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pCW-Cas9 Citations (91)

Plasmid Article: Genetic screens in human cells using the CRISPR-Cas9 system.
Wang T, Wei JJ, Sabatini DM, Lander ES Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12.
PubMed Journal

Articles Citing pCW-Cas9

Efficient Gene Disruption in Cultured Primary Human Endothelial Cells by CRISPR/Cas9. Abrahimi P, Chang WG, Kluger MS, Qyang Y, Tellides G, Saltzman WM, Pober JS. Circ Res. 2015 Jul 3;117(2):121-8. doi: 10.1161/CIRCRESAHA.117.306290. Epub 2015 May 4. PubMed
miR-663 Suppresses Oncogenic Function of CXCR4 in Glioblastoma. Shi Y, Chen C, Yu SZ, Liu Q, Rao J, Zhang HR, Xiao HL, Fu TW, Long H, He ZC, Zhou K, Yao XH, Cui YH, Zhang X, Ping YF, Bian XW. Clin Cancer Res. 2015 May 28. PubMed
The C-terminal Domain (CTD) of Human DNA Glycosylase NEIL1 Is Required for Forming BERosome Repair Complex with DNA Replication Proteins at the Replicating Genome: Dominant Negative Function of CTD. Hegde PM, Dutta A, Sengupta S, Mitra J, Adhikari S, Tomkinson AE, Li GM, Boldogh I, Hazra TK, Mitra S, Hegde ML. J Biol Chem. 2015 Jul 1. pii: jbc.M115.642918. PubMed
CRISPR/Cas9 System as an Agent for Eliminating Polyomavirus JC Infection. Wollebo HS, Bellizzi A, Kaminski R, Hu W, White MK, Khalili K. PLoS One. 2015 Sep 11;10(9):e0136046. doi: 10.1371/journal.pone.0136046. eCollection 2015. PubMed
AMPK Protects Leukemia-Initiating Cells in Myeloid Leukemias from Metabolic Stress in the Bone Marrow. Saito Y, Chapple RH, Lin A, Kitano A, Nakada D. Cell Stem Cell. 2015 Sep 29. pii: S1934-5909(15)00374-4. doi: 10.1016/j.stem.2015.08.019. PubMed

Associated Plasmids

Genome editing through large insertion leads to the skipping of targeted exon. Uddin B, Chen NP, Panic M, Schiebel E. BMC Genomics. 2015 Dec 21;16(1):1082. doi: 10.1186/s12864-015-2284-8. PubMed
Fan1 deficiency results in DNA interstrand cross-link repair defects, enhanced tissue karyomegaly, and organ dysfunction. Thongthip S, Bellani M, Gregg SQ, Sridhar S, Conti BA, Chen Y, Seidman MM, Smogorzewska A. Genes Dev. 2016 Mar 15;30(6):645-59. doi: 10.1101/gad.276261.115. PubMed
BIN1 regulates BACE1 intracellular trafficking and amyloid-beta production. Miyagawa T, Ebinuma I, Morohashi Y, Hori Y, Young Chang M, Hattori H, Maehara T, Yokoshima S, Fukuyama T, Tsuji S, Iwatsubo T, Prendergast GC, Tomita T. Hum Mol Genet. 2016 Jul 15;25(14):2948-2958. Epub 2016 May 14. PubMed
Focal Adhesion Kinase regulates the DNA damage response and its inhibition radiosensitizes mutant KRAS lung cancer. Tang KJ, Constanzo J, Venkateswaran N, Melegari M, Ilcheva M, Morales JC, Skoulidis F, Heymach JV, Boothman DA, Scaglioni PP. Clin Cancer Res. 2016 May 24. pii: clincanres.2603.2015. PubMed
Generation of porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene by somatic cell nuclear transfer. Liu G, Liu K, Wei H, Li L, Zhang S. Mol Med Rep. 2016 Sep;14(3):2527-33. doi: 10.3892/mmr.2016.5530. Epub 2016 Jul 19. PubMed
Hypersensitivity to DNA damage in antephase as a safeguard for genome stability. Feringa FM, Krenning L, Koch A, van den Berg J, van den Broek B, Jalink K, Medema RH. Nat Commun. 2016 Aug 26;7:12618. doi: 10.1038/ncomms12618. PubMed
A PGC1alpha-mediated transcriptional axis suppresses melanoma metastasis. Luo C, Lim JH, Lee Y, Granter SR, Thomas A, Vazquez F, Widlund HR, Puigserver P. Nature. 2016 Sep 15;537(7620):422-426. doi: 10.1038/nature19347. Epub 2016 Aug 31. PubMed
Systematic Analysis of Known and Candidate Lysine Demethylases in the Regulation of Myoblast Differentiation. Munehira Y, Yang Z, Gozani O. J Mol Biol. 2016 Oct 11. pii: S0022-2836(16)30419-3. doi: 10.1016/j.jmb.2016.10.004. PubMed
A pH- and ionic strength-dependent conformational change in the neck region regulates DNGR-1 function in dendritic cells. Hanc P, Schulz O, Fischbach H, Martin SR, Kjaer S, Reis E Sousa C. EMBO J. 2016 Nov 15;35(22):2484-2497. Epub 2016 Oct 17. PubMed
Deficiency of XLF and PAXX prevents DNA double-strand break repair by non-homologous end joining in lymphocytes. Hung PJ, Chen BR, George R, Liberman C, Morales AJ, Colon-Ortiz P, Tyler JK, Sleckman BP, Bredemeyer AL. Cell Cycle. 2017 Feb;16(3):286-295. doi: 10.1080/15384101.2016.1253640. Epub 2016 Nov 10. PubMed
A High-Throughput Strategy for Dissecting Mammalian Genetic Interactions. Stockman VB, Ghamsari L, Lasso G, Honig B, Shapira SD, Wang HH. PLoS One. 2016 Dec 9;11(12):e0167617. doi: 10.1371/journal.pone.0167617. eCollection 2016. PubMed
Mechanism of PKR Inhibition by Human Cytomegalovirus pTRS1. Vincent HA, Ziehr B, Moorman NJ. J Virol. 2016 Dec 14. pii: JVI.01574-16. PubMed
MicroRNA-202 maintains spermatogonial stem cells by inhibiting cell cycle regulators and RNA binding proteins. Chen J, Cai T, Zheng C, Lin X, Wang G, Liao S, Wang X, Gan H, Zhang D, Hu X, Wang S, Li Z, Feng Y, Yang F, Han C. Nucleic Acids Res. 2016 Dec 19. pii: gkw1287. doi: 10.1093/nar/gkw1287. PubMed
Application of CRISPR/Cas9 to Autophagy Research. O'Prey J, Sakamaki J, Baudot AD, New M, Van Acker T, Tooze SA, Long JS, Ryan KM. Methods Enzymol. 2017;588:79-108. doi: 10.1016/bs.mie.2016.09.076. Epub 2016 Dec 1. PubMed
ENL links histone acetylation to oncogenic gene expression in acute myeloid leukaemia. Wan L, Wen H, Li Y, Lyu J, Xi Y, Hoshii T, Joseph JK, Wang X, Loh YE, Erb MA, Souza AL, Bradner JE, Shen L, Li W, Li H, Allis CD, Armstrong SA, Shi X. Nature. 2017 Mar 9;543(7644):265-269. doi: 10.1038/nature21687. Epub 2017 Mar 1. PubMed
CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Xia L, Huang W, Bellani M, Seidman MM, Wu K, Fan D, Nie Y, Cai Y, Zhang YW, Yu LR, Li H, Zahnow CA, Xie W, Chiu Yen RW, Rassool FV, Baylin SB. Cancer Cell. 2017 May 8;31(5):653-668.e7. doi: 10.1016/j.ccell.2017.04.005. PubMed
MLL2, Not MLL1, Plays a Major Role in Sustaining MLL-Rearranged Acute Myeloid Leukemia. Chen Y, Anastassiadis K, Kranz A, Stewart AF, Arndt K, Waskow C, Yokoyama A, Jones K, Neff T, Lee Y, Ernst P. Cancer Cell. 2017 Jun 12;31(6):755-770.e6. doi: 10.1016/j.ccell.2017.05.002. PubMed
Chromatin-Bound Oxidized alpha-Synuclein Causes Strand Breaks in Neuronal Genomes in in vitro Models of Parkinson's Disease. Vasquez V, Mitra J, Hegde PM, Pandey A, Sengupta S, Mitra S, Rao KS, Hegde ML. J Alzheimers Dis. 2017;60(s1):S133-S150. doi: 10.3233/JAD-170342. PubMed
Smac mimetics and oncolytic viruses synergize in driving anticancer T-cell responses through complementary mechanisms. Kim DS, Dastidar H, Zhang C, Zemp FJ, Lau K, Ernst M, Rakic A, Sikdar S, Rajwani J, Naumenko V, Balce DR, Ewanchuk BW, Tailor P, Yates RM, Jenne C, Gafuik C, Mahoney DJ. Nat Commun. 2017 Aug 24;8(1):344. doi: 10.1038/s41467-017-00324-x. PubMed
RUNX2 expression in thyroid and breast cancer requires the cooperation of three non-redundant enhancers under the control of BRD4 and c-JUN. Sancisi V, Manzotti G, Gugnoni M, Rossi T, Gandolfi G, Gobbi G, Torricelli F, Catellani F, Faria do Valle I, Remondini D, Castellani G, Ragazzi M, Piana S, Ciarrocchi A. Nucleic Acids Res. 2017 Nov 2;45(19):11249-11267. doi: 10.1093/nar/gkx802. PubMed
CRISPR-Cas9-mediated functional dissection of 3'-UTRs. Zhao W, Siegel D, Biton A, Tonqueze OL, Zaitlen N, Ahituv N, Erle DJ. Nucleic Acids Res. 2017 Oct 13;45(18):10800-10810. doi: 10.1093/nar/gkx675. PubMed
Ebola virus requires phosphatidylinositol (3,5) bisphosphate production for efficient viral entry. Qiu S, Leung A, Bo Y, Kozak RA, Anand SP, Warkentin C, Salambanga FDR, Cui J, Kobinger G, Kobasa D, Cote M. Virology. 2018 Jan 1;513:17-28. doi: 10.1016/j.virol.2017.09.028. Epub 2017 Oct 12. PubMed
Antagonism of the Protein Kinase R Pathway in Human Cells by Rhesus Cytomegalovirus. Child SJ, Hickson SE, Bayer A, Malouli D, Fruh K, Geballe AP. J Virol. 2018 Feb 26;92(6). pii: JVI.01793-17. doi: 10.1128/JVI.01793-17. Print 2018 Mar 15. PubMed
On the design of CRISPR-based single-cell molecular screens. Hill AJ, McFaline-Figueroa JL, Starita LM, Gasperini MJ, Matreyek KA, Packer J, Jackson D, Shendure J, Trapnell C. Nat Methods. 2018 Feb 19. pii: nmeth.4604. doi: 10.1038/nmeth.4604. PubMed

Associated Plasmids

A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Hoshii T, Cifani P, Feng Z, Huang CH, Koche R, Chen CW, Delaney CD, Lowe SW, Kentsis A, Armstrong SA. Cell. 2018 Feb 22;172(5):1007-1021.e17. doi: 10.1016/j.cell.2018.01.032. PubMed
CAMKs support development of acute myeloid leukemia. Kang X, Cui C, Wang C, Wu G, Chen H, Lu Z, Chen X, Wang L, Huang J, Geng H, Zhao M, Chen Z, Muschen M, Wang HY, Zhang CC. J Hematol Oncol. 2018 Feb 27;11(1):30. doi: 10.1186/s13045-018-0574-8. PubMed
MicroRNA-focused CRISPR-Cas9 Library Screen Reveals Fitness-Associated miRNAs. Kurata JS, Lin RJ. RNA. 2018 May 2. pii: rna.066282.118. doi: 10.1261/rna.066282.118. PubMed

Associated Plasmids

Oncogenic Properties of NEAT1 in Prostate Cancer Cells Depend on the CDC5L-AGRN Transcriptional Regulation Circuit. Li X, Wang X, Song W, Xu H, Huang R, Wang Y, Zhao W, Xiao Z, Yang X. Cancer Res. 2018 Aug 1;78(15):4138-4149. doi: 10.1158/0008-5472.CAN-18-0688. Epub 2018 Jun 5. PubMed
Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1. Magliozzi R, Carrero ZI, Low TY, Yuniati L, Valdes-Quezada C, Kruiswijk F, van Wijk K, Heck AJR, Jackson CL, Guardavaccaro D. Cell Rep. 2018 Jun 12;23(11):3381-3391.e4. doi: 10.1016/j.celrep.2018.05.031. PubMed
A multiprotein supercomplex controlling oncogenic signalling in lymphoma. Phelan JD, Young RM, Webster DE, Roulland S, Wright GW, Kasbekar M, Shaffer AL 3rd, Ceribelli M, Wang JQ, Schmitz R, Nakagawa M, Bachy E, Huang DW, Ji Y, Chen L, Yang Y, Zhao H, Yu X, Xu W, Palisoc MM, Valadez RR, Davies-Hill T, Wilson WH, Chan WC, Jaffe ES, Gascoyne RD, Campo E, Rosenwald A, Ott G, Delabie J, Rimsza LM, Rodriguez FJ, Estephan F, Holdhoff M, Kruhlak MJ, Hewitt SM, Thomas CJ, Pittaluga S, Oellerich T, Staudt LM. Nature. 2018 Aug;560(7718):387-391. doi: 10.1038/s41586-018-0290-0. Epub 2018 Jun 20. PubMed
CK1alpha and IRF4 are essential and independent effectors of immunomodulatory drugs in primary effusion lymphoma. Patil A, Manzano M, Gottwein E. Blood. 2018 Aug 9;132(6):577-586. doi: 10.1182/blood-2018-01-828418. Epub 2018 Jun 28. PubMed

Associated Plasmids

MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining. Hung PJ, Johnson B, Chen BR, Byrum AK, Bredemeyer AL, Yewdell WT, Johnson TE, Lee BJ, Deivasigamani S, Hindi I, Amatya P, Gross ML, Paull TT, Pisapia DJ, Chaudhuri J, Petrini JJH, Mosammaparast N, Amarasinghe GK, Zha S, Tyler JK, Sleckman BP. Mol Cell. 2018 Jul 19;71(2):332-342.e8. doi: 10.1016/j.molcel.2018.06.018. Epub 2018 Jul 12. PubMed
MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD. Cichewicz MA, Kiran M, Przanowska RK, Sobierajska E, Shibata Y, Dutta A. Mol Cell Biol. 2018 Sep 28;38(20). pii: MCB.00655-17. doi: 10.1128/MCB.00655-17. Print 2018 Oct 15. PubMed
PARP2 mediates branched poly ADP-ribosylation in response to DNA damage. Chen Q, Kassab MA, Dantzer F, Yu X. Nat Commun. 2018 Aug 13;9(1):3233. doi: 10.1038/s41467-018-05588-5. PubMed
Function of HNRNPC in breast cancer cells by controlling the dsRNA-induced interferon response. Wu Y, Zhao W, Liu Y, Tan X, Li X, Zou Q, Xiao Z, Xu H, Wang Y, Yang X. EMBO J. 2018 Dec 3;37(23). pii: embj.201899017. doi: 10.15252/embj.201899017. Epub 2018 Aug 29. PubMed
A limited number of double-strand DNA breaks is sufficient to delay cell cycle progression. van den Berg J, G Manjon A, Kielbassa K, Feringa FM, Freire R, Medema RH. Nucleic Acids Res. 2018 Nov 2;46(19):10132-10144. doi: 10.1093/nar/gky786. PubMed
PLK1 targets CtIP to promote microhomology-mediated end joining. Wang H, Qiu Z, Liu B, Wu Y, Ren J, Liu Y, Zhao Y, Wang Y, Hao S, Li Z, Peng B, Xu X. Nucleic Acids Res. 2018 Nov 16;46(20):10724-10739. doi: 10.1093/nar/gky810. PubMed
Unscheduled HDAC4 repressive activity in human fibroblasts triggers TP53-dependent senescence and favors cell transformation. Paluvai H, Di Giorgio E, Brancolini C. Mol Oncol. 2018 Dec;12(12):2165-2181. doi: 10.1002/1878-0261.12392. Epub 2018 Nov 14. PubMed
An Inducible Alpha-Synuclein Expressing Neuronal Cell Line Model for Parkinson's Disease1. Vasquez V, Mitra J, Perry G, Rao KS, Hegde ML. J Alzheimers Dis. 2018;66(2):453-460. doi: 10.3233/JAD-180610. PubMed
SHQ1 regulation of RNA splicing is required for T-lymphoblastic leukemia cell survival. Su H, Hu J, Huang L, Yang Y, Thenoz M, Kuchmiy A, Hu Y, Li P, Feng H, Zhou Y, Taghon T, Van Vlierberghe P, Qing G, Chen Z, Liu H. Nat Commun. 2018 Oct 15;9(1):4281. doi: 10.1038/s41467-018-06523-4. PubMed
LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, He L, Chen Y, Chen H, Luo W, Lu Z, Xie J, Churchill H, Xu Y, Zhou Z, Wu G, Yu C, John S, Hirayasu K, Nguyen N, Liu X, Huang F, Li L, Deng H, Tang H, Sadek AH, Zhang L, Huang T, Zou Y, Chen B, Zhu H, Arase H, Xia N, Jiang Y, Collins R, You MJ, Homsi J, Unni N, Lewis C, Chen GQ, Fu YX, Liao XC, An Z, Zheng J, Zhang N, Zhang CC. Nature. 2018 Oct;562(7728):605-609. doi: 10.1038/s41586-018-0615-z. Epub 2018 Oct 17. PubMed
Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors. Bulatov E, Sayarova R, Mingaleeva R, Miftakhova R, Gomzikova M, Ignatyev Y, Petukhov A, Davidovich P, Rizvanov A, Barlev NA. Cell Death Discov. 2018 Nov 13;4:103. doi: 10.1038/s41420-018-0120-z. eCollection 2018. PubMed
Histone demethylase LSD1 is required for germinal center formation and BCL6-driven lymphomagenesis. Hatzi K, Geng H, Doane AS, Meydan C, LaRiviere R, Cardenas M, Duy C, Shen H, Vidal MNC, Baslan T, Mohammad HP, Kruger RG, Shaknovich R, Haberman AM, Inghirami G, Lowe SW, Melnick AM. Nat Immunol. 2019 Jan;20(1):86-96. doi: 10.1038/s41590-018-0273-1. Epub 2018 Dec 10. PubMed
The circadian E3 ligase complex SCF(FBXL3+CRY) targets TLK2. Correia SP, Chan AB, Vaughan M, Zolboot N, Perea V, Huber AL, Kriebs A, Moresco JJ, Yates JR 3rd, Lamia KA. Sci Rep. 2019 Jan 17;9(1):198. doi: 10.1038/s41598-018-36618-3. PubMed
Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays. Deshpande A, Chen BR, Zhao L, Saddoris K, Kerr M, Zhu N, Mali P, Deshpande AJ. J Vis Exp. 2019 Jan 7;(143). doi: 10.3791/58710. PubMed
Uncoupling protein 2 reprograms the tumor microenvironment to support the anti-tumor immune cycle. Cheng WC, Tsui YC, Ragusa S, Koelzer VH, Mina M, Franco F, Laubli H, Tschumi B, Speiser D, Romero P, Zippelius A, Petrova TV, Mertz K, Ciriello G, Ho PC. Nat Immunol. 2019 Feb;20(2):206-217. doi: 10.1038/s41590-018-0290-0. Epub 2019 Jan 21. PubMed
Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines. Webster DE, Roulland S, Phelan JD. Methods Mol Biol. 2019;1956:337-350. doi: 10.1007/978-1-4939-9151-8_16. PubMed
A KLF6-driven transcriptional network links lipid homeostasis and tumour growth in renal carcinoma. Syafruddin SE, Rodrigues P, Vojtasova E, Patel SA, Zaini MN, Burge J, Warren AY, Stewart GD, Eisen T, Bihary D, Samarajiwa SA, Vanharanta S. Nat Commun. 2019 Mar 11;10(1):1152. doi: 10.1038/s41467-019-09116-x. PubMed
Artemisinin compounds sensitize cancer cells to ferroptosis by regulating iron homeostasis. Chen GQ, Benthani FA, Wu J, Liang D, Bian ZX, Jiang X. Cell Death Differ. 2019 May 21. pii: 10.1038/s41418-019-0352-3. doi: 10.1038/s41418-019-0352-3. PubMed
Comprehensive substrate specificity profiling of the human Nek kinome reveals unexpected signaling outputs. van de Kooij B, Creixell P, van Vlimmeren A, Joughin BA, Miller CJ, Haider N, Simpson CD, Linding R, Stambolic V, Turk BE, Yaffe MB. Elife. 2019 May 24;8. pii: 44635. doi: 10.7554/eLife.44635. PubMed
Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. Artegiani B, van Voorthuijsen L, Lindeboom RGH, Seinstra D, Heo I, Tapia P, Lopez-Iglesias C, Postrach D, Dayton T, Oka R, Hu H, van Boxtel R, van Es JH, Offerhaus J, Peters PJ, van Rheenen J, Vermeulen M, Clevers H. Cell Stem Cell. 2019 Jun 6;24(6):927-943.e6. doi: 10.1016/j.stem.2019.04.017. Epub 2019 May 23. PubMed
FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes. Adams EJ, Karthaus WR, Hoover E, Liu D, Gruet A, Zhang Z, Cho H, DiLoreto R, Chhangawala S, Liu Y, Watson PA, Davicioni E, Sboner A, Barbieri CE, Bose R, Leslie CS, Sawyers CL. Nature. 2019 Jul;571(7765):408-412. doi: 10.1038/s41586-019-1318-9. Epub 2019 Jun 26. PubMed
Reversible Regulation of Polyubiquitin Gene UBC via Modified Inducible CRISPR/Cas9 System. Han SW, Jung BK, Park SH, Ryu KY. Int J Mol Sci. 2019 Jun 28;20(13). pii: ijms20133168. doi: 10.3390/ijms20133168. PubMed
Poly (ADP) ribose glycohydrolase can be effectively targeted in pancreatic cancer. Jain A, Agostini LC, McCarthy GA, Chand SN, Ramirez A, Nevler A, Cozzitorto J, Schultz CW, Lowder CY, Smith KM, Waddell ID, Raitses-Gurevich M, Stossel C, Gorman YG, Atias D, Yeo CJ, Winter JM, Olive KP, Golan T, Pishvaian MJ, Ogilvie D, James DI, Jordan AM, Brody JR. Cancer Res. 2019 Jul 4. pii: 0008-5472.CAN-18-3645. doi: 10.1158/0008-5472.CAN-18-3645. PubMed
Intercellular interaction dictates cancer cell ferroptosis via NF2-YAP signalling. Wu J, Minikes AM, Gao M, Bian H, Li Y, Stockwell BR, Chen ZN, Jiang X. Nature. 2019 Aug;572(7769):402-406. doi: 10.1038/s41586-019-1426-6. Epub 2019 Jul 24. PubMed
The Barrier Molecules Junction Plakoglobin, Filaggrin, and Dystonin Play Roles in Melanoma Growth and Angiogenesis. Leick KM, Rodriguez AB, Melssen MM, Benamar M, Lindsay RS, Eki R, Du KP, Parlak M, Abbas T, Engelhard VH, Slingluff CL Jr. Ann Surg. 2019 Oct;270(4):712-722. doi: 10.1097/SLA.0000000000003522. PubMed
Ex vivo screen identifies CDK12 as a metastatic vulnerability in osteosarcoma. Bayles I, Krajewska M, Pontius WD, Saiakhova A, Morrow JJ, Bartels C, Lu J, Faber ZJ, Fedorov Y, Hong ES, Karnuta JM, Rubin B, Adams DJ, George RE, Scacheri PC. J Clin Invest. 2019 Oct 1;129(10):4377-4392. doi: 10.1172/JCI127718. PubMed
IFN-gamma-induced PD-L1 expression in melanoma depends on p53 expression. Thiem A, Hesbacher S, Kneitz H, di Primio T, Heppt MV, Hermanns HM, Goebeler M, Meierjohann S, Houben R, Schrama D. J Exp Clin Cancer Res. 2019 Sep 11;38(1):397. doi: 10.1186/s13046-019-1403-9. PubMed
Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma. Meyer SN, Scuoppo C, Vlasevska S, Bal E, Holmes AB, Holloman M, Garcia-Ibanez L, Nataraj S, Duval R, Vantrimpont T, Basso K, Brooks N, Dalla-Favera R, Pasqualucci L. Immunity. 2019 Sep 17;51(3):535-547.e9. doi: 10.1016/j.immuni.2019.08.006. Epub 2019 Sep 10. PubMed
Progenitor-derived human endothelial cells evade alloimmunity by CRISPR/Cas9-mediated complete ablation of MHC expression. Merola J, Reschke M, Pierce RW, Qin L, Spindler S, Baltazar T, Manes TD, Lopez-Giraldez F, Li G, Bracaglia LG, Xie C, Kirkiles-Smith N, Saltzman WM, Tietjen GT, Tellides G, Pober JS. JCI Insight. 2019 Oct 17;4(20). pii: 129739. doi: 10.1172/jci.insight.129739. PubMed
BAP1 regulates epigenetic switch from pluripotency to differentiation in developmental lineages giving rise to BAP1-mutant cancers. Kuznetsov JN, Aguero TH, Owens DA, Kurtenbach S, Field MG, Durante MA, Rodriguez DA, King ML, Harbour JW. Sci Adv. 2019 Sep 18;5(9):eaax1738. doi: 10.1126/sciadv.aax1738. eCollection 2019 Sep. PubMed
The MTH1 inhibitor TH588 is a microtubule-modulating agent that eliminates cancer cells by activating the mitotic surveillance pathway. Gul N, Karlsson J, Tangemo C, Linsefors S, Tuyizere S, Perkins R, Ala C, Zou Z, Larsson E, Bergo MO, Lindahl P. Sci Rep. 2019 Oct 11;9(1):14667. doi: 10.1038/s41598-019-51205-w. PubMed
An improved platform for functional assessment of large protein libraries in mammalian cells. Matreyek KA, Stephany JJ, Chiasson MA, Hasle N, Fowler DM. Nucleic Acids Res. 2020 Jan 10;48(1):e1. doi: 10.1093/nar/gkz910. PubMed
Patient-Derived Head and Neck Cancer Organoids Recapitulate EGFR Expression Levels of Respective Tissues and Are Responsive to EGFR-Targeted Photodynamic Therapy. Driehuis E, Spelier S, Beltran Hernandez I, de Bree R, M Willems S, Clevers H, Oliveira S. J Clin Med. 2019 Nov 5;8(11). pii: jcm8111880. doi: 10.3390/jcm8111880. PubMed
IRG1 and Inducible Nitric Oxide Synthase Act Redundantly with Other Interferon-Gamma-Induced Factors To Restrict Intracellular Replication of Legionella pneumophila. Price JV, Russo D, Ji DX, Chavez RA, DiPeso L, Lee AY, Coers J, Vance RE. mBio. 2019 Nov 12;10(6). pii: mBio.02629-19. doi: 10.1128/mBio.02629-19. PubMed
CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci. Chylinski K, Hubmann M, Hanna RE, Yanchus C, Michlits G, Uijttewaal ECH, Doench J, Schramek D, Elling U. Nat Commun. 2019 Nov 29;10(1):5454. doi: 10.1038/s41467-019-13403-y. PubMed
Induction of an Alternative mRNA 5' Leader Enhances Translation of the Ciliopathy Gene Inpp5e and Resistance to Oncolytic Virus Infection. Hoang HD, Graber TE, Jia JJ, Vaidya N, Gilchrist VH, Xiang X, Li W, Cowan KN, Gkogkas CG, Jaramillo M, Jafarnejad SM, Alain T. Cell Rep. 2019 Dec 17;29(12):4010-4023.e5. doi: 10.1016/j.celrep.2019.11.072. PubMed
Construction of an Inducible CRISPR/Cas9 System for CXCR4 Gene and Demonstration of its Effects on MKN-45 Cells. Peng Y, Yang T, Tang X, Chen F, Wang S. Cell Biochem Biophys. 2019 Dec 24. pii: 10.1007/s12013-019-00898-x. doi: 10.1007/s12013-019-00898-x. PubMed
A solid-phase transfection platform for arrayed CRISPR screens. Sercin O, Reither S, Roidos P, Ballin N, Palikyras S, Baginska A, Rein K, Llamazares M, Halavatyi A, Winter H, Muley T, Jurkowska RZ, Abdollahi A, Zenke FT, Neumann B, Mardin BR. Mol Syst Biol. 2019 Dec;15(12):e8983. doi: 10.15252/msb.20198983. PubMed
Establishment of A Reversibly Inducible Porcine Granulosa Cell Line. Bai Y, Zhu C, Feng M, Pan B, Zhang S, Zhan X, Chen H, Wang B, Li J. Cells. 2020 Jan 8;9(1). pii: cells9010156. doi: 10.3390/cells9010156. PubMed
West Nile virus capsid protein inhibits autophagy by AMP-activated protein kinase degradation in neurological disease development. Kobayashi S, Yoshii K, Phongphaew W, Muto M, Hirano M, Orba Y, Sawa H, Kariwa H. PLoS Pathog. 2020 Jan 23;16(1):e1008238. doi: 10.1371/journal.ppat.1008238. eCollection 2020 Jan. PubMed
Cellular context- and protein level-dependent interaction of pluripotency factor OCT4A with multiple octamer motifs of the same target gene. Zhou N, Zhang XB, Chen C, Chen XY, Kang B, He JQ, Gong GZ, Wang YJ, Zhou YW. Life Sci. 2020 May 1;248:117461. doi: 10.1016/j.lfs.2020.117461. Epub 2020 Feb 22. PubMed
Electron transport chain activity is a predictor and target for venetoclax sensitivity in multiple myeloma. Bajpai R, Sharma A, Achreja A, Edgar CL, Wei C, Siddiqa AA, Gupta VA, Matulis SM, McBrayer SK, Mittal A, Rupji M, Barwick BG, Lonial S, Nooka AK, Boise LH, Nagrath D, Shanmugam M. Nat Commun. 2020 Mar 6;11(1):1228. doi: 10.1038/s41467-020-15051-z. PubMed
Live cell imaging of single RNA molecules with fluorogenic Mango II arrays. Cawte AD, Unrau PJ, Rueda DS. Nat Commun. 2020 Mar 9;11(1):1283. doi: 10.1038/s41467-020-14932-7. PubMed

Associated Plasmids

Telomere length heterogeneity in ALT cells is maintained by PML-dependent localization of the BTR complex to telomeres. Loe TK, Li JSZ, Zhang Y, Azeroglu B, Boddy MN, Denchi EL. Genes Dev. 2020 May 1;34(9-10):650-662. doi: 10.1101/gad.333963.119. Epub 2020 Mar 26. PubMed
TIE2 Induces Breast Cancer Cell Dormancy and Inhibits the Development of Osteolytic Bone Metastases. Drescher F, Juarez P, Arellano DL, Serafin-Higuera N, Olvera-Rodriguez F, Jimenez S, Licea-Navarro AF, Fournier PG. Cancers (Basel). 2020 Apr 3;12(4). pii: cancers12040868. doi: 10.3390/cancers12040868. PubMed
Phenotype instability of hepatocyte-like cells produced by direct reprogramming of mesenchymal stromal cells. Orge ID, Gadd VL, Barouh JL, Rossi EA, Carvalho RH, Smith I, Allahdadi KJ, Paredes BD, Silva DN, Damasceno PKF, Sampaio GL, Forbes SJ, Soares MBP, Souza BSF. Stem Cell Res Ther. 2020 Apr 10;11(1):154. doi: 10.1186/s13287-020-01665-z. PubMed
miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro. Przanowska RK, Sobierajska E, Su Z, Jensen K, Przanowski P, Nagdas S, Kashatus JA, Kashatus DF, Bhatnagar S, Lukens JR, Dutta A. FASEB J. 2020 Jun;34(6):7687-7702. doi: 10.1096/fj.201902855RR. Epub 2020 Apr 11. PubMed
Identification of calcium and integrin-binding protein 1 as a novel regulator of production of amyloid beta peptide using CRISPR/Cas9-based screening system. Chiu YW, Hori Y, Ebinuma I, Sato H, Hara N, Ikeuchi T, Tomita T. FASEB J. 2020 Jun;34(6):7661-7674. doi: 10.1096/fj.201902966RR. Epub 2020 Apr 19. PubMed
Distinct roles of BRCA2 in replication fork protection in response to hydroxyurea and DNA interstrand cross-links. Rickman KA, Noonan RJ, Lach FP, Sridhar S, Wang AT, Abhyankar A, Huang A, Kelly M, Auerbach AD, Smogorzewska A. Genes Dev. 2020 Jun 1;34(11-12):832-846. doi: 10.1101/gad.336446.120. Epub 2020 Apr 30. PubMed
Targeting RLIP with CRISPR/Cas9 controls tumor growth. Singhal J, Chikara S, Horne D, Awasthi S, Salgia R, Singhal SS. Carcinogenesis. 2020 May 19. pii: 5840500. doi: 10.1093/carcin/bgaa048. PubMed
Tumor Cell Associated Hyaluronan-CD44 Signaling Promotes Pro-Tumor Inflammation in Breast Cancer. Witschen PM, Chaffee TS, Brady NJ, Huggins DN, Knutson TP, LaRue RS, Munro SA, Tiegs L, McCarthy JB, Nelson AC, Schwertfeger KL. Cancers (Basel). 2020 May 22;12(5). pii: cancers12051325. doi: 10.3390/cancers12051325. PubMed
Filoviruses Use the HOPS Complex and UVRAG To Traffic to Niemann-Pick C1 Compartments during Viral Entry. Bo Y, Qiu S, Mulloy RP, Cote M. J Virol. 2020 Jul 30;94(16). pii: JVI.01002-20. doi: 10.1128/JVI.01002-20. Print 2020 Jul 30. PubMed
Dysfunctional telomeres trigger cellular senescence mediated by cyclic GMP-AMP synthase. Abdisalaam S, Bhattacharya S, Mukherjee S, Sinha D, Srinivasan K, Zhu M, Akbay EA, Sadek HA, Shay JW, Asaithamby A. J Biol Chem. 2020 Aug 7;295(32):11144-11160. doi: 10.1074/jbc.RA120.012962. Epub 2020 Jun 15. PubMed
Dual Role of WISP1 in maintaining glioma stem cells and tumor-supportive macrophages in glioblastoma. Tao W, Chu C, Zhou W, Huang Z, Zhai K, Fang X, Huang Q, Zhang A, Wang X, Yu X, Huang H, Wu Q, Sloan AE, Yu JS, Li X, Stark GR, Rich JN, Bao S. Nat Commun. 2020 Jun 15;11(1):3015. doi: 10.1038/s41467-020-16827-z. PubMed
The c-Rel-c-Myc axis controls metabolism and proliferation of human T leukemia cells. Li X, Luo G, Li T, Sun H, Wang W, Eiler E, Goldsmith JR, Chen YH. Mol Immunol. 2020 Sep;125:115-122. doi: 10.1016/j.molimm.2020.06.029. Epub 2020 Jul 10. PubMed

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