Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage.
Bollen Y, Hageman JH, van Leenen P, Derks LLM, Ponsioen B, Buissant des Amorie JR, Verlaan-Klink I, van den Bos M, Terstappen LWMM, van Boxtel R, Snippert HJG
PLoS Biol. 2022 Jan 28;20(1):e3001527. doi: 10.1371/journal.pbio.3001527. PubMed Article
Bollen Y, Hageman JH, van Leenen P, Derks LLM, Ponsioen B, Buissant des Amorie JR, Verlaan-Klink I, van den Bos M, Terstappen LWMM, van Boxtel R, Snippert HJG
PLoS Biol. 2022 Jan 28;20(1):e3001527. doi: 10.1371/journal.pbio.3001527. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169216 | TVBB C-term-Dendra2 | Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus |
| 169217 | TVBB C-term-Dendra2-Puro | Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus |
| 169218 | TVBB N-term-mScarlet | Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus |
| 169219 | TVBB C-term-mScarlet | Targeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locus |
| 169220 | TVBB C-term-mScarlet-Puro | Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus |
| 169221 | TVBB C-term-mScarlet-Blast | Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus |
| 169222 | TVBB N-term-mTurquoise2 | Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus |
| 169223 | TVBB C-term-mTurquoise2 | Targeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locus |
| 169224 | TVBB C-term-mTurquoise2-Puro | Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus |
| 169225 | TVBB C-term-mTurquoise2-Hygro | Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus |
| 169226 | TVBB N-term-mNeongreen | Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus |
| 169227 | TVBB C-term-mNeongreen | Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus |
| 169228 | TVBB C-term-mNeongreen-Puro | Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus |
| 169229 | TVBB C-term-mNeongreen-Blast | Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus |