2021 May 14;11(1):10351. doi: 10.1038/s41598-021-89603-8. PubMed |
| Knockout of the CMP-Sialic Acid Transporter SLC35A1 in Human Cell Lines Increases Transduction Efficiency of Adeno-Associated Virus 9: Implications for Gene Therapy Potency Assays. Banning A, Zakrzewicz A, Chen X, Gray SJ, Tikkanen R. Cells. 2021 May 19;10(5). pii: cells10051259. doi: 10.3390/cells10051259. PubMed |
| The H3K36me2 methyltransferase NSD1 modulates H3K27ac at active enhancers to safeguard gene expression. Fang Y, Tang Y, Zhang Y, Pan Y, Jia J, Sun Z, Zeng W, Chen J, Yuan Y, Fang D. Nucleic Acids Res. 2021 Jun 21;49(11):6281-6295. doi: 10.1093/nar/gkab473. PubMed |
| Mammalian cells use the autophagy process to restrict avian influenza virus replication. Liu S, Mok BW, Deng S, Liu H, Wang P, Song W, Chen P, Huang X, Zheng M, Lau SY, Cremin CJ, Tam CY, Li B, Jiang L, Chen Y, Yuen KY, Chen H. Cell Rep. 2021 Jun 8;35(10):109213. doi: 10.1016/j.celrep.2021.109213. PubMed |
| Chromosome compartments on the inactive X guide TAD formation independently of transcription during X-reactivation. Bauer M, Vidal E, Zorita E, Uresin N, Pinter SF, Filion GJ, Payer B. Nat Commun. 2021 Jun 9;12(1):3499. doi: 10.1038/s41467-021-23610-1. PubMed |
| RNA m(6)A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability. Xiong F, Wang R, Lee JH, Li S, Chen SF, Liao Z, Hasani LA, Nguyen PT, Zhu X, Krakowiak J, Lee DF, Han L, Tsai KL, Liu Y, Li W. Cell Res. 2021 Aug;31(8):861-885. doi: 10.1038/s41422-021-00515-8. Epub 2021 Jun 9. PubMed |
| Truncating variants in the SHANK1 gene are associated with a spectrum of neurodevelopmental disorders. May HJ, Jeong J, Revah-Politi A, Cohen JS, Chassevent A, Baptista J, Baugh EH, Bier L, Bottani A, Carminho A Rodrigues MT, Conlon C, Fluss J, Guipponi M, Kim CA, Matsumoto N, Person R, Primiano M, Rankin J, Shinawi M, Smith-Hicks C, Telegrafi A, Toy S, Uchiyama Y, Aggarwal V, Goldstein DB, Roche KW, Anyane-Yeboa K. Genet Med. 2021 Jun 10. pii: 10.1038/s41436-021-01222-w. doi: 10.1038/s41436-021-01222-w. PubMed |
| Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1. Huang PS, Wen MH, Xie X, Xu A, Lee DF, Chen TY. Stem Cell Res. 2021 Jul;54:102415. doi: 10.1016/j.scr.2021.102415. Epub 2021 Jun 6. PubMed |
| CRISPR mediated targeting of DUX4 distal regulatory element represses DUX4 target genes dysregulated in Facioscapulohumeral muscular dystrophy. Das S, Chadwick BP. Sci Rep. 2021 Jun 15;11(1):12598. doi: 10.1038/s41598-021-92096-0. PubMed |
| Generation and validation of APOE knockout human iPSC-derived cerebral organoids. Martens YA, Xu S, Tait R, Li G, Zhao XC, Lu W, Liu CC, Kanekiyo T, Bu G, Zhao J. STAR Protoc. 2021 Jun 5;2(2):100571. doi: 10.1016/j.xpro.2021.100571. eCollection 2021 Jun 18. PubMed |
| GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer's disease. Xu P, Chang JC, Zhou X, Wang W, Bamkole M, Wong E, Bettayeb K, Jiang LL, Huang T, Luo W, Xu H, Nairn AC, Flajolet M, Ip NY, Li YM, Greengard P. J Exp Med. 2021 Aug 2;218(8). pii: 212404. doi: 10.1084/jem.20202446. Epub 2021 Jun 22. PubMed |
| Modeling Phenotypic Heterogeneity of Glycogen Storage Disease Type 1a Liver Disease in Mice by Somatic CRISPR/CRISPR-associated protein 9-Mediated Gene Editing. Rutten MGS, Derks TGJ, Huijkman NCA, Bos T, Kloosterhuis NJ, van de Kolk KCWA, Wolters JC, Koster MH, Bongiovanni L, Thomas RE, de Bruin A, van de Sluis B, Oosterveer MH. Hepatology. 2021 Nov;74(5):2491-2507. doi: 10.1002/hep.32022. Epub 2021 Aug 15. PubMed |
| Inhibiting PHGDH with NCT-503 reroutes glucose-derived carbons into the TCA cycle, independently of its on-target effect. Arlt B, Mastrobuoni G, Wuenschel J, Astrahantseff K, Eggert A, Kempa S, Deubzer HE. J Enzyme Inhib Med Chem. 2021 Dec;36(1):1282-1289. doi: 10.1080/14756366.2021.1935917. PubMed |
| Generation of human embryonic stem cell models to exploit the EWSR1-CREB fusion promiscuity as a common pathway of transformation in human tumors. Vanoli F, Meskauskaite B, Herviou L, Mallen W, Sung YS, Fujisawa Y, Zhang L, Simon S, Huangfu D, Jasin M, Antonescu CR. Oncogene. 2021 Aug;40(32):5095-5104. doi: 10.1038/s41388-021-01843-0. Epub 2021 Jun 30. PubMed |
| P2X7 Receptors and TMEM16 Channels Are Functionally Coupled with Implications for Macropore Formation and Current Facilitation. Dunning K, Martz A, Peralta FA, Cevoli F, Boue-Grabot E, Compan V, Gautherat F, Wolf P, Chataigneau T, Grutter T. Int J Mol Sci. 2021 Jun 18;22(12). pii: ijms22126542. doi: 10.3390/ijms22126542. PubMed |
| Targeting of SET/I2PP2A oncoprotein inhibits Gli1 transcription revealing a new modulator of Hedgehog signaling. Serifi I, Besta S, Karetsou Z, Giardoglou P, Beis D, Niewiadomski P, Papamarcaki T. Sci Rep. 2021 Jul 6;11(1):13940. doi: 10.1038/s41598-021-93440-0. PubMed |
| JunD, not c-Jun, is the AP-1 transcription factor required for Ras-induced lung cancer. Ruiz EJ, Lan L, Diefenbacher ME, Riising EM, Da Costa C, Chakraborty A, Hoeck JD, Spencer-Dene B, Kelly G, David JP, Nye E, Downward J, Behrens A. JCI Insight. 2021 Jul 8;6(13). pii: e124985. doi: 10.1172/jci.insight.124985. PubMed |
| Meiotic recombination mirrors patterns of germline replication in mice and humans. Pratto F, Brick K, Cheng G, Lam KG, Cloutier JM, Dahiya D, Wellard SR, Jordan PW, Camerini-Otero RD. Cell. 2021 Aug 5;184(16):4251-4267.e20. doi: 10.1016/j.cell.2021.06.025. Epub 2021 Jul 13. PubMed |
| Repurposed floxacins targeting RSK4 prevent chemoresistance and metastasis in lung and bladder cancer. Chrysostomou S, Roy R, Prischi F, Thamlikitkul L, Chapman KL, Mufti U, Peach R, Ding L, Hancock D, Moore C, Molina-Arcas M, Mauri F, Pinato DJ, Abrahams JM, Ottaviani S, Castellano L, Giamas G, Pascoe J, Moonamale D, Pirrie S, Gaunt C, Billingham L, Steven NM, Cullen M, Hrouda D, Winkler M, Post J, Cohen P, Salpeter SJ, Bar V, Zundelevich A, Golan S, Leibovici D, Lara R, Klug DR, Yaliraki SN, Barahona M, Wang Y, Downward J, Skehel JM, Ali MMU, Seckl MJ, Pardo OE. Sci Transl Med. 2021 Jul 14;13(602). pii: 13/602/eaba4627. doi: 10.1126/scitranslmed.aba4627. PubMed |
| Genetic screens identify a context-specific PI3K/p27Kip1 node driving extrahepatic biliary cancer. Falcomata C, Barthel S, Ulrich A, Diersch S, Veltkamp C, Rad L, Boniolo F, Solar M, Steiger K, Seidler B, Zukowska M, Madej J, Wang M, Ollinger R, Maresch R, Barenboim M, Eser S, Tschurtschenthaler M, Mehrabi A, Roessler S, Goeppert B, Kind A, Schnieke A, Robles MS, Bradley A, Schmid RM, Schmidt-Supprian M, Reichert M, Weichert W, Sansom OJ, Morton JP, Rad R, Schneider G, Saur D. Cancer Discov. 2021 Jul 19. pii: 2159-8290.CD-21-0209. doi: 10.1158/2159-8290.CD-21-0209. PubMed |
| A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation. Zhang W, Yang X, Chen L, Liu YY, Venkatarangan V, Reist L, Hanson P, Xu H, Wang Y, Li M. PLoS Biol. 2021 Jul 23;19(7):e3001361. doi: 10.1371/journal.pbio.3001361. eCollection 2021 Jul. PubMed |
| Kaiso Regulates DNA Methylation Homeostasis. Kaplun D, Starshin A, Sharko F, Gainova K, Filonova G, Zhigalova N, Mazur A, Prokhortchouk E, Zhenilo S. Int J Mol Sci. 2021 Jul 15;22(14). pii: ijms22147587. doi: 10.3390/ijms22147587. PubMed |
| Comparison of Ciliary Targeting of Two Rhodopsin-Like GPCRs: Role of C-Terminal Localization Sequences in Relation to Cilium Type. Chadha A, Paniagua AE, Williams DS. J Neurosci. 2021 Sep 8;41(36):7514-7531. doi: 10.1523/JNEUROSCI.0357-21.2021. Epub 2021 Jul 22. PubMed |
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| SNX27-driven membrane localisation of OTULIN antagonises linear ubiquitination and NF-kappaB signalling activation. Shi R, Shi X, Qin D, Tang S, Vermeulen M, Zhang X. Cell Biosci. 2021 Jul 27;11(1):146. doi: 10.1186/s13578-021-00659-5. PubMed |
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| Establishment of an Efficient Immortalization Strategy Using HMEJ-Based bTERT Insertion for Bovine Cells. Zhang Z, Han Z, Guo Y, Liu X, Gao Y, Zhang Y. Int J Mol Sci. 2021 Nov 21;22(22). pii: ijms222212540. doi: 10.3390/ijms222212540. PubMed |
| The activity of glyoxylase 1 is regulated by glucose-responsive phosphorylation on Tyr136. Cortizo FG, Pfaff D, Wirth A, Schlotterer A, Medert R, Morgenstern J, Weber T, Hammes HP, Fleming T, Nawroth PP, Freichel M, Teleman AA. Mol Metab. 2022 Jan;55:101406. doi: 10.1016/j.molmet.2021.101406. Epub 2021 Nov 25. PubMed |
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| Novel Quinic Acid Glycerates from Tussilago farfara Inhibit Polypeptide GalNAc-Transferase. Feng J, Li YP, Hu Y, Zhou Y, Zhang H, Wu F. Chembiochem. 2022 Feb 4;23(3):e202100539. doi: 10.1002/cbic.202100539. Epub 2021 Dec 9. PubMed |
| Large Multicohort Study Reveals a Prostate Cancer Susceptibility Allele at 5p15 Regulating TERT via Androgen Signaling-Orchestrated Chromatin Binding of E2F1 and MYC. Dong X, Zhang Q, Hao J, Xie Q, Xu B, Zhang P, Lu H, Huang Q, Yang T, Wei GH, Na R, Gao P. Front Oncol. 2021 Nov 10;11:754206. doi: 10.3389/fonc.2021.754206. eCollection 2021. PubMed |
| CRISPR-targeted genome editing of human induced pluripotent stem cell-derived hepatocytes for the treatment of Wilson's disease. Wei R, Yang J, Cheng CW, Ho WI, Li N, Hu Y, Hong X, Fu J, Yang B, Liu Y, Jiang L, Lai WH, Au KW, Tsang WL, Tse YL, Ng KM, Esteban MA, Tse HF. JHEP Rep. 2021 Oct 30;4(1):100389. doi: 10.1016/j.jhepr.2021.100389. eCollection 2022 Jan. PubMed |
| Splice-Variant Knock-Out of TGFbeta Receptors Perturbates the Proteome of Ovarian Carcinoma Cells. Jacobs Catane L, Moshel O, Smith Y, Davidson B, Reich R. Int J Mol Sci. 2021 Nov 23;22(23). pii: ijms222312647. doi: 10.3390/ijms222312647. PubMed |
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| MITOL regulates phosphatidic acid-binding activity of RMDN3/PTPIP51. Ito N, Takahashi T, Shiiba I, Nagashima S, Inatome R, Yanagi S. J Biochem. 2021 Dec 29. pii: 6488375. doi: 10.1093/jb/mvab153. PubMed |
| Collagen VI Regulates Motor Circuit Plasticity and Motor Performance by Cannabinoid Modulation. Lam DD, Williams RH, Lujan E, Tanabe K, Huber G, Saw NL, Merl-Pham J, Salminen AV, Lohse D, Spendiff S, Plastini MJ, Zech M, Lochmuller H, Geerlof A, Hauck SM, Shamloo M, Wernig M, Winkelmann J. J Neurosci. 2022 Feb 23;42(8):1557-1573. doi: 10.1523/JNEUROSCI.0962-21.2021. Epub 2021 Dec 27. PubMed |
| Genome Editing with AAV-BR1-CRISPR in Postnatal Mouse Brain Endothelial Cells. Song X, Cui Y, Wang Y, Zhang Y, He Q, Yu Z, Xu C, Ning H, Han Y, Cai Y, Cheng X, Wang J, Teng Y, Yang X, Wang J. Int J Biol Sci. 2022 Jan 1;18(2):652-660. doi: 10.7150/ijbs.64188. eCollection 2022. PubMed |
| CRISPR/Cas9 Genome Editing vs. Over-Expression for Fluorescent Extracellular Vesicle-Labeling: A Quantitative Analysis. Strohmeier K, Hofmann M, Hauser F, Sivun D, Puthukodan S, Karner A, Sandner G, Le Renard PE, Jacak J, Mairhofer M. Int J Mol Sci. 2021 Dec 28;23(1):282. doi: 10.3390/ijms23010282. PubMed |
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| The Establishment of Quantitatively Regulating Expression Cassette with sgRNA Targeting BIRC5 to Elucidate the Synergistic Pathway of Survivin with P-Glycoprotein in Cancer Multi-Drug Resistance. Deng C, Hu F, Zhao Z, Zhou Y, Liu Y, Zhang T, Li S, Zheng W, Zhang W, Wang T, Ma X. Front Cell Dev Biol. 2022 Jan 3;9:797005. doi: 10.3389/fcell.2021.797005. eCollection 2021. PubMed |
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Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage.
