Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.
Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, Lin SC
Nature. 2017 Aug 3;548(7665):112-116. doi: 10.1038/nature23275. Epub 2017 Jul 19. PubMed Article
Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, Lin SC
Nature. 2017 Aug 3;548(7665):112-116. doi: 10.1038/nature23275. Epub 2017 Jul 19. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210806 | pBoBi-hALDOA | For stably expression in cells |
| 210807 | pBoBi-hALDOA D34S | To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P) |
| 210808 | pBoBi-hALDOA K230A | To block the formation of the initial Schiff base with FBP |
| 210809 | pcDNA3.3-hALDOA | For transient transfection |
| 210810 | pcDNA3.3-hALDOA D34S | To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P) |
| 210811 | pcDNA3.3-hALDOA K230A | To block the formation of the initial Schiff base with FBP |
| 210812 | pAdEasy-hALDOA | To package adenovirus expressing wild-type aldolaseA |
| 210813 | pAdEasy-hALDOA D34S | To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P) |