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Associated Plasmids |
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| CRISPR/Cas9-Mediated Disruption of ZNF543 Gene: An Approach Toward Discovering Its Relation to TRIM28 Gene in Parkinson's Disease. Hashemabadi M, Sasan H, Amandadi M, Esmaeilzadeh-Salestani K, Esmaeili-Mahani S, Ravan H. Mol Biotechnol. 2022 Apr 25. pii: 10.1007/s12033-022-00494-0. doi: 10.1007/s12033-022-00494-0. PubMed |
| Ebola virus VP35 hijacks the PKA-CREB1 pathway for replication and pathogenesis by AKIP1 association. Zhu L, Gao T, Huang Y, Jin J, Wang D, Zhang L, Jin Y, Li P, Hu Y, Wu Y, Liu H, Dong Q, Wang G, Zheng T, Song C, Bai Y, Zhang X, Liu Y, Yang W, Xu K, Zou G, Zhao L, Cao R, Zhong W, Xia X, Xiao G, Liu X, Cao C. Nat Commun. 2022 Apr 26;13(1):2256. doi: 10.1038/s41467-022-29948-4. PubMed |
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| Rapid acceleration of KRAS-mutant pancreatic carcinogenesis via remodeling of tumor immune microenvironment by PPARdelta. Liu Y, Deguchi Y, Wei D, Liu F, Moussalli MJ, Deguchi E, Li D, Wang H, Valentin LA, Colby JK, Wang J, Zheng X, Ying H, Gagea M, Ji B, Shi J, Yao JC, Zuo X, Shureiqi I. Nat Commun. 2022 May 13;13(1):2665. doi: 10.1038/s41467-022-30392-7. PubMed |
| The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling. Schembs L, Willems A, Hasenpusch-Theil K, Cooper JD, Whiting K, Burr K, Bostrand SMK, Selvaraj BT, Chandran S, Theil T. Cell Rep. 2022 May 17;39(7):110811. doi: 10.1016/j.celrep.2022.110811. PubMed |
| Controlled X-chromosome dynamics defines meiotic potential of female mouse in vitro germ cells. Severino J, Bauer M, Mattimoe T, Arecco N, Cozzuto L, Lorden P, Hamada N, Nosaka Y, Nagaoka SI, Audergon P, Tarruell A, Heyn H, Hayashi K, Saitou M, Payer B. EMBO J. 2022 Jun 14;41(12):e109457. doi: 10.15252/embj.2021109457. Epub 2022 May 23. PubMed |
| SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication. Liu H, Bai Y, Zhang X, Gao T, Liu Y, Li E, Wang X, Cao Z, Zhu L, Dong Q, Hu Y, Wang G, Song C, Niu X, Zheng T, Wang D, Liu Z, Jin Y, Li P, Bian X, Cao C, Liu X. J Virol. 2022 Jun 22;96(12):e0041222. doi: 10.1128/jvi.00412-22. Epub 2022 Jun 2. PubMed |
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| Heparan sulfate dependent binding of plasmatic von Willebrand factor to blood circulating melanoma cells attenuates metastasis. Wang Y, Liu X, Obser T, Bauer AT, Heyes M, Starzonek S, Zulal M, Opitz K, Ott L, Riethdorf S, Lange T, Pantel K, Bendas G, Schneider SW, Kusche-Gullberg M, Gorzelanny C. Matrix Biol. 2022 Aug;111:76-94. doi: 10.1016/j.matbio.2022.06.002. Epub 2022 Jun 9. PubMed |
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| CD95 gene deletion may reduce clonogenic growth and invasiveness of human glioblastoma cells in a CD95 ligand-independent manner. Quijano-Rubio C, Silginer M, Weller M. Cell Death Discov. 2022 Jul 29;8(1):341. doi: 10.1038/s41420-022-01133-y. PubMed |
| A self-amplifying USP14-TAZ loop drives the progression and liver metastasis of pancreatic ductal adenocarcinoma. Zhao C, Gong J, Bai Y, Yin T, Zhou M, Pan S, Liu Y, Gao Y, Zhang Z, Shi Y, Zhu F, Zhang H, Wang M, Qin R. Cell Death Differ. 2023 Jan;30(1):1-15. doi: 10.1038/s41418-022-01040-w. Epub 2022 Jul 29. PubMed |
| Enhanced phosphorylation of c-Jun by cisplatin treatment as a potential predictive biomarker for cisplatin response in combination with patient-derived tumor organoids. Tsukamoto Y, Kurogi S, Shibata T, Suzuki K, Hirashita Y, Fumoto S, Yano S, Yanagihara K, Nakada C, Mieno F, Kinoshita K, Fuchino T, Mizukami K, Ueda Y, Etoh T, Uchida T, Hanada T, Takekawa M, Daa T, Shirao K, Hironaka S, Murakami K, Inomata M, Hijiya N, Moriyama M. Lab Invest. 2022 Dec;102(12):1355-1366. doi: 10.1038/s41374-022-00827-2. Epub 2022 Aug 3. PubMed |
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